FastQCFastQC Report
Thu 2 Feb 2017
SRR4064162_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064162_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1982859
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT70400.3550428951327351No Hit
TATCAACGCAGAGTACTTTTTTTTT49440.24933694226367079No Hit
GTACTTTTTTTTTTTTTTTTTTTTT47910.24162081116206446No Hit
GAGTACTTTTTTTTTTTTTTTTTTT30120.1519018750198577No Hit
GTCCTAAAGTGTGTATTTCTCATTT30110.15185144279043541No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26300.13263676338055302No Hit
GTCCTACAGTGGACATTTCTAAATT25710.12966126184463947No Hit
GTACATGGGGTGGTATCAACGCAAA24860.1253745223437471No Hit
GGTATCAACGCAGAGTACTTTTTTT24250.12229815634898901No Hit
CTGTAGGACGTGGAATATGGCAAGA23840.1202304349426762No Hit
CTTTAGGACGTGAAATATGGCGAGG23450.11826357799520792No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATT400.00527726814.25102211
GTTCGCC400.00527726814.25102211
GAACCGC1051.373337E-913.5700056
TACGAGA655.4657998E-513.1508074
AACCGCG957.396011E-812.9992917
CGTAAAC604.104386E-412.6624633
GCGTTAT1103.8293365E-812.08781051
TAGAAAT5550.011.8080814
AGAACCG1459.822543E-1111.7903795
TTTAGAA6350.011.6636512
TCGCGTA1157.072231E-811.5657559
GCGCCGA1406.7848305E-1011.5382919
GTCTATA1354.7493813E-911.2563741
CGAACGA1104.955891E-711.22977816
CGCGTAA1104.9672235E-711.22779610
CGAGCCG2550.011.17896515
CGCGCTA600.005866868411.08580816
CGAATTT600.005866868411.08580815
ATAGACG600.005891163411.0796563
ATGTCCA5750.011.0700799