##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064167_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2932
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.93212824010914	32.0	32.0	32.0	21.0	32.0
2	29.657571623465213	32.0	32.0	32.0	21.0	32.0
3	30.767394270122782	32.0	32.0	32.0	32.0	32.0
4	31.09038199181446	32.0	32.0	32.0	32.0	32.0
5	30.738403819918144	32.0	32.0	32.0	32.0	32.0
6	34.47646657571624	36.0	36.0	36.0	32.0	36.0
7	34.50989085948158	36.0	36.0	36.0	32.0	36.0
8	34.394270122783084	36.0	36.0	36.0	32.0	36.0
9	34.55798090040928	36.0	36.0	36.0	32.0	36.0
10	34.14085948158254	36.0	36.0	36.0	32.0	36.0
11	34.6643929058663	36.0	36.0	36.0	32.0	36.0
12	34.5	36.0	36.0	36.0	32.0	36.0
13	34.83083219645293	36.0	36.0	36.0	32.0	36.0
14	34.644270122783084	36.0	36.0	36.0	32.0	36.0
15	34.53274215552524	36.0	36.0	36.0	32.0	36.0
16	34.322987721691675	36.0	36.0	36.0	32.0	36.0
17	34.37824010914052	36.0	36.0	36.0	32.0	36.0
18	34.47919508867667	36.0	36.0	36.0	32.0	36.0
19	34.39495225102319	36.0	36.0	36.0	32.0	36.0
20	34.52080491132333	36.0	36.0	36.0	32.0	36.0
21	34.48294679399727	36.0	36.0	36.0	32.0	36.0
22	34.32537517053206	36.0	36.0	36.0	32.0	36.0
23	34.408253751705324	36.0	36.0	36.0	32.0	36.0
24	34.169849931787176	36.0	36.0	36.0	32.0	36.0
25	34.20225102319236	36.0	36.0	36.0	32.0	36.0
26	34.09515688949522	36.0	36.0	36.0	32.0	36.0
27	34.125170532060025	36.0	36.0	36.0	32.0	36.0
28	34.13608458390178	36.0	36.0	36.0	32.0	36.0
29	34.0668485675307	36.0	36.0	36.0	32.0	36.0
30	34.01364256480218	36.0	36.0	36.0	32.0	36.0
31	33.99795361527967	36.0	36.0	36.0	32.0	36.0
32	33.88301500682128	36.0	36.0	36.0	32.0	36.0
33	33.94338335607094	36.0	36.0	36.0	32.0	36.0
34	34.02319236016371	36.0	36.0	36.0	32.0	36.0
35	33.929740791268756	36.0	36.0	36.0	32.0	36.0
36	33.76023192360164	36.0	36.0	36.0	32.0	36.0
37	33.94031377899045	36.0	36.0	36.0	32.0	36.0
38	33.88574351978172	36.0	36.0	36.0	32.0	36.0
39	33.777285129604365	36.0	36.0	36.0	32.0	36.0
40	33.61425648021828	36.0	36.0	36.0	27.0	36.0
41	33.68519781718963	36.0	36.0	36.0	27.0	36.0
42	33.632332878581174	36.0	36.0	36.0	27.0	36.0
43	33.8918826739427	36.0	36.0	36.0	32.0	36.0
44	33.510914051841745	36.0	36.0	36.0	21.0	36.0
45	33.427353342428376	36.0	36.0	36.0	21.0	36.0
46	33.69542974079127	36.0	36.0	36.0	27.0	36.0
47	33.48772169167803	36.0	36.0	36.0	21.0	36.0
48	33.3581173260573	36.0	36.0	36.0	21.0	36.0
49	33.49045020463847	36.0	36.0	36.0	21.0	36.0
50	32.438949522510235	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	2.0
23	3.0
24	4.0
25	11.0
26	15.0
27	26.0
28	63.0
29	64.0
30	141.0
31	179.0
32	303.0
33	440.0
34	612.0
35	1069.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.01909959072306	19.167803547066846	11.732605729877218	27.080491132332877
2	17.66712141882674	20.770804911323328	36.59618008185539	24.965893587994543
3	21.077762619372443	23.15825375170532	28.206002728512956	27.55798090040928
4	14.529331514324692	16.882673942701228	34.618008185538876	33.969986357435204
5	18.11050477489768	33.049113233287855	31.71896316507503	17.121418826739426
6	34.65211459754434	35.91405184174625	15.34788540245566	14.085948158253753
7	31.582537517053204	28.990450204638474	18.963165075034105	20.463847203274216
8	27.83083219645293	31.309686221009546	19.47476125511596	21.384720327421554
9	27.182810368349248	13.676671214188268	17.7012278308322	41.439290586630285
10	16.132332878581174	25.4774897680764	31.889495225102323	26.50068212824011
11	38.33560709413369	19.679399727148706	22.919508867667123	19.065484311050476
12	24.79536152796726	22.85129604365621	28.30832196452933	24.0450204638472
13	30.72987721691678	18.21282401091405	24.69304229195089	26.36425648021828
14	24.01091405184175	17.66712141882674	24.727148703956345	33.59481582537517
15	25.57980900409277	26.26193724420191	21.725784447476126	26.432469304229194
16	26.773533424283762	25.648021828103683	22.510231923601637	25.068212824010917
17	24.11323328785812	25.51159618008186	24.89768076398363	25.4774897680764
18	25.613915416098227	24.829467939972716	25.954979536152795	23.601637107776263
19	25.068212824010917	23.703956343792633	25.852660300136428	25.375170532060025
20	25.852660300136428	23.976807639836288	25.57980900409277	24.590723055934514
21	24.727148703956345	24.727148703956345	25.306957708049115	25.2387448840382
22	25.443383356070942	24.21555252387449	25.818553888130968	24.522510231923604
23	23.94270122783083	24.7612551159618	25.648021828103683	25.648021828103683
24	25.613915416098227	24.181446111869032	24.863574351978173	25.34106412005457
25	23.94270122783083	25.170532060027284	25.4774897680764	25.409276944065486
26	25.0	25.375170532060025	23.94270122783083	25.682128240109144
27	24.7612551159618	24.590723055934514	25.34106412005457	25.306957708049115
28	23.806275579809004	24.863574351978173	25.818553888130968	25.51159618008186
29	24.931787175989086	24.31787175989086	25.750341064120054	25.0
30	25.443383356070942	25.34106412005457	25.102319236016374	24.11323328785812
31	25.136425648021827	25.170532060027284	24.21555252387449	25.4774897680764
32	24.965893587994543	24.386084583901773	25.306957708049115	25.34106412005457
33	23.94270122783083	24.0450204638472	26.125511596180083	25.886766712141885
34	26.091405184174626	23.73806275579809	25.034106412005457	25.136425648021827
35	27.59208731241473	23.669849931787176	24.01091405184175	24.727148703956345
36	25.306957708049115	25.068212824010917	23.874488403819917	25.750341064120054
37	25.750341064120054	24.829467939972716	23.908594815825378	25.51159618008186
38	25.375170532060025	25.34106412005457	23.362892223738065	25.92087312414734
39	25.34106412005457	24.181446111869032	24.01091405184175	26.46657571623465
40	25.34106412005457	25.306957708049115	24.181446111869032	25.170532060027284
41	25.54570259208731	24.829467939972716	24.181446111869032	25.443383356070942
42	26.773533424283762	25.57980900409277	24.249658935879946	23.396998635743522
43	24.7612551159618	24.386084583901773	25.613915416098227	25.2387448840382
44	24.829467939972716	24.454297407912687	24.522510231923604	26.193724420190996
45	25.443383356070942	24.31787175989086	24.283765347885403	25.954979536152795
46	25.375170532060025	23.53342428376535	25.034106412005457	26.05729877216917
47	26.227830832196453	23.94270122783083	25.443383356070942	24.386084583901773
48	25.375170532060025	26.56889495225102	23.63574351978172	24.42019099590723
49	23.806275579809004	25.20463847203274	26.80763983628922	24.181446111869032
50	26.46657571623465	23.53342428376535	23.772169167803547	26.227830832196453
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	1.0
10	1.0
11	1.5
12	2.0
13	1.5
14	1.0
15	5.0
16	9.0
17	5.5
18	2.0
19	4.5
20	7.0
21	13.0
22	19.0
23	15.0
24	11.0
25	19.0
26	27.0
27	27.5
28	28.0
29	36.0
30	44.0
31	47.0
32	50.0
33	59.0
34	68.0
35	77.0
36	86.0
37	88.0
38	90.0
39	104.5
40	119.0
41	117.5
42	116.0
43	132.0
44	148.0
45	154.0
46	160.0
47	184.0
48	208.0
49	218.0
50	228.0
51	219.5
52	211.0
53	206.0
54	201.0
55	202.5
56	204.0
57	193.5
58	183.0
59	174.5
60	166.0
61	153.5
62	141.0
63	132.5
64	124.0
65	101.5
66	79.0
67	72.5
68	66.0
69	59.0
70	52.0
71	36.0
72	20.0
73	19.5
74	19.0
75	16.5
76	14.0
77	9.0
78	4.0
79	5.0
80	6.0
81	4.0
82	2.0
83	3.0
84	4.0
85	2.5
86	1.0
87	1.0
88	1.0
89	1.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	3.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2932.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.36152796725784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.7453505007153	92.25784447476126
2	2.3605150214592276	4.502046384720328
3	0.5007153075822603	1.4324693042291952
4	0.17882689556509299	0.6821282401091405
5	0.1072961373390558	0.5115961800818555
6	0.1072961373390558	0.6139154160982265
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	6	0.20463847203274216	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	6	0.20463847203274216	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	6	0.20463847203274216	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	5	0.17053206002728513	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC	5	0.17053206002728513	No Hit
AATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCA	5	0.17053206002728513	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTA	4	0.1364256480218281	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	4	0.1364256480218281	No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGA	4	0.1364256480218281	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	4	0.1364256480218281	No Hit
TTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATG	4	0.1364256480218281	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	3	0.10231923601637108	No Hit
GGTCTACAGAGTGAGTCCAGGACAGCCAGGGCTATACAGAGAACCCTGTC	3	0.10231923601637108	No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT	3	0.10231923601637108	No Hit
CCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGG	3	0.10231923601637108	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	3	0.10231923601637108	No Hit
TATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATG	3	0.10231923601637108	No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	3	0.10231923601637108	No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG	3	0.10231923601637108	No Hit
ACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAG	3	0.10231923601637108	No Hit
TCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAA	3	0.10231923601637108	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	3	0.10231923601637108	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT	3	0.10231923601637108	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTAC	3	0.10231923601637108	No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG	3	0.10231923601637108	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.06821282401091405	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE