##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064163_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14732
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.611797447732826	32.0	32.0	32.0	21.0	32.0
2	29.49545207711105	32.0	32.0	32.0	14.0	32.0
3	30.5200923160467	32.0	32.0	32.0	32.0	32.0
4	30.962598425196852	32.0	32.0	32.0	32.0	32.0
5	30.56604670105892	32.0	32.0	32.0	32.0	32.0
6	34.21857181645398	36.0	36.0	36.0	32.0	36.0
7	34.26418680423568	36.0	36.0	36.0	32.0	36.0
8	34.14519413521586	36.0	36.0	36.0	32.0	36.0
9	34.46619603584035	36.0	36.0	36.0	32.0	36.0
10	34.02660874287266	36.0	36.0	36.0	32.0	36.0
11	34.624423024708115	36.0	36.0	36.0	32.0	36.0
12	34.37503393972305	36.0	36.0	36.0	32.0	36.0
13	34.536519142003804	36.0	36.0	36.0	32.0	36.0
14	34.36200108607114	36.0	36.0	36.0	32.0	36.0
15	34.23377681238121	36.0	36.0	36.0	32.0	36.0
16	34.30871572087972	36.0	36.0	36.0	32.0	36.0
17	34.32086614173228	36.0	36.0	36.0	32.0	36.0
18	34.28190333966875	36.0	36.0	36.0	32.0	36.0
19	34.32025522671735	36.0	36.0	36.0	32.0	36.0
20	34.31217757263101	36.0	36.0	36.0	32.0	36.0
21	34.267988053217486	36.0	36.0	36.0	32.0	36.0
22	34.33376323649199	36.0	36.0	36.0	32.0	36.0
23	34.20954385012218	36.0	36.0	36.0	32.0	36.0
24	34.07772196578876	36.0	36.0	36.0	32.0	36.0
25	34.18300298669563	36.0	36.0	36.0	32.0	36.0
26	34.00746673907141	36.0	36.0	36.0	32.0	36.0
27	33.97407005158838	36.0	36.0	36.0	32.0	36.0
28	33.951601954928044	36.0	36.0	36.0	32.0	36.0
29	33.92370350257942	36.0	36.0	36.0	32.0	36.0
30	33.86892478957372	36.0	36.0	36.0	32.0	36.0
31	33.899199022535974	36.0	36.0	36.0	32.0	36.0
32	33.81740428998099	36.0	36.0	36.0	32.0	36.0
33	33.83525658430627	36.0	36.0	36.0	32.0	36.0
34	33.67132772196579	36.0	36.0	36.0	27.0	36.0
35	33.721286994298126	36.0	36.0	36.0	32.0	36.0
36	33.61478414336139	36.0	36.0	36.0	27.0	36.0
37	33.68212055389628	36.0	36.0	36.0	32.0	36.0
38	33.628156394243824	36.0	36.0	36.0	27.0	36.0
39	33.54961987510182	36.0	36.0	36.0	21.0	36.0
40	33.51242193863698	36.0	36.0	36.0	21.0	36.0
41	33.54697257670377	36.0	36.0	36.0	27.0	36.0
42	33.44956557154494	36.0	36.0	36.0	21.0	36.0
43	33.423431984795	36.0	36.0	36.0	21.0	36.0
44	33.41148520228075	36.0	36.0	36.0	21.0	36.0
45	33.30511811023622	36.0	36.0	36.0	21.0	36.0
46	33.17533260928591	36.0	36.0	36.0	21.0	36.0
47	33.28339668748303	36.0	36.0	36.0	21.0	36.0
48	33.107792560412705	36.0	36.0	36.0	21.0	36.0
49	33.22176215042085	36.0	36.0	36.0	21.0	36.0
50	32.3193049144719	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	1.0
22	2.0
23	10.0
24	26.0
25	43.0
26	110.0
27	173.0
28	320.0
29	475.0
30	702.0
31	1096.0
32	1509.0
33	2304.0
34	3472.0
35	4487.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.02022944810264	18.607019211187293	13.230602131559296	26.142149209150773
2	18.429269617159925	20.723594895465652	35.44664675536248	25.400488732011944
3	21.273418408905783	23.07901167526473	29.466467553624764	26.181102362204722
4	15.829486831387458	16.956285636709204	35.69780070594624	31.5164268259571
5	20.16698343741515	32.758620689655174	30.620418137388	16.453977735541677
6	34.22481672549552	36.36980722237306	15.91094216671192	13.494433885419497
7	29.283193049144717	30.518598968232418	19.705403203909853	20.492804778713005
8	27.728753733369537	32.77219657887592	18.096660331251694	21.40238935650285
9	26.31864774964361	13.413889077455707	17.15430045482316	43.113162718077525
10	14.702688026065708	26.004615802335053	31.319576432256312	27.973119739342927
11	38.50122183002987	20.26880260657073	21.544936193320662	19.68503937007874
12	24.86424110779256	22.888949226174315	28.482215585120823	23.7645940809123
13	29.43931577518327	18.619332066250337	24.701330437143632	27.240021721422753
14	23.581319576432254	17.45859353787673	25.393700787401574	33.566386098289435
15	25.271517784414883	25.549823513440128	22.753190333966874	26.425468368178116
16	25.156122726038554	25.393700787401574	23.45234862883519	25.99782785772468
17	23.431984795004073	25.42085256584306	24.86424110779256	26.282921531360305
18	24.436600597339126	24.97284822155851	26.669834374151506	23.920716806950857
19	25.624490904154225	24.239750203638337	25.400488732011944	24.735270160195494
20	25.45479228889492	24.151506923703504	25.380124898180835	25.013575889220746
21	26.418680423567743	23.581319576432254	25.28509367363562	24.71490632636438
22	25.48194406733641	23.947868585392342	25.128970947597068	25.441216399674175
23	23.676350800977463	24.409448818897637	25.088243279934836	26.825957100190063
24	24.056475699158295	25.07466739071409	25.515883790388273	25.352973119739342
25	24.3212055389628	24.41623676350801	25.20363833831116	26.05891935921803
26	24.27533772316883	24.404317425836673	25.592288371461542	25.728056479532956
27	25.427640510453436	24.769209883247353	25.40727667662232	24.395872929676894
28	25.07466739071409	24.484116209611727	25.515883790388273	24.925332609285906
29	25.149334781428184	24.79636166168884	25.183274504480043	24.871029052402932
30	24.653814824871027	24.721694270974748	25.760249796361663	24.86424110779256
31	25.056004344579456	24.377163804222388	25.137465209422306	25.429366641775847
32	25.312245452077107	24.327993483573174	25.48194406733641	24.877816997013305
33	24.402660874287267	24.918544664675537	25.760249796361663	24.918544664675537
34	25.393700787401574	23.554167797990768	25.77382568558241	25.278305729025252
35	25.556611458050504	24.90496877545479	24.63345099103991	24.90496877545479
36	24.708118381754005	24.925332609285906	24.62666304642954	25.739885962530547
37	25.563399402660874	24.022535976106436	25.16291067064893	25.251153950583767
38	25.352973119739342	24.3212055389628	25.149334781428184	25.17648655986967
39	24.899871020297333	24.804833344647342	25.171407236440157	25.12388839861516
40	24.877816997013305	25.61091501493348	24.55878360032582	24.9524843877274
41	25.409001425565137	25.334328966125856	25.130676804018737	24.125992804290274
42	25.977464023893564	25.027151778441485	25.06787944610372	23.927504751561226
43	24.871029052402932	24.93212055389628	25.1221830029867	25.07466739071409
44	25.101819169155583	25.3393972305186	24.477328265001358	25.081455335324463
45	25.081455335324463	25.24436600597339	24.375509095845775	25.298669562856368
46	24.266901982079826	25.522671734998642	24.647026880260654	25.563399402660874
47	24.68775454792289	25.06787944610372	24.90496877545479	25.3393972305186
48	25.495519956557157	25.984251968503933	23.703502579418952	24.816725495519957
49	24.531631821884332	25.95031224545208	24.54520771110508	24.97284822155851
50	25.712734184089058	24.71490632636438	24.884604941623675	24.68775454792289
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.5
4	3.0
5	2.5
6	2.0
7	1.5
8	1.0
9	1.5
10	2.0
11	4.0
12	6.0
13	12.0
14	18.0
15	22.5
16	27.0
17	30.5
18	34.0
19	34.0
20	34.0
21	42.5
22	51.0
23	71.0
24	91.0
25	115.5
26	140.0
27	156.5
28	173.0
29	193.0
30	213.0
31	253.5
32	294.0
33	301.5
34	309.0
35	366.5
36	424.0
37	483.0
38	542.0
39	576.5
40	611.0
41	663.0
42	715.0
43	719.5
44	724.0
45	821.0
46	918.0
47	955.0
48	992.0
49	1024.0
50	1056.0
51	1025.5
52	995.0
53	984.0
54	973.0
55	1022.0
56	1071.0
57	1017.0
58	963.0
59	870.5
60	778.0
61	712.0
62	646.0
63	591.5
64	537.0
65	447.5
66	358.0
67	312.0
68	266.0
69	251.5
70	237.0
71	186.0
72	135.0
73	127.5
74	120.0
75	96.5
76	73.0
77	69.5
78	66.0
79	54.0
80	42.0
81	32.0
82	22.0
83	26.5
84	31.0
85	21.5
86	12.0
87	11.0
88	10.0
89	6.5
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	2.0
96	3.0
97	3.5
98	4.0
99	4.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006787944610371979
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.006787944610371979
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.006787944610371979
27	0.0
28	0.0
29	0.0
30	0.0
31	0.006787944610371979
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.006787944610371979
40	0.0
41	0.006787944610371979
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	14732.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.03774097203366
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.61177077955601	87.99891392886235
2	2.8689431373995133	5.2810209068694
3	0.744892691201416	2.0567472169427097
4	0.3097573567372225	1.1403746945424924
5	0.13275315288738107	0.6109150149334781
6	0.10325245224574084	0.5701873472712462
7	0.029500700641640235	0.19006244909041542
8	0.06637657644369054	0.4887320119467825
9	0.02212552548123018	0.18327450448004345
>10	0.1106276274061509	1.4797719250610915
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT	36	0.24436600597339125	No Hit
TATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATG	28	0.19006244909041542	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	20	0.13575889220743959	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.006787944610371979	0.0
34	0.0	0.0	0.0	0.006787944610371979	0.0
35	0.0	0.0	0.0	0.006787944610371979	0.0
36	0.0	0.0	0.0	0.006787944610371979	0.0
37	0.0	0.0	0.0	0.006787944610371979	0.0
38	0.0	0.0	0.0	0.006787944610371979	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE