FastQCFastQC Report
Thu 2 Feb 2017
SRR4064168_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064168_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2543136
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9700.028.1266651
GTATCAA25400.027.6328241
TATAGCG500.002582291621.9984195
ATACCGT4850.018.1430246
ACCGTCG5100.018.1163438
CGTCGTA4650.017.97826210
TCAACGC40250.017.5440794
TATCAAC41150.017.2669492
CAACGCA41700.017.0395415
TAGCGTA1954.9112714E-1116.921867
ATAGCGT2251.8189894E-1216.6210256
ATCAACG42650.016.5049363
AACGCAG43650.016.379126
CGTATAT2051.05501385E-1016.09735110
TACCGTC5200.016.0757667
CGCAATA3850.016.00136636
GTCGTAG5500.015.59949111
TCGCTAG850.00298179915.52890443
CGCGGTC4150.015.3726944
CTCTTAA7550.015.1539091
Produced by FastQC (version 0.11.5)