##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064165_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3028611
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.139866427216965	32.0	32.0	32.0	32.0	32.0
2	31.14214469933577	32.0	32.0	32.0	32.0	32.0
3	31.309026150931896	32.0	32.0	32.0	32.0	32.0
4	31.44757349161051	32.0	32.0	32.0	32.0	32.0
5	31.313232039373826	32.0	32.0	32.0	32.0	32.0
6	34.91598425813021	36.0	36.0	36.0	36.0	36.0
7	34.94072365186549	36.0	36.0	36.0	36.0	36.0
8	34.872274782070065	36.0	36.0	36.0	32.0	36.0
9	35.04528940824688	36.0	36.0	36.0	36.0	36.0
10	34.82588982209997	36.0	36.0	36.0	32.0	36.0
11	35.04126974378684	36.0	36.0	36.0	36.0	36.0
12	34.909671133070574	36.0	36.0	36.0	32.0	36.0
13	34.98399662419505	36.0	36.0	36.0	36.0	36.0
14	34.90819520895883	36.0	36.0	36.0	32.0	36.0
15	34.85889207957047	36.0	36.0	36.0	32.0	36.0
16	34.87629411634575	36.0	36.0	36.0	32.0	36.0
17	34.82645872976094	36.0	36.0	36.0	32.0	36.0
18	34.834438625495316	36.0	36.0	36.0	32.0	36.0
19	34.833584108358586	36.0	36.0	36.0	32.0	36.0
20	34.82099748036311	36.0	36.0	36.0	32.0	36.0
21	34.81833190198411	36.0	36.0	36.0	32.0	36.0
22	34.798991352801664	36.0	36.0	36.0	32.0	36.0
23	34.7431452900356	36.0	36.0	36.0	32.0	36.0
24	34.704588671176325	36.0	36.0	36.0	32.0	36.0
25	34.67429788771156	36.0	36.0	36.0	32.0	36.0
26	34.60120167297814	36.0	36.0	36.0	32.0	36.0
27	34.6088002057709	36.0	36.0	36.0	32.0	36.0
28	34.57059292196984	36.0	36.0	36.0	32.0	36.0
29	34.537228122066516	36.0	36.0	36.0	32.0	36.0
30	34.51768483968394	36.0	36.0	36.0	32.0	36.0
31	34.51886623934206	36.0	36.0	36.0	32.0	36.0
32	34.45979757717317	36.0	36.0	36.0	32.0	36.0
33	34.42699673216534	36.0	36.0	36.0	32.0	36.0
34	34.41138231354241	36.0	36.0	36.0	32.0	36.0
35	34.361187356184075	36.0	36.0	36.0	32.0	36.0
36	34.32780241503448	36.0	36.0	36.0	32.0	36.0
37	34.32793085675249	36.0	36.0	36.0	32.0	36.0
38	34.26335438919029	36.0	36.0	36.0	32.0	36.0
39	34.263440897493936	36.0	36.0	36.0	32.0	36.0
40	34.238534760654304	36.0	36.0	36.0	32.0	36.0
41	34.21465813866489	36.0	36.0	36.0	32.0	36.0
42	34.12996617921549	36.0	36.0	36.0	32.0	36.0
43	34.120864977377416	36.0	36.0	36.0	32.0	36.0
44	34.034393984569164	36.0	36.0	36.0	32.0	36.0
45	33.995962505584245	36.0	36.0	36.0	32.0	36.0
46	33.96275058104194	36.0	36.0	36.0	32.0	36.0
47	33.93232211069695	36.0	36.0	36.0	32.0	36.0
48	33.87563473816875	36.0	36.0	36.0	32.0	36.0
49	33.84452839932233	36.0	36.0	36.0	32.0	36.0
50	33.13041985253306	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	1.0
17	0.0
18	1.0
19	13.0
20	20.0
21	111.0
22	272.0
23	958.0
24	2494.0
25	5654.0
26	11940.0
27	22209.0
28	38081.0
29	60711.0
30	89646.0
31	133058.0
32	198866.0
33	327794.0
34	652642.0
35	1484139.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.96306799419887	18.485782478793915	12.556135697812953	24.995013829194264
2	15.708804164034815	20.762094445587252	37.63962304707685	25.889478343301082
3	18.10459680888965	24.9370914161357	29.01220728646659	27.946104488508063
4	12.319607899462822	16.2221229467898	36.156673801950795	35.301595351796585
5	14.598045110448322	36.6860583944257	32.560767956003595	16.15512853912239
6	33.67175107739408	35.99532986154677	16.81837572690256	13.514543334156595
7	30.181799571420555	30.8030746778225	20.527139512845828	18.487986237911123
8	28.213701995304774	32.476020352570984	19.457176724636057	19.85310092748819
9	27.690736504548674	14.170124837547416	18.722924569170463	39.416214088733454
10	16.326689641076843	26.138724098069492	30.586474814822886	26.94811144603077
11	37.152687206342186	21.180343457889922	22.776025965707042	18.890943370060853
12	25.386077088296428	23.466414405312015	28.36814308897991	22.779365417411647
13	29.081107388821643	19.352625402743577	25.86295591167146	25.703311296763324
14	24.050859025287863	19.968553199540253	24.973758562930087	31.006829212241797
15	25.05154400887574	27.315451975908704	22.538303043110737	25.094700972104818
16	25.80694369785524	25.802618249824672	23.942543521931672	24.44789453038842
17	23.74266619252192	25.884737260744284	25.23750986838521	25.13508667834859
18	24.81517031472308	24.664638666261197	26.681875018779312	23.83831600023641
19	25.569675339619387	24.46788313190436	25.747644712378047	24.214796816098204
20	25.96572928938596	23.87594631432692	25.118371837474314	25.039952558812807
21	27.532918555733964	23.742996376886964	24.447741885636685	24.27634318174239
22	26.090492367948453	23.939868104466438	25.190376175993666	24.77926335159144
23	24.308670779036817	23.854036173976898	25.724182836478803	26.11311021050748
24	24.73449778775672	24.897048141055272	25.540513768738034	24.827940302449978
25	25.001089625314176	24.142035631408152	25.653808207454095	25.203066535823577
26	24.37516737104216	25.369305958722794	26.269997434331945	23.98552923590311
27	25.122927421061792	24.579969772947084	25.356581153231083	24.940521652760044
28	24.243472874319334	24.729228585853548	26.152815425046978	24.874483114780137
29	24.21094791911215	24.33744455659973	26.019542756424798	25.432064767863316
30	24.103476797958066	25.033044356869148	26.12698344562746	24.73649539954532
31	25.134445262633033	24.632029771791426	24.846831794001393	25.386693171574148
32	24.713423621897487	24.960409600490273	24.73914582474826	25.58702095286398
33	24.29342582399258	24.48144056138942	25.53216359593539	25.692970018682605
34	25.18393387691867	24.585525538098633	25.756620199740205	24.473920385242497
35	25.836736809815747	24.401118565794988	25.59764320604018	24.164501418349086
36	24.449142244271503	25.322582988593194	25.00835433474399	25.21992043239131
37	25.569769384640477	24.94183600758922	25.04789490263516	24.44049970513514
38	24.599658890717897	24.68082506839522	25.46649913897149	25.253016901915398
39	25.4009921782751	24.407922369182245	24.861283914613498	25.329801537929157
40	25.639566021624955	25.01183739480898	25.217316077922746	24.131280505643318
41	24.257200980944102	25.29959442115284	26.084699708866832	24.35850488903623
42	25.97721552925918	25.571576402467976	25.017780762700614	23.43342730557223
43	24.943694679052243	24.359922168067712	25.49494366496213	25.20143948791792
44	24.429285108153355	25.126247701060233	24.89178060933048	25.552686581455937
45	24.639418711462987	25.412585823992877	24.97502131360485	24.972974150939283
46	24.50262945270997	24.92328905621353	24.938180538252784	25.63590095282372
47	24.817899053669425	24.901503694849662	25.55485481453109	24.72574243694982
48	25.709035677139227	25.382344686401083	24.225388387181983	24.683231249277707
49	24.70801690550089	25.58720200752823	24.632767615399857	25.072013471571026
50	24.831686019059845	25.669871483731903	24.358722907144834	25.139719590063415
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	108.0
1	90.0
2	72.0
3	228.0
4	384.0
5	511.0
6	638.0
7	623.5
8	609.0
9	829.0
10	1049.0
11	1559.0
12	2069.0
13	3724.5
14	5380.0
15	7333.0
16	9286.0
17	9996.5
18	10707.0
19	10839.0
20	10971.0
21	11889.0
22	12807.0
23	14345.5
24	15884.0
25	17291.0
26	18698.0
27	23554.0
28	28410.0
29	33320.0
30	38230.0
31	43258.5
32	48287.0
33	56716.5
34	65146.0
35	75894.0
36	86642.0
37	100733.5
38	114825.0
39	125225.0
40	135625.0
41	145397.0
42	155169.0
43	157113.5
44	159058.0
45	176691.5
46	194325.0
47	208742.5
48	223160.0
49	229831.0
50	236502.0
51	223746.5
52	210991.0
53	208769.5
54	206548.0
55	210716.0
56	214884.0
57	209157.5
58	203431.0
59	186113.5
60	168796.0
61	148900.0
62	129004.0
63	113480.5
64	97957.0
65	83551.5
66	69146.0
67	59301.0
68	49456.0
69	45157.5
70	40859.0
71	31294.0
72	21729.0
73	18557.5
74	15386.0
75	11891.0
76	8396.0
77	7565.5
78	6735.0
79	5567.5
80	4400.0
81	3684.5
82	2969.0
83	2470.5
84	1972.0
85	1418.0
86	864.0
87	618.5
88	373.0
89	301.5
90	230.0
91	194.0
92	158.0
93	126.0
94	94.0
95	97.5
96	101.0
97	66.5
98	32.0
99	45.5
100	59.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007759332578531875
2	3.3018436504390956E-4
3	3.3018436504390956E-4
4	0.0
5	0.0
6	2.311290555307367E-4
7	3.3018436504390956E-5
8	3.3018436504390956E-5
9	0.0011556452776536835
10	7.92442476105383E-4
11	3.3018436504390956E-5
12	1.9811061902634574E-4
13	2.971659285395186E-4
14	1.3207374601756383E-4
15	0.004325415182075215
16	7.59424039600992E-4
17	0.0
18	3.962212380526915E-4
19	0.0
20	6.273502935834282E-4
21	0.0
22	6.603687300878191E-5
23	8.254609126097739E-4
24	3.632028015483005E-4
25	0.001551866515706375
26	0.005282949840702553
27	0.00653765042786941
28	0.004424470491588388
29	0.002377327428316149
30	0.0034999542694654413
31	0.007957443197558221
32	0.003268825213934705
33	0.00412730456304887
34	0.0025424196108381034
35	0.0029716592853951864
36	0.008023480070567003
37	0.003466935832961051
38	0.008452719745124085
39	0.004061267690040087
40	0.0024103458648205398
41	0.0033018436504390954
42	0.0018820508807502847
43	0.0011226268411492926
44	0.0013537558966800294
45	9.575346586273377E-4
46	0.0011226268411492926
47	0.002344308991811758
48	0.0020141246267678485
49	3.632028015483005E-4
50	0.0014858296426975932
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3028611.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.76433667526179
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.91990241462395	28.200828765918178
2	14.640098102532335	11.64307579815914
3	5.637566904198593	6.725223252235985
4	2.7260287416155236	4.335948986721596
5	1.5265929330524326	3.035197767798615
6	0.9318407953803605	2.2232418669149023
7	0.6246345001694762	1.7386723594606042
8	0.4272789629991783	1.359237163116484
9	0.3370026098998282	1.206061671644881
>10	1.9487658849392564	14.361713936122097
>50	0.14705247770048438	4.069088900416916
>100	0.10463395631835823	8.478161341958124
>500	0.017344481185402157	4.7107938705677945
>1k	0.011257235384736436	7.91275431896464
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	4161	0.13738971429477076	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	4083	0.13481427624742828	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	4046	0.13359259409676583	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	3757	0.12405026594699683	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	3677	0.12140879102664555	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	3671	0.1212106804076192	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	3626	0.1197248507649216	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	3601	0.11889938985231183	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	3582	0.1182720395587284	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	3317	0.10952215388506481	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	3226	0.10651747616316522	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	3209	0.10595616274259058	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.603687300878191E-5
2	0.0	0.0	0.0	0.0	6.603687300878191E-5
3	0.0	0.0	0.0	3.3018436504390956E-5	6.603687300878191E-5
4	0.0	0.0	0.0	3.3018436504390956E-5	6.603687300878191E-5
5	0.0	0.0	0.0	6.603687300878191E-5	6.603687300878191E-5
6	0.0	0.0	0.0	6.603687300878191E-5	6.603687300878191E-5
7	0.0	0.0	0.0	6.603687300878191E-5	6.603687300878191E-5
8	0.0	0.0	0.0	6.603687300878191E-5	6.603687300878191E-5
9	0.0	0.0	0.0	6.603687300878191E-5	6.603687300878191E-5
10	0.0	0.0	0.0	6.603687300878191E-5	6.603687300878191E-5
11	0.0	0.0	0.0	6.603687300878191E-5	6.603687300878191E-5
12	0.0	0.0	0.0	6.603687300878191E-5	3.632028015483005E-4
13	0.0	0.0	0.0	6.603687300878191E-5	4.622581110614734E-4
14	0.0	0.0	0.0	6.603687300878191E-5	4.622581110614734E-4
15	0.0	0.0	0.0	1.6509218252195478E-4	5.613134205746463E-4
16	0.0	0.0	0.0	1.6509218252195478E-4	5.613134205746463E-4
17	0.0	0.0	0.0	1.6509218252195478E-4	5.613134205746463E-4
18	0.0	0.0	0.0	1.9811061902634574E-4	5.943318570790372E-4
19	0.0	0.0	0.0	1.9811061902634574E-4	5.943318570790372E-4
20	0.0	0.0	0.0	2.311290555307367E-4	6.273502935834282E-4
21	0.0	0.0	0.0	3.3018436504390956E-4	6.603687300878191E-4
22	0.0	0.0	0.0	4.622581110614734E-4	7.59424039600992E-4
23	0.0	0.0	0.0	9.245162221229468E-4	7.59424039600992E-4
24	0.0	0.0	0.0	0.002113179936281021	8.25460912609774E-4
25	0.0	0.0	0.0	0.002542419610838104	8.25460912609774E-4
26	0.0	0.0	0.0	0.003037696158403968	8.25460912609774E-4
27	0.0	0.0	0.0	0.003830138634509351	9.245162221229468E-4
28	0.0	0.0	0.0	0.006570668864373801	9.245162221229468E-4
29	0.0	0.0	0.0	0.012282858379633436	9.245162221229468E-4
30	0.0	0.0	0.0	0.022155370894446334	9.245162221229468E-4
31	0.0	0.0	0.0	0.046390903288669294	9.575346586273378E-4
32	0.0	0.0	0.0	0.0722773575081118	9.575346586273378E-4
33	0.0	0.0	0.0	0.09964964137025191	9.575346586273378E-4
34	0.0	0.0	0.0	0.12863982862110718	9.905530951317288E-4
35	0.0	0.0	0.0	0.16562047750602504	9.905530951317288E-4
36	0.0	0.0	0.0	0.21838393904004177	9.905530951317288E-4
37	0.0	0.0	0.0	0.2953499145317771	9.905530951317288E-4
38	0.0	0.0	0.0	0.39450427935446314	9.905530951317288E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATATAC	945	0.0	20.25361	1
GTATCAA	8380	0.0	19.846907	1
GTCCTAC	825	0.0	17.066366	1
ATACACT	1125	0.0	17.012472	4
CGTCGTA	1010	0.0	16.771704	10
AACGCAG	9815	0.0	16.765326	6
TCCTACA	905	0.0	16.529549	2
CGGTTAT	80	0.0019881998	16.501343	32
CGAGCCG	1505	0.0	16.371805	15
CGCCCCT	840	0.0	16.237272	5
CCTACAG	1115	0.0	16.178553	3
ATACCGT	1070	0.0	15.625376	6
CGCAATA	1305	0.0	15.510585	36
GTATAAA	440	0.0	15.499726	1
GCTAGGA	1625	0.0	15.434848	35
TGTAGGA	1070	0.0	15.419779	2
TCTAGAT	930	0.0	15.3755665	2
ATACGAA	1310	0.0	15.281416	40
CTAGCGG	1360	0.0	15.20712	29
GTAGGAC	1085	0.0	15.206603	3
>>END_MODULE