FastQCFastQC Report
Thu 2 Feb 2017
SRR4064165_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064165_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3028611
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA41610.13738971429477076No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA40830.13481427624742828No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT40460.13359259409676583No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC37570.12405026594699683No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG36770.12140879102664555No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT36710.1212106804076192No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA36260.1197248507649216No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC36010.11889938985231183No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA35820.1182720395587284No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA33170.10952215388506481No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG32260.10651747616316522No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT32090.10595616274259058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAC9450.020.253611
GTATCAA83800.019.8469071
GTCCTAC8250.017.0663661
ATACACT11250.017.0124724
CGTCGTA10100.016.77170410
AACGCAG98150.016.7653266
TCCTACA9050.016.5295492
CGGTTAT800.001988199816.50134332
CGAGCCG15050.016.37180515
CGCCCCT8400.016.2372725
CCTACAG11150.016.1785533
ATACCGT10700.015.6253766
CGCAATA13050.015.51058536
GTATAAA4400.015.4997261
GCTAGGA16250.015.43484835
TGTAGGA10700.015.4197792
TCTAGAT9300.015.37556652
ATACGAA13100.015.28141640
CTAGCGG13600.015.2071229
GTAGGAC10850.015.2066033
Produced by FastQC (version 0.11.5)