FastQCFastQC Report
Thu 2 Feb 2017
SRR4064162_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064162_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1982859
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24540.12376069100223466No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG500.002581762421.9989035
TTATCGT708.128552E-418.8562034
GTATCAA52550.018.7987691
TAGGACG19500.018.1631974
TGTAGGA20950.017.9556922
CGTCGTA3200.017.8745610
TTAGGAC12600.017.8081873
ACCGTCG3000.017.5991238
GTATTAG4650.017.506711
GTAGGAC21500.017.2917253
GTCCTAC17200.017.2687591
TACCGTC3200.017.1866447
CTGTAGG21900.017.0789281
TCTATAC2350.016.84983
AGGACGT21050.016.8257645
GGACGTG20200.016.771446
CGTTAGG1053.777923E-516.7610715
ATACCGT3150.016.761076
GACGTGA11300.016.7425277
AACGCAG59200.016.722146
Produced by FastQC (version 0.11.5)