##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064166_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	625026
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.213823104958834	32.0	32.0	32.0	32.0	32.0
2	31.401935279492374	32.0	32.0	32.0	32.0	32.0
3	31.474721051604252	32.0	32.0	32.0	32.0	32.0
4	31.561522240674787	32.0	32.0	32.0	32.0	32.0
5	31.507590084252495	32.0	32.0	32.0	32.0	32.0
6	35.07093784898548	36.0	36.0	36.0	36.0	36.0
7	35.10126618732661	36.0	36.0	36.0	36.0	36.0
8	35.050562696591825	36.0	36.0	36.0	36.0	36.0
9	35.150785727313746	36.0	36.0	36.0	36.0	36.0
10	35.013399442583186	36.0	36.0	36.0	36.0	36.0
11	35.15712786348088	36.0	36.0	36.0	36.0	36.0
12	35.06481330376656	36.0	36.0	36.0	36.0	36.0
13	35.1016437716191	36.0	36.0	36.0	36.0	36.0
14	35.04108469087686	36.0	36.0	36.0	36.0	36.0
15	35.02160710114459	36.0	36.0	36.0	36.0	36.0
16	35.022621458947306	36.0	36.0	36.0	36.0	36.0
17	34.99946722216356	36.0	36.0	36.0	36.0	36.0
18	34.990077212787945	36.0	36.0	36.0	36.0	36.0
19	34.983943067968376	36.0	36.0	36.0	36.0	36.0
20	34.96802053034594	36.0	36.0	36.0	36.0	36.0
21	34.970636421524866	36.0	36.0	36.0	36.0	36.0
22	34.95746416949055	36.0	36.0	36.0	36.0	36.0
23	34.9065750864764	36.0	36.0	36.0	32.0	36.0
24	34.8854575649653	36.0	36.0	36.0	32.0	36.0
25	34.85718994089846	36.0	36.0	36.0	32.0	36.0
26	34.80792959012905	36.0	36.0	36.0	32.0	36.0
27	34.78815601270987	36.0	36.0	36.0	32.0	36.0
28	34.76275386943903	36.0	36.0	36.0	32.0	36.0
29	34.7377261105938	36.0	36.0	36.0	32.0	36.0
30	34.714344683261174	36.0	36.0	36.0	32.0	36.0
31	34.688929740522795	36.0	36.0	36.0	32.0	36.0
32	34.64089973857087	36.0	36.0	36.0	32.0	36.0
33	34.58802833802114	36.0	36.0	36.0	32.0	36.0
34	34.57282097064762	36.0	36.0	36.0	32.0	36.0
35	34.51511617116728	36.0	36.0	36.0	32.0	36.0
36	34.47540422318431	36.0	36.0	36.0	32.0	36.0
37	34.46646059523924	36.0	36.0	36.0	32.0	36.0
38	34.4083894110005	36.0	36.0	36.0	32.0	36.0
39	34.379608208298535	36.0	36.0	36.0	32.0	36.0
40	34.358473087519556	36.0	36.0	36.0	32.0	36.0
41	34.334130100187835	36.0	36.0	36.0	32.0	36.0
42	34.255011791509475	36.0	36.0	36.0	32.0	36.0
43	34.23048161196494	36.0	36.0	36.0	32.0	36.0
44	34.14941298441985	36.0	36.0	36.0	32.0	36.0
45	34.09476085794831	36.0	36.0	36.0	32.0	36.0
46	34.04210224854646	36.0	36.0	36.0	32.0	36.0
47	33.98866927135831	36.0	36.0	36.0	32.0	36.0
48	33.91419396953087	36.0	36.0	36.0	32.0	36.0
49	33.882267297680414	36.0	36.0	36.0	32.0	36.0
50	33.301849843046526	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	9.0
21	16.0
22	74.0
23	232.0
24	604.0
25	1390.0
26	2953.0
27	5274.0
28	8477.0
29	12604.0
30	17490.0
31	23961.0
32	33562.0
33	51962.0
34	103982.0
35	362431.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.199703024794864	17.895636256576335	11.845886509389281	26.058774209239516
2	15.591856325746969	21.09275628974841	37.55961761529539	25.75576976920923
3	18.410522492772266	25.331387804928777	28.760381617956458	27.4977080843425
4	12.242850697410988	16.179486933343572	35.86298809969505	35.714674269550386
5	13.846623980442413	37.50643973210715	33.31749399224992	15.32944229520052
6	33.261229550817966	35.720651173953044	17.245390184392626	13.772729090836366
7	29.264429422823085	31.156033758649652	21.212271509139637	18.367265309387623
8	27.94379753802242	32.92791019893572	19.848614297645216	19.279677965396637
9	27.743707811417533	13.99760968031474	18.83952699189307	39.41915551637465
10	15.720296950497584	27.381363796358514	32.16009087709193	24.738248376051967
11	36.53911997260914	21.21575742449114	23.17199604496453	19.07312655793519
12	24.973521101522174	23.83500846364791	29.60068861135377	21.590781823476142
13	29.867220886271383	19.96822516931377	25.569459043907184	24.59509490050766
14	23.476783365811983	19.760457964948657	26.44321996204958	30.319538707189782
15	24.607761255163986	27.495432014617556	23.54888464356914	24.347922086649323
16	24.583895312652494	26.001443146892196	25.053318037896204	24.361343502559105
17	23.276791685465884	26.02387740669988	26.245468188523358	24.453862719310877
18	23.985760569577216	25.097076116955318	27.64657413703452	23.270589176432942
19	25.007439690508875	24.772729454454694	26.897441066451638	23.322389788584793
20	25.645140387732212	24.358459502831113	26.415516968284162	23.58088314115251
21	26.125153193627142	24.128116270363154	25.84548482783116	23.90124570817854
22	25.21479106469171	24.638495038606393	26.275706930591685	23.87100696611021
23	24.06003007903747	24.66337077213529	26.59242904227065	24.684170106556593
24	24.263465925999405	25.01719939458131	26.81649605933871	23.902838620080573
25	24.439694343824797	24.66688852765369	26.73787551038693	24.155541618134578
26	23.844228904597482	25.42904411882354	27.062339798462066	23.66438717811691
27	24.272946040563976	25.203523907659026	26.629951295064863	23.893578756712127
28	23.832571207316413	25.227757813524992	26.894781810094624	24.04488916906398
29	23.829348095645706	25.158116205211073	26.715998816028414	24.296536883114804
30	23.69012628739382	25.44099788321016	26.92147077694027	23.947405052455746
31	24.258977472915177	25.178685432037135	26.177435794048137	24.38490130099955
32	23.94217015516651	25.520235005743945	26.016550241117685	24.52104459797186
33	23.668686390305325	25.241638840133568	26.677791946598656	24.41188282296245
34	24.313652079723397	25.39475305234628	26.505453504018327	23.786141363911995
35	24.607084400654713	25.3945955805295	26.492343285755197	23.505976733060592
36	23.952324798113217	25.943431699305098	26.26376246241874	23.84048104016295
37	24.334607452396533	25.94930936773508	25.981629005755135	23.73445417411325
38	24.06519961662344	25.933440324622026	26.084326842919616	23.917033215834923
39	24.430001823994164	25.527918310661406	26.001516795146255	24.040563070198175
40	24.74020623505036	25.79474092621777	26.197771253489915	23.267281585241953
41	23.887374241609706	25.843829198986253	26.618539282697178	23.650257276706856
42	24.53956810360138	26.30497703240423	26.019542056059812	23.13591280793458
43	24.010905320823785	25.686222432705723	26.150850538226223	24.15202170824427
44	23.583480795753083	26.275563263778	25.676220524848887	24.46473541562003
45	23.74887803410776	26.42927654999288	25.73441767370272	24.08742774219664
46	23.761121626313354	25.86857081602058	25.666977589552992	24.70332996811307
47	23.93673020739001	25.894791410002465	25.917351193428544	24.251127189178984
48	24.276615046407034	26.201695004743904	25.23243516534856	24.289254783500503
49	23.925122995080198	26.383264669413226	25.324587016519338	24.36702531898724
50	23.97871431519901	26.69414307679262	25.09147692637527	24.235665681633098
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	280.0
1	168.5
2	57.0
3	214.0
4	371.0
5	380.0
6	389.0
7	341.5
8	294.0
9	428.0
10	562.0
11	813.5
12	1065.0
13	1760.0
14	2455.0
15	3132.5
16	3810.0
17	3942.0
18	4074.0
19	4000.0
20	3926.0
21	4128.0
22	4330.0
23	4678.5
24	5027.0
25	4917.5
26	4808.0
27	5683.0
28	6558.0
29	7722.0
30	8886.0
31	10400.5
32	11915.0
33	14026.5
34	16138.0
35	18326.5
36	20515.0
37	22408.5
38	24302.0
39	25695.0
40	27088.0
41	28850.0
42	30612.0
43	31952.5
44	33293.0
45	36364.0
46	39435.0
47	41584.0
48	43733.0
49	45358.0
50	46983.0
51	45873.0
52	44763.0
53	44563.0
54	44363.0
55	43778.0
56	43193.0
57	41503.0
58	39813.0
59	36293.5
60	32774.0
61	28645.0
62	24516.0
63	21117.0
64	17718.0
65	14994.0
66	12270.0
67	10413.0
68	8556.0
69	7401.5
70	6247.0
71	4928.5
72	3610.0
73	3046.0
74	2482.0
75	1959.0
76	1436.0
77	1207.5
78	979.0
79	788.0
80	597.0
81	473.0
82	349.0
83	293.5
84	238.0
85	167.5
86	97.0
87	71.5
88	46.0
89	35.0
90	24.0
91	21.0
92	18.0
93	15.5
94	13.0
95	10.5
96	8.0
97	6.5
98	5.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009279613968058929
2	1.5999334427687807E-4
3	4.799800328306343E-4
4	0.0
5	0.0
6	1.5999334427687807E-4
7	1.5999334427687807E-4
8	0.0
9	7.999667213843903E-4
10	9.599600656612686E-4
11	0.0
12	0.0
13	4.799800328306343E-4
14	0.0
15	0.0038398402626450743
16	4.799800328306343E-4
17	0.0
18	1.5999334427687807E-4
19	0.0
20	0.0011199534099381465
21	0.0
22	0.0
23	9.599600656612686E-4
24	6.399733771075123E-4
25	0.0015999334427687806
26	0.005119787016860098
27	0.006079747082521367
28	0.0031998668855375612
29	0.0017599267870456589
30	0.003679846918368196
31	0.007199700492459514
32	0.0031998668855375612
33	0.003679846918368196
34	0.0023999001641531715
35	0.0030398735412606836
36	0.00751968718101327
37	0.0030398735412606836
38	0.008479647246674538
39	0.0038398402626450743
40	0.0017599267870456589
41	0.0028798801969838056
42	0.0023999001641531715
43	0.0015999334427687806
44	0.0012799467542150245
45	0.0011199534099381465
46	7.999667213843903E-4
47	0.0031998668855375612
48	0.002079913475599415
49	1.5999334427687807E-4
50	7.999667213843903E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	625026.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.41697828432281
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.82520564895115	55.16408112345588
2	12.0836233659636	16.29282748118207
3	3.110165062489256	6.290337914354928
4	1.1157670337725836	3.008865675448381
5	0.5400062285476195	1.820279409169696
6	0.3151265476036302	1.2746927769964531
7	0.18464186015520928	0.8713597393522498
8	0.12378705040538124	0.6676279111247967
9	0.09741506343341046	0.5910686294450544
>10	0.5059963047223066	6.172927562871118
>50	0.060250642854233795	2.8659059720218507
>100	0.03681974484062031	4.421513718461507
>500	0.001195446261029867	0.5585120861160833
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	983	0.15727345742417115	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	821	0.13135453565131688	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.599933442768781E-4	0.0
11	0.0	0.0	0.0	1.599933442768781E-4	0.0
12	0.0	0.0	0.0	1.599933442768781E-4	0.0
13	0.0	0.0	0.0	1.599933442768781E-4	1.599933442768781E-4
14	0.0	0.0	0.0	1.599933442768781E-4	1.599933442768781E-4
15	0.0	0.0	0.0	1.599933442768781E-4	1.599933442768781E-4
16	0.0	0.0	0.0	1.599933442768781E-4	1.599933442768781E-4
17	0.0	0.0	0.0	3.199866885537562E-4	1.599933442768781E-4
18	0.0	0.0	0.0	3.199866885537562E-4	4.7998003283063423E-4
19	0.0	0.0	0.0	3.199866885537562E-4	4.7998003283063423E-4
20	0.0	0.0	0.0	3.199866885537562E-4	4.7998003283063423E-4
21	0.0	0.0	0.0	4.7998003283063423E-4	6.399733771075124E-4
22	0.0	0.0	0.0	9.599600656612685E-4	7.999667213843904E-4
23	0.0	0.0	0.0	0.002239906819876293	7.999667213843904E-4
24	0.0	0.0	0.0	0.003839840262645074	7.999667213843904E-4
25	0.0	0.0	0.0	0.005439773705413855	7.999667213843904E-4
26	0.0	0.0	0.0	0.0070397071481826355	7.999667213843904E-4
27	0.0	0.0	0.0	0.009439607312335807	7.999667213843904E-4
28	0.0	0.0	0.0	0.01583934108341093	7.999667213843904E-4
29	0.0	0.0	0.0	0.02607891511713113	7.999667213843904E-4
30	0.0	0.0	0.0	0.04607808315174089	7.999667213843904E-4
31	0.0	0.0	0.0	0.08527645249957602	0.0011199534099381465
32	0.0	0.0	0.0	0.1270347153558412	0.0011199534099381465
33	0.0	0.0	0.0	0.1663930780479532	0.0011199534099381465
34	0.0	0.0	0.0	0.20831133424849527	0.0012799467542150248
35	0.0	0.0	0.0	0.25438941740023613	0.0012799467542150248
36	0.0	0.0	0.0	0.3204666685865868	0.0012799467542150248
37	0.0	0.0	0.0	0.4201425220710818	0.0012799467542150248
38	0.0	0.0	0.0	0.5588567515591352	0.0014399400984919028
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGACG	70	3.213548E-5	21.99876	3
ATACCGT	105	7.735798E-8	20.9512	6
GCGAAGC	55	0.0044806176	20.000473	26
TAATACC	55	0.0044826926	19.998873	4
CGAAAAC	90	1.00048655E-5	19.556019	23
CCGTCGT	90	1.0011781E-5	19.554455	9
CGTCGTA	90	1.0011781E-5	19.554455	10
ACCGTCG	90	1.0011781E-5	19.554455	8
GTCCTAT	135	2.59206E-9	19.554453	1
CGGTCCG	80	8.978094E-5	19.250456	26
ACTGATC	70	8.120405E-4	18.85608	8
ACGGACC	200	0.0	18.698946	8
TACCGTC	95	1.595873E-5	18.525272	7
CGGACCA	215	0.0	18.417566	9
GTATTAT	60	0.0074106054	18.3323	1
ATAGATC	60	0.0074106054	18.3323	3
TCTGTAC	60	0.0074106054	18.3323	3
TATAGGG	60	0.0074106054	18.3323	5
GTATAGA	120	3.1577656E-7	18.3323	1
GATTTCG	170	1.2551027E-10	18.118074	41
>>END_MODULE