FastQCFastQC Report
Thu 2 Feb 2017
SRR4064164_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064164_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3743563
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT77390.20672818916096777No Hit
TATCAACGCAGAGTACTTTTTTTTT46460.1241063660475328No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG250.00603836318.997425
TTTTACG453.552482E-516.8825342
GGTATCA14900.013.8991131
GTATCAA45450.013.1472111
CGAACGA3100.012.56684116
ACCGTAT1301.9826984E-1012.4230518
CGTTATT3650.011.9680982
CGACCAT5350.011.54233910
CGTCCAA1501.7644197E-1011.40028110
GGTCTAA1251.8351784E-811.3998231
AATTGCA5550.011.29848214
AACCGCG2300.011.1520017
CGATAAC3600.011.08360710
TATGCCG1201.2794953E-711.0818295
TACCGGC2150.011.0463417
GATAACG3500.010.857409511
TATACCG1053.475514E-610.8556685
GCGTTAT3950.010.8226181
CGTCGTA5100.010.80418810
TTATACT3450.010.7373854
Produced by FastQC (version 0.11.5)