##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064168_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2543136
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31751585444113	32.0	32.0	32.0	32.0	32.0
2	30.966507493110868	32.0	32.0	32.0	32.0	32.0
3	30.967308865904144	32.0	32.0	32.0	32.0	32.0
4	30.99524327444541	32.0	32.0	32.0	32.0	32.0
5	30.92167662287821	32.0	32.0	32.0	32.0	32.0
6	34.58601348885785	36.0	36.0	36.0	32.0	36.0
7	34.54441642130032	36.0	36.0	36.0	32.0	36.0
8	34.51482932882866	36.0	36.0	36.0	32.0	36.0
9	34.63004023379009	36.0	36.0	36.0	32.0	36.0
10	34.363812631333914	36.0	36.0	36.0	32.0	36.0
11	34.589729766713226	36.0	36.0	36.0	32.0	36.0
12	34.43968155851673	36.0	36.0	36.0	32.0	36.0
13	34.49944910535653	36.0	36.0	36.0	32.0	36.0
14	34.4097795005851	36.0	36.0	36.0	32.0	36.0
15	34.35793602858833	36.0	36.0	36.0	32.0	36.0
16	34.35368773042417	36.0	36.0	36.0	32.0	36.0
17	34.293832103355854	36.0	36.0	36.0	32.0	36.0
18	34.28065545845759	36.0	36.0	36.0	32.0	36.0
19	34.2980190599323	36.0	36.0	36.0	32.0	36.0
20	34.27941329130648	36.0	36.0	36.0	32.0	36.0
21	34.28168135719049	36.0	36.0	36.0	32.0	36.0
22	34.255969794773065	36.0	36.0	36.0	32.0	36.0
23	34.19134918462874	36.0	36.0	36.0	32.0	36.0
24	34.18758257521422	36.0	36.0	36.0	32.0	36.0
25	33.793534832584655	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	10.0
4	18.0
5	24.0
6	111.0
7	45.0
8	155.0
9	144.0
10	229.0
11	71.0
12	165.0
13	129.0
14	201.0
15	369.0
16	687.0
17	835.0
18	1057.0
19	1410.0
20	2033.0
21	3108.0
22	4762.0
23	7603.0
24	11563.0
25	17242.0
26	25041.0
27	33737.0
28	46049.0
29	62755.0
30	83143.0
31	113282.0
32	161531.0
33	233138.0
34	518283.0
35	1214206.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.73104338111498	16.37656758925621	11.262646924614101	26.629742105014714
2	17.32222094638276	18.952114599046478	36.18731193383532	27.538352520735447
3	19.59244277561703	21.99600252132926	27.8057947318689	30.60575997118481
4	13.646519682710956	14.742411063276922	34.00267553373044	37.60839372028168
5	15.623844826474256	35.22388540993225	33.14567416516838	16.00659559842511
6	35.92731502838003	33.597096754022814	16.163541972676672	14.312046244920479
7	31.50575345094965	29.466510341344808	19.840507268065544	19.187228939639994
8	28.823674489536227	32.21646047767979	18.774021564387724	20.185843468396264
9	27.30468287436184	14.412645133777021	17.985671229826437	40.2970007620347
10	16.51361311687479	26.29339462557191	30.865565331590673	26.327426925962634
11	37.59096116682089	20.791590341120113	21.766236096160746	19.851212395898248
12	25.1556932500661	22.695212658801605	28.146207647658738	24.002886443473553
13	29.83960480575715	19.17935420916887	24.41717308634349	26.563867898730493
14	24.10887378392469	18.607501549297158	24.45371290294022	32.82991176383793
15	25.66877174041769	26.19961072332516	21.559018852810215	26.57259868344693
16	26.52719708076299	25.088471862524525	22.72650724812177	25.657823808590724
17	25.014518619243898	25.15797359421207	24.34005203095417	25.487455755589867
18	25.398643072930994	24.67236716127882	24.896514733492236	25.032475032297953
19	26.077572213619703	24.484967014199732	24.458208476553814	24.97925229562675
20	26.383739151445702	23.971182808224086	24.150434335698957	25.494643704631258
21	26.779298059355384	23.700836078352978	24.12208231399352	25.397783548298115
22	26.658132546714665	23.66191662744592	24.27222557150795	25.407725254331464
23	25.5416461698904	23.663761043663055	24.6370988370949	26.157493949351647
24	25.41415701172602	24.182566722782198	24.504581718929668	25.89869454656212
25	25.57418852154203	23.87431897197972	24.4614049689221	26.09008753755615
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	82.0
1	82.0
2	252.0
3	422.0
4	422.0
5	422.0
6	1130.5
7	1839.0
8	1839.0
9	1839.0
10	2473.5
11	3108.0
12	3108.0
13	3108.0
14	3965.5
15	4823.0
16	4823.0
17	4823.0
18	8294.5
19	11766.0
20	11766.0
21	11766.0
22	18597.0
23	25428.0
24	25428.0
25	25428.0
26	37673.5
27	49919.0
28	49919.0
29	49919.0
30	65737.5
31	81556.0
32	81556.0
33	81556.0
34	104301.5
35	127047.0
36	127047.0
37	127047.0
38	155950.5
39	184854.0
40	184854.0
41	184854.0
42	220943.5
43	257033.0
44	257033.0
45	257033.0
46	289561.0
47	322089.0
48	322089.0
49	322089.0
50	339716.5
51	357344.0
52	357344.0
53	357344.0
54	347645.0
55	337946.0
56	337946.0
57	337946.0
58	314305.0
59	290664.0
60	290664.0
61	290664.0
62	252999.5
63	215335.0
64	215335.0
65	215335.0
66	175575.0
67	135815.0
68	135815.0
69	135815.0
70	103435.5
71	71056.0
72	71056.0
73	71056.0
74	53273.5
75	35491.0
76	35491.0
77	35491.0
78	26402.5
79	17314.0
80	17314.0
81	17314.0
82	11937.0
83	6560.0
84	6560.0
85	6560.0
86	4677.5
87	2795.0
88	2795.0
89	2795.0
90	1946.0
91	1097.0
92	1097.0
93	1097.0
94	770.0
95	443.0
96	443.0
97	443.0
98	876.5
99	1310.0
100	1310.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03995067507203705
2	0.005819586526241617
3	0.0012976105092295496
4	0.003617580813609653
5	0.008965308972858707
6	0.012858140500547356
7	0.022609880085060336
8	0.03495684068803241
9	0.04942716394247102
10	0.05634775332502862
11	0.05473557057113737
12	0.0629144489323418
13	0.06295377046292451
14	0.06531306229788733
15	0.058942974343487727
16	0.06488052546147748
17	0.06181344607602582
18	0.07679494922803971
19	0.07435701433191147
20	0.07919356259358525
21	0.07341329759792634
22	0.07722748606444957
23	0.08202471279554062
24	0.07667698463629156
25	0.07632309086104715
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2543136.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.823670008051366
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.98012349286237	23.44315738052298
2	16.115205432005183	12.190724247442418
3	7.678228248799914	8.712563145873181
4	4.1973325470853435	6.350340847000391
5	2.60833890293082	4.932847496680903
6	1.6675488257153637	3.784368990370289
7	1.1055156973215057	2.92702626469466
8	0.8006106504198073	2.422562643712813
9	0.5999378910079495	2.0422667533329872
>10	2.988171042035215	19.700106353223347
>50	0.15931329276394912	4.155705111224695
>100	0.09099377137166881	6.4170193852266095
>500	0.006484009062878863	1.6836345162828927
>1k	0.0021961966180706195	1.2376768644116456
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4812	0.189215205164018	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2813	0.11061146552917343	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	3.932153058271363E-5	3.932153058271363E-5
6	0.0	0.0	0.0	3.932153058271363E-5	3.932153058271363E-5
7	0.0	0.0	0.0	3.932153058271363E-5	3.932153058271363E-5
8	0.0	0.0	0.0	7.864306116542726E-5	3.932153058271363E-5
9	0.0	0.0	0.0	7.864306116542726E-5	3.932153058271363E-5
10	0.0	0.0	0.0	7.864306116542726E-5	7.864306116542726E-5
11	0.0	0.0	0.0	7.864306116542726E-5	7.864306116542726E-5
12	0.0	0.0	0.0	7.864306116542726E-5	1.9660765291356814E-4
13	0.0	0.0	0.0	7.864306116542726E-5	1.9660765291356814E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2385	0.0	13.939668	1
GGTATCA	705	0.0	13.743073	1
GGACCGA	115	5.336915E-9	12.390343	6
CGGTCCA	265	0.0	11.471936	10
CGATAAC	160	4.5474735E-11	11.281483	10
CGTCGTA	245	0.0	11.2451315	10
CCGATAA	170	1.0913936E-11	11.176922	9
ACGGTAT	225	0.0	10.978221	9
ATATACG	225	0.0	10.976925	6
GACGGAC	330	0.0	10.939405	7
GTATAAT	140	8.374627E-9	10.855872	1
AAGACGG	350	0.0	10.8556595	5
TAACGAA	150	2.1336746E-9	10.767313	13
GTCTTAC	115	8.836305E-7	10.737874	1
CGAACGA	160	5.4387783E-10	10.688983	16
CGGACCA	320	0.0	10.687931	9
TACCGTC	285	0.0	10.666677	7
TCATATA	125	2.2515087E-7	10.6366625	2
CGTTATT	170	1.4006218E-10	10.614317	2
TACGCTA	225	0.0	10.555982	9
>>END_MODULE