##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064166_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	625026
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.176901440900057	32.0	32.0	32.0	32.0	32.0
2	30.82745357793116	32.0	32.0	32.0	32.0	32.0
3	30.84630399375386	32.0	32.0	32.0	32.0	32.0
4	30.856073187355406	32.0	32.0	32.0	32.0	32.0
5	30.798432385212774	32.0	32.0	32.0	32.0	32.0
6	34.42794059767114	36.0	36.0	36.0	32.0	36.0
7	34.34366250363985	36.0	36.0	36.0	32.0	36.0
8	34.30093148125038	36.0	36.0	36.0	32.0	36.0
9	34.4049927523015	36.0	36.0	36.0	32.0	36.0
10	34.14731227180949	36.0	36.0	36.0	32.0	36.0
11	34.4316988413282	36.0	36.0	36.0	32.0	36.0
12	34.25214311084659	36.0	36.0	36.0	32.0	36.0
13	34.33162780428334	36.0	36.0	36.0	32.0	36.0
14	34.234150259349214	36.0	36.0	36.0	32.0	36.0
15	34.191854418856174	36.0	36.0	36.0	32.0	36.0
16	34.17734622239715	36.0	36.0	36.0	32.0	36.0
17	34.10207255378176	36.0	36.0	36.0	32.0	36.0
18	34.09718155724722	36.0	36.0	36.0	32.0	36.0
19	34.1147968244521	36.0	36.0	36.0	32.0	36.0
20	34.08023506222141	36.0	36.0	36.0	32.0	36.0
21	34.05082668560988	36.0	36.0	36.0	32.0	36.0
22	34.05418654583969	36.0	36.0	36.0	32.0	36.0
23	33.995763376243545	36.0	36.0	36.0	32.0	36.0
24	34.001788725589016	36.0	36.0	36.0	32.0	36.0
25	33.611486562159016	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	3.0
5	6.0
6	30.0
7	11.0
8	30.0
9	39.0
10	47.0
11	23.0
12	36.0
13	30.0
14	78.0
15	135.0
16	206.0
17	245.0
18	348.0
19	475.0
20	660.0
21	985.0
22	1571.0
23	2381.0
24	3545.0
25	5062.0
26	7197.0
27	9769.0
28	12853.0
29	17128.0
30	22598.0
31	30514.0
32	42622.0
33	60087.0
34	128058.0
35	278253.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.099562083050564	16.902057697478774	11.38071884963571	25.61766136983495
2	16.224981719795263	20.397988457470724	38.03226596137877	25.344763861355247
3	19.23442449841605	24.694729768647402	28.44116988256376	27.629675850372788
4	12.580718450205755	15.614900193916275	35.87819113873014	35.92619021714783
5	14.259450083844294	37.185583901895775	33.425711396423495	15.129254617836432
6	33.09288512250698	35.479178934432525	17.488927007859903	13.939008935200595
7	29.273764830515255	30.98189452931217	21.2280802824159	18.516260357756675
8	27.80147337726044	32.85666500753013	20.033993626995166	19.307867988214262
9	27.714042863797232	14.15587388330508	18.891892886927486	39.2381903659702
10	15.80735219727094	27.15901159626307	32.20140102835994	24.83223517810605
11	36.443981283606504	21.361372706273322	23.089901759744386	19.104744250375784
12	24.92964240089904	23.814166290465625	29.60157908386242	21.65461222477292
13	29.6729615327967	19.990682573530822	25.513899294949375	24.822456598723097
14	23.39032707362759	19.820213566431327	26.323103277140063	30.466356082801017
15	24.558002382080605	27.50102455079851	23.57875593919291	24.362217127927973
16	24.51571485290539	25.95576275310903	25.118950037782245	24.40957235620333
17	23.1230659138172	26.15885081061524	26.17293872238649	24.545144553181068
18	23.80273053171237	25.199538240092735	27.6345051851923	23.36322604300259
19	24.872034211735546	24.8561835854757	26.768185391486455	23.503596811302295
20	25.519895988779083	24.427265798625566	26.299820029845584	23.753018182749763
21	26.14170530522325	24.16665599303225	25.584061275200447	24.107577426544047
22	25.233803475389994	24.810947814712048	25.989691992641255	23.965556717256707
23	23.935881485466695	24.784998662996713	26.469142444490345	24.809977407046247
24	24.062397930102534	25.2779503403931	26.67394631843887	23.985705411065496
25	24.350075971904005	24.888683948873872	26.445907844239187	24.31533223498294
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	505.0
1	505.0
2	612.0
3	719.0
4	719.0
5	719.0
6	1878.0
7	3037.0
8	3037.0
9	3037.0
10	2942.5
11	2848.0
12	2848.0
13	2848.0
14	2400.5
15	1953.0
16	1953.0
17	1953.0
18	3350.0
19	4747.0
20	4747.0
21	4747.0
22	7685.0
23	10623.0
24	10623.0
25	10623.0
26	15341.0
27	20059.0
28	20059.0
29	20059.0
30	25144.5
31	30230.0
32	30230.0
33	30230.0
34	35101.0
35	39972.0
36	39972.0
37	39972.0
38	45447.5
39	50923.0
40	50923.0
41	50923.0
42	58228.0
43	65533.0
44	65533.0
45	65533.0
46	73772.5
47	82012.0
48	82012.0
49	82012.0
50	84196.0
51	86380.0
52	86380.0
53	86380.0
54	78810.5
55	71241.0
56	71241.0
57	71241.0
58	64625.0
59	58009.0
60	58009.0
61	58009.0
62	50102.5
63	42196.0
64	42196.0
65	42196.0
66	34591.5
67	26987.0
68	26987.0
69	26987.0
70	20332.0
71	13677.0
72	13677.0
73	13677.0
74	10111.0
75	6545.0
76	6545.0
77	6545.0
78	5189.5
79	3834.0
80	3834.0
81	3834.0
82	2640.5
83	1447.0
84	1447.0
85	1447.0
86	1112.0
87	777.0
88	777.0
89	777.0
90	537.5
91	298.0
92	298.0
93	298.0
94	209.0
95	120.0
96	120.0
97	120.0
98	237.0
99	354.0
100	354.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03999833606921952
2	0.005279780361136977
3	9.599600656612686E-4
4	0.002239906819876293
5	0.009279613968058929
6	0.013119454230704002
7	0.021119121444547907
8	0.03247864888820625
9	0.048477983315894056
10	0.0547177237426923
11	0.0532777836442004
12	0.05759760393967611
13	0.06239740426798245
14	0.06335736433364372
15	0.05791759062822987
16	0.06303737764508996
17	0.060317490792383036
18	0.07343694502308704
19	0.07119703820321074
20	0.07647681856434772
21	0.06943711141616508
22	0.07407691840019455
23	0.07919670541705465
24	0.07263697830170265
25	0.07247698495742577
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	625026.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.602043653765826
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.50280579719036	50.39640162766344
2	12.66151788670434	15.852737909328038
3	3.4028391947681564	6.390740634528644
4	1.2208617477390042	3.0571376170867994
5	0.6233612324161096	1.9511843541889278
6	0.35278236129853835	1.325093806937381
7	0.22537730421916696	0.9876355886107446
8	0.1676551915896731	0.839644609814175
9	0.11283604211187077	0.6357390150604945
>10	0.604802117224769	6.972370425815696
>50	0.07144724546146797	3.164545747149484
>100	0.050886832998831434	5.866045239859854
>500	0.001028016828256129	0.43408091215345207
>1k	0.001799029449448226	2.1266425118029386
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3070	0.4911795669300157	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2490	0.39838342724942644	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2299	0.36782469849254273	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1788	0.28606809956705803	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1358	0.21727096152800046	No Hit
GTACATGGGGTGGTATCAACGCAAA	1151	0.18415233926268668	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1062	0.16991293162204452	No Hit
GTATCAACGCAGAGTACATGGGGTG	851	0.13615433597962326	No Hit
GTACATGGGTGGTATCAACGCAAAA	676	0.10815550073116958	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	1.599933442768781E-4
5	0.0	0.0	0.0	0.0	1.599933442768781E-4
6	0.0	0.0	0.0	0.0	1.599933442768781E-4
7	0.0	0.0	0.0	0.0	1.599933442768781E-4
8	0.0	0.0	0.0	0.0	1.599933442768781E-4
9	0.0	0.0	0.0	0.0	1.599933442768781E-4
10	0.0	0.0	0.0	0.0	1.599933442768781E-4
11	0.0	0.0	0.0	0.0	1.599933442768781E-4
12	0.0	0.0	0.0	0.0	1.599933442768781E-4
13	0.0	0.0	0.0	0.0	3.199866885537562E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCGTA	65	4.8567017E-10	19.000881	10
CCGTCGT	55	5.8928345E-7	17.273527	9
TACCGTC	60	1.4626767E-6	15.834067	7
ATACCGT	70	4.4253284E-7	14.928068	6
CACTCTA	105	8.731149E-11	14.476861	9
ATCGAAC	40	0.005272803	14.250661	8
GTCTTAC	40	0.005278409	14.248378	1
TTATACT	40	0.005286827	14.244957	4
AACACTC	110	1.8553692E-10	13.818822	7
ACTCTAA	105	1.3624231E-9	13.572057	10
GTATATG	65	5.4481192E-5	13.152349	1
TTGAACA	130	1.4551915E-11	13.149192	4
TACGAGC	80	1.989325E-6	13.065197	14
GTATTAG	80	1.9964955E-6	13.061013	1
ACCGTCG	75	1.47452865E-5	12.667254	8
ACAATAC	75	1.481831E-5	12.661171	3
GTAGGAC	200	0.0	12.344641	3
TCTAATT	125	1.3988029E-9	12.161537	12
CTAATTT	125	1.3988029E-9	12.161537	13
TTTGAAC	125	1.4115358E-9	12.154724	3
>>END_MODULE