##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064169_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	268516
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.582337737788436	32.0	32.0	32.0	32.0	32.0
2	31.164898925948545	32.0	32.0	32.0	32.0	32.0
3	31.335145764125787	32.0	32.0	32.0	32.0	32.0
4	31.43101342191899	32.0	32.0	32.0	32.0	32.0
5	31.31134457536981	32.0	32.0	32.0	32.0	32.0
6	34.823261928525675	36.0	36.0	36.0	32.0	36.0
7	34.88103874629445	36.0	36.0	36.0	36.0	36.0
8	34.864257623381846	36.0	36.0	36.0	32.0	36.0
9	34.97730116641094	36.0	36.0	36.0	36.0	36.0
10	34.83757764900415	36.0	36.0	36.0	32.0	36.0
11	34.988376856500174	36.0	36.0	36.0	36.0	36.0
12	34.90595346273593	36.0	36.0	36.0	32.0	36.0
13	34.945239762248804	36.0	36.0	36.0	36.0	36.0
14	34.88423408660936	36.0	36.0	36.0	32.0	36.0
15	34.85987054775134	36.0	36.0	36.0	32.0	36.0
16	34.84765898493944	36.0	36.0	36.0	32.0	36.0
17	34.81509481744104	36.0	36.0	36.0	32.0	36.0
18	34.8253623620194	36.0	36.0	36.0	32.0	36.0
19	34.75935139805449	36.0	36.0	36.0	32.0	36.0
20	34.70825947057158	36.0	36.0	36.0	32.0	36.0
21	34.68433165993833	36.0	36.0	36.0	32.0	36.0
22	34.67432853163312	36.0	36.0	36.0	32.0	36.0
23	34.67455943035052	36.0	36.0	36.0	32.0	36.0
24	34.66712970549241	36.0	36.0	36.0	32.0	36.0
25	34.67103636282382	36.0	36.0	36.0	32.0	36.0
26	34.5728373728195	36.0	36.0	36.0	32.0	36.0
27	34.57189888125847	36.0	36.0	36.0	32.0	36.0
28	34.55885310372566	36.0	36.0	36.0	32.0	36.0
29	34.50207064011083	36.0	36.0	36.0	32.0	36.0
30	34.45189858332464	36.0	36.0	36.0	32.0	36.0
31	34.41077626659119	36.0	36.0	36.0	32.0	36.0
32	34.31475591771068	36.0	36.0	36.0	32.0	36.0
33	34.234637786947516	36.0	36.0	36.0	32.0	36.0
34	34.110682417435086	36.0	36.0	36.0	32.0	36.0
35	34.00064428190499	36.0	36.0	36.0	32.0	36.0
36	33.924812674105084	36.0	36.0	36.0	32.0	36.0
37	33.87110265310075	36.0	36.0	36.0	32.0	36.0
38	33.78869043185509	36.0	36.0	36.0	32.0	36.0
39	33.653275782448716	36.0	36.0	36.0	27.0	36.0
40	33.61878994175394	36.0	36.0	36.0	27.0	36.0
41	33.54732678872023	36.0	36.0	36.0	21.0	36.0
42	33.444476306812255	36.0	36.0	36.0	21.0	36.0
43	33.345014822207986	36.0	36.0	36.0	21.0	36.0
44	33.223707339599876	36.0	36.0	36.0	14.0	36.0
45	33.02340270225983	36.0	36.0	36.0	14.0	36.0
46	32.898110354690225	36.0	36.0	36.0	14.0	36.0
47	32.756930685694705	36.0	36.0	36.0	14.0	36.0
48	32.62437620104575	36.0	36.0	36.0	14.0	36.0
49	32.50816338691177	36.0	36.0	36.0	14.0	36.0
50	31.825857677009935	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	7.0
22	43.0
23	128.0
24	343.0
25	776.0
26	1591.0
27	3057.0
28	5357.0
29	7980.0
30	10959.0
31	14987.0
32	20468.0
33	30689.0
34	53492.0
35	118639.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.331548013676695	21.42048374961823	13.265868611398732	20.982099625306347
2	12.211235871366592	23.559205258551664	43.6500754147813	20.579483455300448
3	16.923449341749997	32.0324749082919	31.195277731225445	19.84879801873266
4	9.19461037703526	18.620864306037628	40.54246301896349	31.642062297963626
5	9.365549911364687	43.38251724292035	34.428860850005215	12.823071995709753
6	25.343368737803335	41.10146136543074	21.10190826617408	12.453261630591845
7	24.927006211920332	35.666031074498356	22.992298410523023	16.41466430305829
8	26.591711480880097	34.60650389548481	21.515291453768118	17.286493169866972
9	28.332811941365748	14.371052317959718	22.70103384578715	34.59510189488738
10	15.427758494838296	28.497370733959986	34.765526076658375	21.30934469454334
11	31.248789643820107	24.11401927631873	27.149220158202862	17.4879709216583
12	23.640392529281417	28.358192279760907	32.474908291901755	15.526506899055919
13	27.78876412863341	23.75249054987617	29.393516190901813	19.065229130588605
14	21.167160121408486	24.04260469619947	31.29136174887809	23.498873433513957
15	21.633780995582967	31.43877604224859	29.36954853895257	17.55789442321587
16	19.33872847869563	29.388148804713367	30.871131006692416	20.40199170989859
17	17.195995769339632	29.242950140773733	31.889347375947803	21.671706713938836
18	17.584799416049695	27.263552265041934	36.66597148773258	18.485676831175795
19	20.96150694930656	27.803557329917027	33.19913897123449	18.035796749541927
20	22.177531981453548	26.71508109416606	33.57577788950338	17.531609034877008
21	23.15392751269943	25.513191020274395	30.956442074215317	20.37643939281086
22	21.147128268917076	28.861437392463706	31.028922141862246	18.96251219675697
23	19.49693498290592	29.094199929985027	33.03850078580632	18.370364301302725
24	20.23954058261394	29.613353493672584	32.50482283977744	17.642283083936032
25	20.19827717838615	28.678685948166383	33.127260132656524	17.995776740790948
26	17.618994413407822	29.288640595903164	34.69459962756052	18.39776536312849
27	18.546714091510083	30.69479878582469	33.21812324251848	17.540363880146746
28	17.935167184345975	29.658927547242893	33.37467319166052	19.031232076750612
29	18.358788718441467	29.012062910367998	33.30465645471846	19.324491916472073
30	19.176107796473822	28.890974503363054	33.19739596135654	18.735521738806582
31	18.954382253474964	28.884287055107787	32.59463969131296	19.566691000104285
32	16.846550915063354	31.207496294309994	32.955688141047126	18.990264649579526
33	17.950837988826816	30.607448789571695	32.890875232774675	18.550837988826814
34	18.034874972998338	30.48729618401353	33.9044029467192	17.57342589626893
35	19.34861548202082	31.167389806521296	31.132381147464667	18.351613563993222
36	18.261556167199892	30.82352240095645	31.982584275940155	18.932337155903507
37	18.941617257098592	31.39729761940232	29.818177755266216	19.842907368232876
38	18.47143958495499	33.24270673107363	30.301563122669933	17.984290561301446
39	18.486806577158713	31.97147166719428	30.01471108545465	19.52701067019236
40	19.799336335094914	32.200782841543486	31.0108785925239	16.9890022308377
41	19.20150462747435	31.195322247257966	31.2470903707566	18.35608275451109
42	18.42172888059618	33.98098387763538	30.25485179267734	17.34243544909109
43	17.33040353066051	33.692110016573245	29.94134187445299	19.03614457831325
44	17.753768919079967	32.46819508997736	30.644812894768204	19.133223096174472
45	18.525939443596144	32.561915757327476	29.89981751145209	19.0123272876243
46	19.693200354543897	31.688105648122633	28.440230304565123	20.178463692768347
47	20.90932452412786	31.858387304614034	27.99927003765265	19.233018133605455
48	18.613762564662157	33.83958199105437	27.656967498538236	19.88968794574524
49	19.086900496808358	32.01918708149296	29.151180199170245	19.742732222528435
50	17.636324362975486	33.907729205925946	27.73970817164096	20.716238259457604
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	73.0
1	50.0
2	27.0
3	126.0
4	225.0
5	259.5
6	294.0
7	298.0
8	302.0
9	408.5
10	515.0
11	748.5
12	982.0
13	1665.0
14	2348.0
15	3303.5
16	4259.0
17	4816.0
18	5373.0
19	5523.0
20	5673.0
21	6158.5
22	6644.0
23	6819.0
24	6994.0
25	6716.0
26	6438.0
27	7728.0
28	9018.0
29	10067.0
30	11116.0
31	11413.0
32	11710.0
33	13805.5
34	15901.0
35	18480.5
36	21060.0
37	22274.5
38	23489.0
39	21631.5
40	19774.0
41	19449.0
42	19124.0
43	17158.5
44	15193.0
45	15480.5
46	15768.0
47	15664.0
48	15560.0
49	13486.5
50	11413.0
51	10181.0
52	8949.0
53	7695.5
54	6442.0
55	5893.5
56	5345.0
57	5021.0
58	4697.0
59	4075.0
60	3453.0
61	2984.5
62	2516.0
63	2225.5
64	1935.0
65	1813.5
66	1692.0
67	1468.5
68	1245.0
69	1144.0
70	1043.0
71	872.5
72	702.0
73	583.0
74	464.0
75	396.5
76	329.0
77	266.0
78	203.0
79	159.5
80	116.0
81	85.5
82	55.0
83	41.0
84	27.0
85	19.5
86	12.0
87	10.0
88	8.0
89	4.0
90	0.0
91	1.5
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	2.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.011172518583622578
2	3.7241728612075263E-4
3	3.7241728612075263E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0014896691444830105
10	0.0
11	0.0
12	3.7241728612075263E-4
13	3.7241728612075263E-4
14	3.7241728612075263E-4
15	0.003724172861207526
16	0.0011172518583622579
17	0.0
18	0.0
19	0.0
20	3.7241728612075263E-4
21	0.0
22	7.448345722415053E-4
23	7.448345722415053E-4
24	7.448345722415053E-4
25	0.0011172518583622579
26	0.005958676577932042
27	0.007820763008535804
28	0.003724172861207526
29	0.002606921002845268
30	0.003724172861207526
31	0.008938014866898063
32	0.003724172861207526
33	0.005958676577932042
34	0.005213842005690536
35	0.004096590147328279
36	0.00856559758077731
37	0.0044690074334490315
38	0.005586259291811289
39	0.004096590147328279
40	0.002606921002845268
41	0.004096590147328279
42	0.002606921002845268
43	0.004096590147328279
44	0.0014896691444830105
45	0.0022345037167245158
46	7.448345722415053E-4
47	0.0033517555750867734
48	0.001862086430603763
49	7.448345722415053E-4
50	7.448345722415053E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	268516.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.19957525360496
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.65475378594724	29.521454208826682
2	15.41363306350893	12.700702706629796
3	5.304770445325159	6.556628674958202
4	2.414324194634298	3.9787652537373983
5	1.2952173415332369	2.6681202166136377
6	0.7724869012371126	1.9095679331965518
7	0.511943185083292	1.476428924558651
8	0.3485444097653535	1.1487905311480804
9	0.27229793358479576	1.0096703285515127
>10	1.5525891440363606	12.583517499730256
>50	0.25051340004760037	7.128048660846649
>100	0.1970441367963339	15.40161100793249
>500	0.010891886958591323	3.235423449274583
>1k	9.901715416901203E-4	0.6812706039955305
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1670	0.6219368678216568	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	955	0.3556585082453187	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	913	0.3400169822282471	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	812	0.3024028363300511	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	751	0.2796853818766852	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	750	0.2793129645905644	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	707	0.2632990212873721	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	675	0.251381668131508	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	671	0.24989199898702497	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	640	0.23834706311728165	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	550	0.20482950736641392	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA	507	0.18881556406322156	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	480	0.17876029733796125	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	473	0.17615337633511596	No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT	469	0.17466370719063296	No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC	442	0.16460844046537265	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA	430	0.1601394330319236	No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC	409	0.1523186700233878	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	408	0.15194625273726706	No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC	395	0.14710482801769728	No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGG	382	0.1422634032981275	No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC	355	0.13220813657286717	No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTC	354	0.13183571928674642	No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT	353	0.13146330200062567	No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGAC	352	0.13109088471450492	No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGAT	351	0.13071846742838414	No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA	351	0.13071846742838414	No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTT	334	0.12438737356433135	No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC	329	0.1225252871337276	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAA	328	0.12215286984760684	No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA	328	0.12215286984760684	No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGT	327	0.12178045256148609	No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC	324	0.12066320070312385	No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC	321	0.11954594884476158	No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA	320	0.11917353155864083	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	315	0.11731144512803705	No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA	310	0.1154493586974333	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	309	0.11507694141131256	No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGT	308	0.11470452412519179	No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGATAGAAACCGACC	305	0.11358727226682955	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	303	0.11284243769458804	No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG	301	0.11209760312234653	No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC	293	0.10911826483338051	No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	282	0.10502167468605222	No Hit
ATTTAAGAGTTCATATCGACAATTAGGGTTTACGACCTCGATGTTGGATC	280	0.10427684011381072	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	279	0.10390442282768998	No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCTTGTTTGCCGAGT	278	0.10353200554156922	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	277	0.10315958825544848	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	273	0.10166991911096546	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	271	0.10092508453872395	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	3.724172861207526E-4	0.0
19	0.0	0.0	0.0	3.724172861207526E-4	0.0
20	0.0	0.0	0.0	3.724172861207526E-4	0.0
21	0.0	0.0	0.0	3.724172861207526E-4	0.0
22	0.0	0.0	0.0	0.001862086430603763	0.0
23	0.0	0.0	0.0	0.007448345722415052	0.0
24	0.0	0.0	0.0	0.011172518583622577	0.0
25	0.0	0.0	0.0	0.01601394330319236	0.0
26	0.0	0.0	0.0	0.018248447019916878	0.0
27	0.0	0.0	0.0	0.023834706311728165	0.0
28	0.0	0.0	0.0	0.033517555750867734	0.0
29	0.0	0.0	0.0	0.0432004051900073	0.0
30	0.0	0.0	0.0	0.07038686707682223	0.0
31	0.0	0.0	0.0	0.11693902784191632	0.0
32	0.0	0.0	0.0	0.16944986518494243	0.0
33	0.0	0.0	0.0	0.21562960866391576	0.0
34	0.0	0.0	0.0	0.26292660400125134	0.0
35	0.0	0.0	0.0	0.3210237006360887	0.0
36	0.0	0.0	0.0	0.3999761652936883	0.0
37	0.0	0.0	0.0	0.5239911215718989	0.0
38	0.0	0.0	0.0	0.6550820062864038	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCTAGG	20	7.8489643E-4	43.996273	1
TCCCCGG	25	0.0023483736	35.203575	41
TATGTCG	40	1.8158818E-5	32.997204	16
GCCCCCG	30	0.0057343096	29.336313	43
GTCCTAT	60	2.9331386E-7	29.330853	1
GCGAGCA	30	0.0057395324	29.330853	9
TGTCGAG	55	4.945463E-6	27.997631	18
CGAATTT	40	7.0136285E-4	27.502794	22
ACGAATT	40	7.0136285E-4	27.502794	21
TGTACGA	40	7.0212706E-4	27.497671	18
GTACGAA	40	7.0212706E-4	27.497671	19
ATGTCGA	50	8.3225175E-5	26.397766	17
TACGAAT	45	0.00139456	24.446928	20
GATGTAC	45	0.0013960744	24.442375	16
AGTGGTC	45	0.0013960744	24.442375	8
GTCCTAA	250	0.0	23.75799	1
CCTACGT	65	1.8051647E-5	23.694714	43
ATGGGTA	65	1.8077699E-5	23.690302	5
AACTTGC	50	0.0025765274	21.998138	7
TAGAAGT	60	2.866366E-4	21.998138	4
>>END_MODULE