##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064167_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2932
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.308663028649384	32.0	32.0	32.0	14.0	32.0
2	27.624488403819917	32.0	21.0	32.0	14.0	32.0
3	28.156548431105048	32.0	27.0	32.0	14.0	32.0
4	27.623465211459756	32.0	21.0	32.0	14.0	32.0
5	27.77762619372442	32.0	21.0	32.0	14.0	32.0
6	31.44031377899045	36.0	32.0	36.0	21.0	36.0
7	31.328103683492497	36.0	32.0	36.0	14.0	36.0
8	31.067530695770806	36.0	32.0	36.0	14.0	36.0
9	31.377899045020463	36.0	32.0	36.0	14.0	36.0
10	30.205320600272852	36.0	21.0	36.0	14.0	36.0
11	31.567189631650752	36.0	32.0	36.0	14.0	36.0
12	30.7950204638472	36.0	32.0	36.0	14.0	36.0
13	31.36800818553888	36.0	32.0	36.0	14.0	36.0
14	30.751023192360165	36.0	32.0	36.0	14.0	36.0
15	30.553206002728512	36.0	27.0	36.0	14.0	36.0
16	30.79638472032742	36.0	32.0	36.0	14.0	36.0
17	30.447135061391542	36.0	21.0	36.0	14.0	36.0
18	30.781036834924965	36.0	32.0	36.0	14.0	36.0
19	30.63506139154161	36.0	27.0	36.0	14.0	36.0
20	30.483969986357437	36.0	27.0	36.0	14.0	36.0
21	30.832537517053208	36.0	32.0	36.0	14.0	36.0
22	30.49045020463847	36.0	27.0	36.0	14.0	36.0
23	30.420532060027284	36.0	27.0	36.0	14.0	36.0
24	30.18656207366985	36.0	21.0	36.0	14.0	36.0
25	29.306616643929058	36.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	5.0
16	7.0
17	23.0
18	32.0
19	49.0
20	67.0
21	67.0
22	83.0
23	91.0
24	103.0
25	94.0
26	109.0
27	114.0
28	137.0
29	131.0
30	164.0
31	194.0
32	269.0
33	266.0
34	415.0
35	506.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.911262798634816	20.273037542662117	12.969283276450511	24.84641638225256
2	17.32605729877217	20.259208731241472	38.710777626193725	23.703956343792633
3	19.508867667121418	24.556616643929058	28.206002728512956	27.728512960436564
4	11.869031377899045	14.768076398362892	36.56207366984993	36.800818553888135
5	14.631650750341063	35.982264665757164	34.106412005457024	15.279672578444748
6	35.982264665757164	33.90177353342428	16.371077762619375	13.744884038199181
7	30.62755798090041	29.570259208731244	21.79399727148704	18.00818553888131
8	27.660300136425647	31.78717598908595	19.781718963165076	20.770804911323328
9	26.773533424283762	13.676671214188268	19.167803547066846	40.381991814461124
10	15.177353342428376	26.193724420190996	31.75306957708049	26.875852660300136
11	37.48294679399727	21.828103683492497	21.862210095497954	18.826739427012278
12	25.20463847203274	22.57844474761255	30.252387448840384	21.964529331514324
13	29.331514324693043	19.065484311050476	25.750341064120054	25.852660300136428
14	23.124147339699864	19.57708049113233	24.863574351978173	32.43519781718963
15	24.7612551159618	27.182810368349248	22.510231923601637	25.54570259208731
16	24.01091405184175	25.886766712141885	25.272851296043658	24.829467939972716
17	24.31787175989086	25.136425648021827	26.05729877216917	24.488403819918144
18	24.01091405184175	23.29467939972715	28.6493860845839	24.0450204638472
19	25.34106412005457	25.068212824010917	26.091405184174626	23.499317871759892
20	24.735585124530875	23.8143978164449	26.850904128283865	24.59911293074036
21	26.46657571623465	22.203274215552522	26.26193724420191	25.068212824010917
22	25.034106412005457	23.328785811732605	27.55798090040928	24.079126875852662
23	23.976807639836288	23.53342428376535	28.4106412005457	24.079126875852662
24	23.090040927694407	25.272851296043658	27.114597544338338	24.522510231923604
25	23.94270122783083	24.147339699863572	26.330150068212827	25.57980900409277
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	1.5
3	3.0
4	3.0
5	3.0
6	5.0
7	7.0
8	7.0
9	7.0
10	11.0
11	15.0
12	15.0
13	15.0
14	12.0
15	9.0
16	9.0
17	9.0
18	16.5
19	24.0
20	24.0
21	24.0
22	42.5
23	61.0
24	61.0
25	61.0
26	88.0
27	115.0
28	115.0
29	115.0
30	130.5
31	146.0
32	146.0
33	146.0
34	165.5
35	185.0
36	185.0
37	185.0
38	209.5
39	234.0
40	234.0
41	234.0
42	263.0
43	292.0
44	292.0
45	292.0
46	328.0
47	364.0
48	364.0
49	364.0
50	358.0
51	352.0
52	352.0
53	352.0
54	340.5
55	329.0
56	329.0
57	329.0
58	298.0
59	267.0
60	267.0
61	267.0
62	244.0
63	221.0
64	221.0
65	221.0
66	181.5
67	142.0
68	142.0
69	142.0
70	109.5
71	77.0
72	77.0
73	77.0
74	55.0
75	33.0
76	33.0
77	33.0
78	34.5
79	36.0
80	36.0
81	36.0
82	22.5
83	9.0
84	9.0
85	9.0
86	5.5
87	2.0
88	2.0
89	2.0
90	1.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	4.0
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06821282401091405
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.034106412005457026
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2932.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.462482946794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.94245665805975	88.71077762619373
2	2.729620066396164	5.0477489768076405
3	0.700848395426042	1.9440654843110503
4	0.2950940612320177	1.0914051841746248
5	0.07377351530800443	0.34106412005457026
6	0.11066027296200663	0.6139154160982265
7	0.0	0.0
8	0.03688675765400221	0.2728512960436562
9	0.0	0.0
>10	0.11066027296200663	1.9781718963165076
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	29	0.9890859481582538	No Hit
TATCAACGCAGAGTACTTTTTTTTT	19	0.6480218281036835	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	10	0.34106412005457026	No Hit
GGTATCAACGCAGAGTACTTTTTTT	8	0.2728512960436562	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	6	0.20463847203274216	No Hit
GGTTAATTCCGATAACGAACGAGAC	6	0.20463847203274216	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	6	0.20463847203274216	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG	5	0.17053206002728513	No Hit
TCGTAGTTCCGACCATAAACGATGC	5	0.17053206002728513	No Hit
TTCGTATTGCGCCGCTAGAGGTGAA	4	0.1364256480218281	No Hit
GAACGAGACTCTGGCATGCTAACTA	4	0.1364256480218281	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	4	0.1364256480218281	No Hit
CTCCCGACCCGGGGAGGTAGTGACG	4	0.1364256480218281	No Hit
TATCTAGAGTCACCAAAGCCGCCGG	4	0.1364256480218281	No Hit
GCTTTGAACACTCTAATTTTTTCAA	4	0.1364256480218281	No Hit
CTCCACTCCTGGTGGTGCCCTTCCG	4	0.1364256480218281	No Hit
CTTTAATATACGCTATTGGAGCTGG	4	0.1364256480218281	No Hit
CTCAGCTAAGAGCATCGAGGGGGCG	3	0.10231923601637108	No Hit
GAATAACGCCGCCGCATCGCCAGTC	3	0.10231923601637108	No Hit
ATCAGATACCGTCGTAGTTCCGACC	3	0.10231923601637108	No Hit
GGACTCTTTCGAGGCCCTGTAATTG	3	0.10231923601637108	No Hit
CTCCTGCCCCTCCCAAAGCCGAAGC	3	0.10231923601637108	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	3	0.10231923601637108	No Hit
TGTCAATCCTGTCCGTGTCCGGGCC	3	0.10231923601637108	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	3	0.10231923601637108	No Hit
TAGTTGGTGGAGCGATTTGTCTGGT	3	0.10231923601637108	No Hit
GCCTACCATGGTGACCACGGGTGAC	3	0.10231923601637108	No Hit
CCCCTTGCCTCTCGGCGCCCCCTCG	3	0.10231923601637108	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	3	0.10231923601637108	No Hit
GTACAGTGAAACTGCGAATGGCTCA	3	0.10231923601637108	No Hit
GTCAATCCTGTCCGTGTCCGGGCCG	3	0.10231923601637108	No Hit
GTCCTGTATTGTTATTTTTCGTCAC	3	0.10231923601637108	No Hit
TGTTTTCATTAATCAAGAACGAAAG	3	0.10231923601637108	No Hit
GAATAATGGAATAGGACCGCGGTTC	3	0.10231923601637108	No Hit
GTACATGGGTACCTGGTTGATCCTG	3	0.10231923601637108	No Hit
GTCCTATTCCATTATTCCTAGCTGC	3	0.10231923601637108	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE