FastQCFastQC Report
Thu 2 Feb 2017
SRR4064166_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064166_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences625026
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30700.4911795669300157No Hit
GTACTTTTTTTTTTTTTTTTTTTTT24900.39838342724942644No Hit
TATCAACGCAGAGTACTTTTTTTTT22990.36782469849254273No Hit
GAGTACTTTTTTTTTTTTTTTTTTT17880.28606809956705803No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13580.21727096152800046No Hit
GTACATGGGGTGGTATCAACGCAAA11510.18415233926268668No Hit
GGTATCAACGCAGAGTACTTTTTTT10620.16991293162204452No Hit
GTATCAACGCAGAGTACATGGGGTG8510.13615433597962326No Hit
GTACATGGGTGGTATCAACGCAAAA6760.10815550073116958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA654.8567017E-1019.00088110
CCGTCGT555.8928345E-717.2735279
TACCGTC601.4626767E-615.8340677
ATACCGT704.4253284E-714.9280686
CACTCTA1058.731149E-1114.4768619
ATCGAAC400.00527280314.2506618
GTCTTAC400.00527840914.2483781
TTATACT400.00528682714.2449574
AACACTC1101.8553692E-1013.8188227
ACTCTAA1051.3624231E-913.57205710
GTATATG655.4481192E-513.1523491
TTGAACA1301.4551915E-1113.1491924
TACGAGC801.989325E-613.06519714
GTATTAG801.9964955E-613.0610131
ACCGTCG751.47452865E-512.6672548
ACAATAC751.481831E-512.6611713
GTAGGAC2000.012.3446413
TCTAATT1251.3988029E-912.16153712
CTAATTT1251.3988029E-912.16153713
TTTGAAC1251.4115358E-912.1547243
Produced by FastQC (version 0.11.5)