FastQCFastQC Report
Thu 2 Feb 2017
SRR4064163_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064163_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14732
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT1551.0521314146076568No Hit
TATCAACGCAGAGTACTTTTTTTTT1170.7941895194135216No Hit
GTACTTTTTTTTTTTTTTTTTTTTT470.31903339668748304No Hit
GGTATCAACGCAGAGTACTTTTTTT390.2647298398045072No Hit
ACGCAGAGTACTTTTTTTTTTTTTT360.24436600597339125No Hit
GAGTACTTTTTTTTTTTTTTTTTTT300.20363833831115938No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG260.17648655986967146No Hit
CTTTAATATACGCTATTGGAGCTGG160.10860711376595167No Hit
ATCAACGCAGAGTACTTTTTTTTTT150.10181916915557969No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA350.002048101316.2711351
Produced by FastQC (version 0.11.5)