FastQCFastQC Report
Thu 2 Feb 2017
SRR4064168_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064168_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2543136
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48120.189215205164018No Hit
TATCAACGCAGAGTACTTTTTTTTT28130.11061146552917343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23850.013.9396681
GGTATCA7050.013.7430731
GGACCGA1155.336915E-912.3903436
CGGTCCA2650.011.47193610
CGATAAC1604.5474735E-1111.28148310
CGTCGTA2450.011.245131510
CCGATAA1701.0913936E-1111.1769229
ACGGTAT2250.010.9782219
ATATACG2250.010.9769256
GACGGAC3300.010.9394057
GTATAAT1408.374627E-910.8558721
AAGACGG3500.010.85565955
TAACGAA1502.1336746E-910.76731313
GTCTTAC1158.836305E-710.7378741
CGAACGA1605.4387783E-1010.68898316
CGGACCA3200.010.6879319
TACCGTC2850.010.6666777
TCATATA1252.2515087E-710.63666252
CGTTATT1701.4006218E-1010.6143172
TACGCTA2250.010.5559829
Produced by FastQC (version 0.11.5)