##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064163_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14732
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.949769209883247	32.0	32.0	32.0	21.0	32.0
2	28.750067879446103	32.0	32.0	32.0	14.0	32.0
3	28.837496606027695	32.0	32.0	32.0	14.0	32.0
4	28.71497420581048	32.0	32.0	32.0	14.0	32.0
5	28.768259571001902	32.0	32.0	32.0	14.0	32.0
6	32.047515612272605	36.0	32.0	36.0	21.0	36.0
7	31.98710290524029	36.0	32.0	36.0	21.0	36.0
8	31.86437686668477	36.0	32.0	36.0	14.0	36.0
9	32.15490089600869	36.0	32.0	36.0	14.0	36.0
10	31.288351887048602	36.0	32.0	36.0	14.0	36.0
11	32.330776540863425	36.0	32.0	36.0	21.0	36.0
12	31.87340483301656	36.0	32.0	36.0	14.0	36.0
13	32.10419494976921	36.0	32.0	36.0	14.0	36.0
14	31.82643225631279	36.0	32.0	36.0	14.0	36.0
15	31.63155036654901	36.0	32.0	36.0	14.0	36.0
16	31.725767037740972	36.0	32.0	36.0	14.0	36.0
17	31.475495519956556	36.0	32.0	36.0	14.0	36.0
18	31.555389628020635	36.0	32.0	36.0	14.0	36.0
19	31.460154765137116	36.0	32.0	36.0	14.0	36.0
20	31.658362747759977	36.0	32.0	36.0	14.0	36.0
21	31.52823784957915	36.0	32.0	36.0	14.0	36.0
22	31.510996470268804	36.0	32.0	36.0	14.0	36.0
23	31.37096117295683	36.0	32.0	36.0	14.0	36.0
24	31.25251153950584	36.0	32.0	36.0	14.0	36.0
25	30.456489275047517	36.0	27.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	10.0
15	21.0
16	47.0
17	56.0
18	101.0
19	112.0
20	151.0
21	195.0
22	221.0
23	288.0
24	315.0
25	413.0
26	464.0
27	609.0
28	713.0
29	776.0
30	900.0
31	1247.0
32	1488.0
33	1685.0
34	2457.0
35	2462.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.03190767141887	18.995247793618468	12.885268160217244	27.08757637474542
2	15.477564320141198	19.713529291969316	39.26413685425293	25.544769533636547
3	17.411077925604125	24.82351344013033	28.753733369535706	29.011675264729842
4	12.48303013847407	15.211783871843606	36.73635623133315	35.568829758349175
5	14.797719250610916	35.3448275862069	34.340211783871844	15.517241379310345
6	32.33098017920174	36.75672006516427	17.064892750475156	13.847407005158837
7	28.353244637523755	31.265272875373334	21.456692913385826	18.924789573717078
8	26.861720181929265	32.272079288575114	20.33127418369425	20.53492634580137
9	26.322221467852536	13.782334170683686	19.125534659515242	40.769909701948535
10	14.721260270251918	27.065933319752833	32.15862022136212	26.05418618863312
11	35.24579033134167	22.189027702335686	23.37724063009234	19.18794133623031
12	24.509471111412857	24.007060900264783	29.092266956344627	22.39120103197773
13	28.678117998506348	20.34082422431937	25.14766786611447	25.83338991105981
14	23.188701025327628	19.148502750050927	27.11346506416786	30.54933116045359
15	23.38403041825095	27.34926670287887	24.09695817490494	25.169744703965236
16	23.77104834329169	26.49375339489408	25.09505703422053	24.6401412275937
17	22.635616810374092	25.527870188064362	26.77710638875687	25.05940661280467
18	22.801656820805324	25.592449242887216	28.07768045087255	23.528213485434915
19	22.918647290506584	24.84720901806329	28.324052695911995	23.910090995518132
20	24.69781339128073	24.18851011815836	27.597446692924084	23.516229797636832
21	25.137539903552263	24.376825375263195	26.80839502818719	23.67723969299735
22	24.309092143681674	24.315882392883818	27.941875466829636	23.433149996604875
23	23.560564910374797	23.940793047256925	27.817762085822924	24.680879956545358
24	23.157894736842106	25.263157894736842	27.470288624787777	24.108658743633278
25	23.427950563628954	24.127393725383676	27.896237946489205	24.54841776449817
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	4.0
2	7.5
3	11.0
4	11.0
5	11.0
6	31.5
7	52.0
8	52.0
9	52.0
10	56.0
11	60.0
12	60.0
13	60.0
14	59.5
15	59.0
16	59.0
17	59.0
18	104.5
19	150.0
20	150.0
21	150.0
22	258.0
23	366.0
24	366.0
25	366.0
26	494.5
27	623.0
28	623.0
29	623.0
30	738.0
31	853.0
32	853.0
33	853.0
34	968.0
35	1083.0
36	1083.0
37	1083.0
38	1162.0
39	1241.0
40	1241.0
41	1241.0
42	1364.0
43	1487.0
44	1487.0
45	1487.0
46	1608.5
47	1730.0
48	1730.0
49	1730.0
50	1755.0
51	1780.0
52	1780.0
53	1780.0
54	1686.5
55	1593.0
56	1593.0
57	1593.0
58	1462.0
59	1331.0
60	1331.0
61	1331.0
62	1126.0
63	921.0
64	921.0
65	921.0
66	772.5
67	624.0
68	624.0
69	624.0
70	476.0
71	328.0
72	328.0
73	328.0
74	260.0
75	192.0
76	192.0
77	192.0
78	151.5
79	111.0
80	111.0
81	111.0
82	85.5
83	60.0
84	60.0
85	60.0
86	41.5
87	23.0
88	23.0
89	23.0
90	19.0
91	15.0
92	15.0
93	15.0
94	10.5
95	6.0
96	6.0
97	6.0
98	17.5
99	29.0
100	29.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013575889220743959
2	0.006787944610371979
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.006787944610371979
9	0.020363833831115938
10	0.033939723051859896
11	0.027151778441487917
12	0.020363833831115938
13	0.020363833831115938
14	0.033939723051859896
15	0.027151778441487917
16	0.027151778441487917
17	0.020363833831115938
18	0.033939723051859896
19	0.040727667662231876
20	0.040727667662231876
21	0.06109150149334781
22	0.033939723051859896
23	0.027151778441487917
24	0.047515612272603855
25	0.040727667662231876
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	14732.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.062177572631
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.48775923904569	82.26310073309801
2	3.2356151567129268	5.633994026608743
3	1.0369561827537814	2.7083898995384197
4	0.4755964447216592	1.6562584849307629
5	0.2261032278184937	0.984251968503937
6	0.12474660845158272	0.65164268259571
7	0.08576329331046312	0.5226717349986424
8	0.07016996725401528	0.4887320119467825
9	0.04677997816934352	0.3665490089600869
>10	0.194916575705598	2.878088514797719
>50	0.0	0.0
>100	0.01559332605644784	1.8463209340211784
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	155	1.0521314146076568	No Hit
TATCAACGCAGAGTACTTTTTTTTT	117	0.7941895194135216	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	47	0.31903339668748304	No Hit
GGTATCAACGCAGAGTACTTTTTTT	39	0.2647298398045072	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	36	0.24436600597339125	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	30	0.20363833831115938	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG	26	0.17648655986967146	No Hit
CTTTAATATACGCTATTGGAGCTGG	16	0.10860711376595167	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	15	0.10181916915557969	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	35	0.0020481013	16.271135	1
>>END_MODULE