##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064162_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1982859
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.219304045320417	32.0	32.0	32.0	32.0	32.0
2	30.7936313172041	32.0	32.0	32.0	32.0	32.0
3	30.821600022997096	32.0	32.0	32.0	32.0	32.0
4	30.83906520836832	32.0	32.0	32.0	32.0	32.0
5	30.75836305052452	32.0	32.0	32.0	32.0	32.0
6	34.41855321028878	36.0	36.0	36.0	32.0	36.0
7	34.34372338123891	36.0	36.0	36.0	32.0	36.0
8	34.292157939621525	36.0	36.0	36.0	32.0	36.0
9	34.44518243606832	36.0	36.0	36.0	32.0	36.0
10	34.11749952971946	36.0	36.0	36.0	32.0	36.0
11	34.44014324770445	36.0	36.0	36.0	32.0	36.0
12	34.23497586061339	36.0	36.0	36.0	32.0	36.0
13	34.31675878113371	36.0	36.0	36.0	32.0	36.0
14	34.22035101840323	36.0	36.0	36.0	32.0	36.0
15	34.16251937228013	36.0	36.0	36.0	32.0	36.0
16	34.15856195523736	36.0	36.0	36.0	32.0	36.0
17	34.08618767143806	36.0	36.0	36.0	32.0	36.0
18	34.0868478293212	36.0	36.0	36.0	32.0	36.0
19	34.09359364432872	36.0	36.0	36.0	32.0	36.0
20	34.07011996314413	36.0	36.0	36.0	32.0	36.0
21	34.05888971429638	36.0	36.0	36.0	32.0	36.0
22	34.0310854175713	36.0	36.0	36.0	32.0	36.0
23	33.990656925177234	36.0	36.0	36.0	32.0	36.0
24	33.96783331542989	36.0	36.0	36.0	32.0	36.0
25	33.587693325647464	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	5.0
4	15.0
5	28.0
6	80.0
7	35.0
8	117.0
9	139.0
10	189.0
11	65.0
12	119.0
13	91.0
14	212.0
15	351.0
16	613.0
17	790.0
18	1000.0
19	1318.0
20	2058.0
21	3160.0
22	5031.0
23	7462.0
24	11299.0
25	16416.0
26	23193.0
27	30365.0
28	41334.0
29	54843.0
30	72168.0
31	97355.0
32	136957.0
33	193801.0
34	413226.0
35	869023.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.699080263761985	17.884303941798805	11.718539521414264	25.698076273024938
2	15.424299751709864	20.48910584785559	39.657981975639025	24.428612424795524
3	18.44848559428371	25.151702823851235	29.470896055323117	26.928915526541942
4	12.046169333282226	16.291706425497836	37.50859900301999	34.15352523819995
5	13.442361669772756	37.70874050835617	34.59636398483859	14.25253383703248
6	31.912847072305638	37.007511409352645	18.01557966774674	13.064061850594982
7	28.519290875449204	31.54755779393338	21.886482363694697	18.04666896692272
8	26.772310595167824	34.69918723797127	20.231266364971017	18.297235801889887
9	27.471450657775232	14.289707704790317	19.383630903123976	38.855210734310475
10	15.27732151715027	27.50103573265028	33.28473842262837	23.936904327571078
11	35.5340123284851	21.430380794367814	24.509323063632586	18.526283813514507
12	24.551097043581475	24.037156714960435	30.59252010922746	20.81922613223063
13	29.274640292580372	20.81652612789743	25.929163211863898	23.9796703676583
14	22.3986042854533	20.931983644664076	26.808155866230226	29.8612562036524
15	23.679618333245863	29.543344246940894	23.297961671840874	23.47907574797237
16	23.520425697918597	26.571247323364787	26.468547296768758	23.439779681947858
17	22.26137764046747	27.123400630411766	27.015758275542222	23.59946345357854
18	22.660897373130894	26.010980702510583	28.956883733559984	22.37123819079854
19	24.71884196654922	25.411396925039835	26.754616402176485	23.11514470623446
20	24.838485967458958	25.61476278635933	26.666521977012515	22.88022926916919
21	25.157847559886175	24.97978642758292	25.822548555474018	24.039817457056884
22	24.729090642964938	26.163812465615838	26.15462653119937	22.952470360219856
23	23.07681048084659	26.127667177801083	26.944237220209427	23.851285121142894
24	23.940207146090938	25.83920614018928	27.207343435419144	23.01324327830064
25	23.523684640869256	26.03281508355188	27.186259011832732	23.25724126374614
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	709.0
1	709.0
2	1101.5
3	1494.0
4	1494.0
5	1494.0
6	3979.0
7	6464.0
8	6464.0
9	6464.0
10	6412.0
11	6360.0
12	6360.0
13	6360.0
14	6846.5
15	7333.0
16	7333.0
17	7333.0
18	12924.0
19	18515.0
20	18515.0
21	18515.0
22	29701.0
23	40887.0
24	40887.0
25	40887.0
26	60994.5
27	81102.0
28	81102.0
29	81102.0
30	103831.5
31	126561.0
32	126561.0
33	126561.0
34	143125.5
35	159690.0
36	159690.0
37	159690.0
38	173646.5
39	187603.0
40	187603.0
41	187603.0
42	204607.0
43	221611.0
44	221611.0
45	221611.0
46	238837.5
47	256064.0
48	256064.0
49	256064.0
50	254125.0
51	252186.0
52	252186.0
53	252186.0
54	225709.5
55	199233.0
56	199233.0
57	199233.0
58	178532.0
59	157831.0
60	157831.0
61	157831.0
62	136228.5
63	114626.0
64	114626.0
65	114626.0
66	93262.5
67	71899.0
68	71899.0
69	71899.0
70	53954.0
71	36009.0
72	36009.0
73	36009.0
74	27142.5
75	18276.0
76	18276.0
77	18276.0
78	14176.5
79	10077.0
80	10077.0
81	10077.0
82	7025.0
83	3973.0
84	3973.0
85	3973.0
86	3028.0
87	2083.0
88	2083.0
89	2083.0
90	1507.0
91	931.0
92	931.0
93	931.0
94	651.0
95	371.0
96	371.0
97	371.0
98	671.0
99	971.0
100	971.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.038782384425720635
2	0.004942358483381824
3	0.0017651280297792226
4	0.003580688288980709
5	0.00978385250791912
6	0.013868863091122464
7	0.023753580057886112
8	0.0367650952488301
9	0.051793899616664626
10	0.0590057084240483
11	0.055828477970445704
12	0.06586449162547614
13	0.0664696783785433
14	0.0688399931613897
15	0.062283803336495434
16	0.06858783201427837
17	0.06541060156067577
18	0.0789264390458424
19	0.07564834413339526
20	0.08104459268157746
21	0.07650569203357376
22	0.07912816796353145
23	0.08296101739962347
24	0.07827082006335297
25	0.07943076134006503
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1982859.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.01381767579593
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.93878694641418	37.71899796453892
2	14.987243478195907	15.291130125188952
3	4.94637978870467	7.570011500886658
4	2.153628381133116	4.394592223061773
5	1.1163340168947034	2.8474230001577636
6	0.6568511579311733	2.0105091126501784
7	0.40584048829435143	1.4492430872712823
8	0.3035848247039232	1.238961671726752
9	0.21860036277008826	1.0036475145194517
>10	1.1014765518586815	9.980069626866449
>50	0.08379115321756458	2.9798035186897014
>100	0.07704602316080163	7.937649607887123
>500	0.007653672928581643	2.642741844154341
>1k	0.0026837554424896668	2.5782426763857327
>5k	9.93983497218395E-5	0.3569765260149384
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	7040	0.3550428951327351	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4944	0.24933694226367079	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4791	0.24162081116206446	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	3012	0.1519018750198577	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3011	0.15185144279043541	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2630	0.13263676338055302	No Hit
GTCCTACAGTGGACATTTCTAAATT	2571	0.12966126184463947	No Hit
GTACATGGGGTGGTATCAACGCAAA	2486	0.1253745223437471	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2425	0.12229815634898901	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2384	0.1202304349426762	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2345	0.11826357799520792	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.521611471113175E-4
2	0.0	0.0	0.0	0.0	2.521611471113175E-4
3	0.0	0.0	0.0	0.0	2.521611471113175E-4
4	0.0	0.0	0.0	0.0	2.521611471113175E-4
5	0.0	0.0	0.0	0.0	2.521611471113175E-4
6	0.0	0.0	0.0	0.0	3.0259337653358104E-4
7	0.0	0.0	0.0	0.0	3.0259337653358104E-4
8	0.0	0.0	0.0	0.0	3.0259337653358104E-4
9	0.0	0.0	0.0	0.0	3.0259337653358104E-4
10	0.0	0.0	0.0	5.043222942226351E-5	3.5302560595584456E-4
11	0.0	0.0	0.0	5.043222942226351E-5	3.5302560595584456E-4
12	0.0	0.0	0.0	5.043222942226351E-5	5.04322294222635E-4
13	0.0	0.0	0.0	1.0086445884452702E-4	5.547545236448986E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCATT	40	0.005277268	14.251022	11
GTTCGCC	40	0.005277268	14.251022	11
GAACCGC	105	1.373337E-9	13.570005	6
TACGAGA	65	5.4657998E-5	13.150807	4
AACCGCG	95	7.396011E-8	12.999291	7
CGTAAAC	60	4.104386E-4	12.662463	3
GCGTTAT	110	3.8293365E-8	12.0878105	1
TAGAAAT	555	0.0	11.808081	4
AGAACCG	145	9.822543E-11	11.790379	5
TTTAGAA	635	0.0	11.663651	2
TCGCGTA	115	7.072231E-8	11.565755	9
GCGCCGA	140	6.7848305E-10	11.53829	19
GTCTATA	135	4.7493813E-9	11.256374	1
CGAACGA	110	4.955891E-7	11.229778	16
CGCGTAA	110	4.9672235E-7	11.227796	10
CGAGCCG	255	0.0	11.178965	15
CGCGCTA	60	0.0058668684	11.085808	16
CGAATTT	60	0.0058668684	11.085808	15
ATAGACG	60	0.0058911634	11.079656	3
ATGTCCA	575	0.0	11.070079	9
>>END_MODULE