FastQCFastQC Report
Thu 2 Feb 2017
SRR4064164_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064164_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3743563
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48750.029.4223481
GGTATCA17750.027.0185781
TCAACGC74400.019.0993044
CAACGCA76650.018.5673565
ATCAACG76900.018.5355993
TATCAAC78000.018.4716052
AACGCAG78550.018.2022536
ACCGTCG7400.017.8354178
CGTCGTA7600.017.07754110
ATACCGT8150.017.0036016
TACCGTC7900.016.9850377
CCGTCGT8200.015.5596949
GTCGTAG8300.015.3722311
TACGACG9750.015.3413035
ACGGTAT9350.015.292869
ACGCAGA93150.015.2786567
CGCAGAG95400.014.9413688
AGAGTAC95950.014.92529611
ACGACGG9600.014.8936136
GTATTAT3550.014.8726121
Produced by FastQC (version 0.11.5)