FastQCFastQC Report
Thu 2 Feb 2017
SRR4064165_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064165_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3028611
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT110690.36548107366710353No Hit
TATCAACGCAGAGTACTTTTTTTTT78900.26051546401964465No Hit
GTACTTTTTTTTTTTTTTTTTTTTT56080.18516739191662449No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG43330.143068885373526No Hit
ACGCAGAGTACTTTTTTTTTTTTTT42280.13960194954056498No Hit
GTATCTGATCGTCTTCGAACCTCCG41750.13785197240583225No Hit
GAATAGGACCGCGGTTCTATTTTGT41730.13778593553282348No Hit
GCGCAAGACGGACCAGAGCGAAAGC41510.13705952992972686No Hit
GATTAAGAGGGACGGCCGGGGGCAT40670.13428598126335803No Hit
GGTATCAACGCAGAGTACTTTTTTT40270.1329652438031824No Hit
GAGTACTTTTTTTTTTTTTTTTTTT40070.13230487507309455No Hit
CTATTGGAGCTGGAATTACCGCGGC37030.12226727037575971No Hit
ATCAGATACCGTCGTAGTTCCGACC36940.12197010444722019No Hit
GTACATGGGGAATAATTGCAATCCC36400.12018710887598308No Hit
GTCTTGCGCCGGTCCAAGAATTTCA34270.11315418190054781No Hit
GAACTACGACGGTATCTGATCGTCT34180.11285701597200828No Hit
GTCCTATTCCATTATTCCTAGCTGC33870.11183344444037216No Hit
TCGTAGTTCCGACCATAAACGATGC33110.10932404326603846No Hit
GAATAACGCCGCCGCATCGCCAGTC30530.1008052866479056No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG655.4610748E-513.1524965
ACGCGCG2400.012.667459513
CGGTCCA7800.012.05844910
GTATTAG6250.011.7020261
CCGTCGT7050.011.5889959
CGCATCG5750.011.56594113
CAACGGA1157.077688E-811.56575114
CGTCGTA7700.011.47474510
CGTTATT3600.011.3439972
GGGTAGG9000.011.2925391
GCGTTAT3750.011.1447881
GTATCAA67750.011.1317011
CGAACGA3000.011.0842116
GTACGTA600.00587582411.08384411
CGGTTCT7600.011.00068912
CGAGCCG8950.010.93400715
ACCGCTA700.001491330310.85782210
TCATATA1408.400093E-910.8540562
ATCGCCA6400.010.83679516
GCGCGCT2900.010.81137915
Produced by FastQC (version 0.11.5)