##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064164_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3743563
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.287640678145394	32.0	32.0	32.0	32.0	32.0
2	30.872064661393438	32.0	32.0	32.0	32.0	32.0
3	30.891952666483775	32.0	32.0	32.0	32.0	32.0
4	30.927359309833975	32.0	32.0	32.0	32.0	32.0
5	30.816396571928934	32.0	32.0	32.0	32.0	32.0
6	34.48431534343084	36.0	36.0	36.0	32.0	36.0
7	34.43900743756683	36.0	36.0	36.0	32.0	36.0
8	34.40393096095885	36.0	36.0	36.0	32.0	36.0
9	34.55825452917448	36.0	36.0	36.0	32.0	36.0
10	34.23818538648875	36.0	36.0	36.0	32.0	36.0
11	34.52468811129931	36.0	36.0	36.0	32.0	36.0
12	34.33222200347637	36.0	36.0	36.0	32.0	36.0
13	34.41885497853249	36.0	36.0	36.0	32.0	36.0
14	34.3142383339081	36.0	36.0	36.0	32.0	36.0
15	34.2474946995683	36.0	36.0	36.0	32.0	36.0
16	34.24995839525073	36.0	36.0	36.0	32.0	36.0
17	34.16810722832766	36.0	36.0	36.0	32.0	36.0
18	34.16329924192541	36.0	36.0	36.0	32.0	36.0
19	34.190764253199426	36.0	36.0	36.0	32.0	36.0
20	34.167864411524526	36.0	36.0	36.0	32.0	36.0
21	34.16244657829987	36.0	36.0	36.0	32.0	36.0
22	34.13856585290537	36.0	36.0	36.0	32.0	36.0
23	34.08074393298577	36.0	36.0	36.0	32.0	36.0
24	34.06091469543854	36.0	36.0	36.0	32.0	36.0
25	33.6575532453975	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	9.0
4	38.0
5	61.0
6	142.0
7	53.0
8	220.0
9	234.0
10	350.0
11	94.0
12	233.0
13	188.0
14	290.0
15	549.0
16	984.0
17	1298.0
18	1744.0
19	2331.0
20	3521.0
21	5098.0
22	8182.0
23	12588.0
24	19199.0
25	28239.0
26	39923.0
27	53130.0
28	72331.0
29	97990.0
30	129094.0
31	177797.0
32	251781.0
33	360915.0
34	781570.0
35	1693386.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.69285877299361	16.8400846815809	11.376187928545507	26.09086861687998
2	17.262325032450992	19.121067646327326	36.59838801582652	27.018219305395156
3	18.85838997618529	22.8691713737333	28.35280493978205	29.919633710299358
4	12.853071311355635	15.07591625903824	35.15861645599817	36.91239597360796
5	14.976948984283315	35.963591764200814	33.43501943113667	15.624439820379198
6	35.12702994778273	34.961361362431084	16.304423038646654	13.607185651139522
7	31.08032718468236	30.00381006530548	20.304602163454476	18.611260586557684
8	28.71749526484785	32.22989858437122	18.900685795398672	20.151920355382256
9	27.256757504351597	14.01421642240793	18.171946712335394	40.55707936090508
10	15.892016746529137	26.460296485409863	31.189581956855022	26.458104811205978
11	37.37605695199593	20.962000375781397	22.086030420118792	19.57591225210388
12	24.894914351788742	23.239257421481792	28.352383197629443	23.513445029100026
13	29.467604484805005	19.15458977227955	25.00084198348857	26.376963759426875
14	23.848285496088938	19.146106724497088	24.79411545359021	32.21149232582377
15	25.514617227418917	26.754670010180753	21.81546720949769	25.91524555290264
16	26.208284116670157	25.588842458831078	22.981809414369152	25.221064010129613
17	24.444078555227026	25.76488814214226	24.591221260396882	25.199812042233834
18	25.197913199693637	24.76710629555519	25.44562382791249	24.58935667683868
19	25.8291575498922	24.531766473348725	24.976215076126458	24.662860900632616
20	26.149648988670325	24.100256107276333	24.403414407237303	25.346680496816038
21	26.97028792469929	23.96390014261903	24.126087520233323	24.939724412448356
22	26.26631735126248	23.766110168018926	24.618154598799673	25.349417881918917
23	24.911161039397125	23.774487525117124	25.175405483152414	26.138945952333337
24	25.20233092929739	24.45807604766439	24.83996114048506	25.49963188255316
25	25.28152441161445	24.138477689643178	24.876537747649653	25.70346015109272
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	182.0
1	182.0
2	444.0
3	706.0
4	706.0
5	706.0
6	1939.5
7	3173.0
8	3173.0
9	3173.0
10	4085.5
11	4998.0
12	4998.0
13	4998.0
14	6798.5
15	8599.0
16	8599.0
17	8599.0
18	15036.0
19	21473.0
20	21473.0
21	21473.0
22	33685.5
23	45898.0
24	45898.0
25	45898.0
26	67516.5
27	89135.0
28	89135.0
29	89135.0
30	113898.0
31	138661.0
32	138661.0
33	138661.0
34	175186.0
35	211711.0
36	211711.0
37	211711.0
38	250138.5
39	288566.0
40	288566.0
41	288566.0
42	333675.0
43	378784.0
44	378784.0
45	378784.0
46	428871.5
47	478959.0
48	478959.0
49	478959.0
50	497715.5
51	516472.0
52	516472.0
53	516472.0
54	493357.5
55	470243.0
56	470243.0
57	470243.0
58	435978.5
59	401714.0
60	401714.0
61	401714.0
62	350664.5
63	299615.0
64	299615.0
65	299615.0
66	245865.5
67	192116.0
68	192116.0
69	192116.0
70	144303.0
71	96490.0
72	96490.0
73	96490.0
74	72567.0
75	48644.0
76	48644.0
77	48644.0
78	38106.5
79	27569.0
80	27569.0
81	27569.0
82	18686.5
83	9804.0
84	9804.0
85	9804.0
86	7424.0
87	5044.0
88	5044.0
89	5044.0
90	3576.5
91	2109.0
92	2109.0
93	2109.0
94	1506.5
95	904.0
96	904.0
97	904.0
98	1449.0
99	1994.0
100	1994.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.041270842777322035
2	0.006037029428915715
3	0.0020835765285638305
4	0.004327428174709495
5	0.009189106741358434
6	0.01463846073914076
7	0.0225720790594415
8	0.03576806374034576
9	0.04899076094084699
10	0.05692437926114773
11	0.05411956470346566
12	0.062213458141348224
13	0.06402990947394233
14	0.0670484241884002
15	0.058500417917369096
16	0.06549909805177581
17	0.061358657514245116
18	0.07768000698799513
19	0.07340600385247958
20	0.07861494517388914
21	0.07554300542023734
22	0.07781356958597999
23	0.08246154799585315
24	0.0777067195075921
25	0.07869508273268007
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3743563.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.898383696686594
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.78469244072378	22.259826707715234
2	16.1285008033528	11.25717218975448
3	7.059991532479065	7.391468801874384
4	3.774916436107996	5.269539288409022
5	2.21363154355509	3.8626081485037074
6	1.4765532152682297	3.0917592393004174
7	0.9920247112038186	2.4234041305729903
8	0.7298916353528506	2.037763067803667
9	0.5291446668584845	1.6619664253574518
>10	2.9682283577751116	18.789350926506355
>50	0.20527759473301244	4.972759368206652
>100	0.11393982048947945	7.941098714318086
>500	0.014032285925491902	3.4138564217800815
>1k	0.009097855702708803	5.419194700981449
>5k	7.710047205685426E-5	0.2082318689159977
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	7739	0.20672818916096777	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4646	0.1241063660475328	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8698763717880533E-4
2	0.0	2.671251959697219E-5	0.0	0.0	1.8698763717880533E-4
3	0.0	2.671251959697219E-5	0.0	0.0	1.8698763717880533E-4
4	0.0	2.671251959697219E-5	0.0	0.0	1.8698763717880533E-4
5	0.0	2.671251959697219E-5	0.0	2.671251959697219E-5	2.1370015677577752E-4
6	0.0	2.671251959697219E-5	0.0	2.671251959697219E-5	3.7397527435761066E-4
7	0.0	2.671251959697219E-5	0.0	2.671251959697219E-5	3.7397527435761066E-4
8	2.671251959697219E-5	2.671251959697219E-5	0.0	2.671251959697219E-5	3.7397527435761066E-4
9	2.671251959697219E-5	2.671251959697219E-5	0.0	2.671251959697219E-5	3.7397527435761066E-4
10	2.671251959697219E-5	2.671251959697219E-5	0.0	2.671251959697219E-5	4.2740031355155503E-4
11	2.671251959697219E-5	2.671251959697219E-5	0.0	2.671251959697219E-5	4.2740031355155503E-4
12	2.671251959697219E-5	2.671251959697219E-5	0.0	2.671251959697219E-5	0.0012020633818637485
13	2.671251959697219E-5	2.671251959697219E-5	0.0	2.671251959697219E-5	0.0014959010974304426
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATGCG	25	0.006038363	18.99742	5
TTTTACG	45	3.552482E-5	16.882534	2
GGTATCA	1490	0.0	13.899113	1
GTATCAA	4545	0.0	13.147211	1
CGAACGA	310	0.0	12.566841	16
ACCGTAT	130	1.9826984E-10	12.423051	8
CGTTATT	365	0.0	11.968098	2
CGACCAT	535	0.0	11.542339	10
CGTCCAA	150	1.7644197E-10	11.400281	10
GGTCTAA	125	1.8351784E-8	11.399823	1
AATTGCA	555	0.0	11.298482	14
AACCGCG	230	0.0	11.152001	7
CGATAAC	360	0.0	11.083607	10
TATGCCG	120	1.2794953E-7	11.081829	5
TACCGGC	215	0.0	11.046341	7
GATAACG	350	0.0	10.8574095	11
TATACCG	105	3.475514E-6	10.855668	5
GCGTTAT	395	0.0	10.822618	1
CGTCGTA	510	0.0	10.804188	10
TTATACT	345	0.0	10.737385	4
>>END_MODULE