FastQCFastQC Report
Thu 2 Feb 2017
SRR4064166_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064166_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences625026
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9830.15727345742417115No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA8210.13135453565131688No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGACG703.213548E-521.998763
ATACCGT1057.735798E-820.95126
GCGAAGC550.004480617620.00047326
TAATACC550.004482692619.9988734
CGAAAAC901.00048655E-519.55601923
CCGTCGT901.0011781E-519.5544559
CGTCGTA901.0011781E-519.55445510
ACCGTCG901.0011781E-519.5544558
GTCCTAT1352.59206E-919.5544531
CGGTCCG808.978094E-519.25045626
ACTGATC708.120405E-418.856088
ACGGACC2000.018.6989468
TACCGTC951.595873E-518.5252727
CGGACCA2150.018.4175669
GTATTAT600.007410605418.33231
ATAGATC600.007410605418.33233
TCTGTAC600.007410605418.33233
TATAGGG600.007410605418.33235
GTATAGA1203.1577656E-718.33231
GATTTCG1701.2551027E-1018.11807441
Produced by FastQC (version 0.11.5)