FastQCFastQC Report
Thu 2 Feb 2017
SRR4064169_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064169_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268516
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA16700.6219368678216568No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9550.3556585082453187No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA9130.3400169822282471No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG8120.3024028363300511No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC7510.2796853818766852No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7500.2793129645905644No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA7070.2632990212873721No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT6750.251381668131508No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA6710.24989199898702497No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA6400.23834706311728165No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5500.20482950736641392No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA5070.18881556406322156No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4800.17876029733796125No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA4730.17615337633511596No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT4690.17466370719063296No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC4420.16460844046537265No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA4300.1601394330319236No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC4090.1523186700233878No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA4080.15194625273726706No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC3950.14710482801769728No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGG3820.1422634032981275No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC3550.13220813657286717No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTC3540.13183571928674642No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT3530.13146330200062567No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGAC3520.13109088471450492No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGAT3510.13071846742838414No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA3510.13071846742838414No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTT3340.12438737356433135No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC3290.1225252871337276No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAA3280.12215286984760684No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA3280.12215286984760684No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGT3270.12178045256148609No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC3240.12066320070312385No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC3210.11954594884476158No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA3200.11917353155864083No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG3150.11731144512803705No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA3100.1154493586974333No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3090.11507694141131256No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGT3080.11470452412519179No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGATAGAAACCGACC3050.11358727226682955No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA3030.11284243769458804No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG3010.11209760312234653No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC2930.10911826483338051No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA2820.10502167468605222No Hit
ATTTAAGAGTTCATATCGACAATTAGGGTTTACGACCTCGATGTTGGATC2800.10427684011381072No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA2790.10390442282768998No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCTTGTTTGCCGAGT2780.10353200554156922No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2770.10315958825544848No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA2730.10166991911096546No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC2710.10092508453872395No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAGG207.8489643E-443.9962731
TCCCCGG250.002348373635.20357541
TATGTCG401.8158818E-532.99720416
GCCCCCG300.005734309629.33631343
GTCCTAT602.9331386E-729.3308531
GCGAGCA300.005739532429.3308539
TGTCGAG554.945463E-627.99763118
CGAATTT407.0136285E-427.50279422
ACGAATT407.0136285E-427.50279421
TGTACGA407.0212706E-427.49767118
GTACGAA407.0212706E-427.49767119
ATGTCGA508.3225175E-526.39776617
TACGAAT450.0013945624.44692820
GATGTAC450.001396074424.44237516
AGTGGTC450.001396074424.4423758
GTCCTAA2500.023.757991
CCTACGT651.8051647E-523.69471443
ATGGGTA651.8077699E-523.6903025
AACTTGC500.002576527421.9981387
TAGAAGT602.866366E-421.9981384
Produced by FastQC (version 0.11.5)