##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4064162_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1982859 Sequences flagged as poor quality 0 Sequence length 50 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.274951975909534 32.0 32.0 32.0 32.0 32.0 2 31.43725146366938 32.0 32.0 32.0 32.0 32.0 3 31.502728131450596 32.0 32.0 32.0 32.0 32.0 4 31.582416097160717 32.0 32.0 32.0 32.0 32.0 5 31.546496246077 32.0 32.0 32.0 32.0 32.0 6 35.13025131892888 36.0 36.0 36.0 36.0 36.0 7 35.147449213484165 36.0 36.0 36.0 36.0 36.0 8 35.101852930541206 36.0 36.0 36.0 36.0 36.0 9 35.195668981001674 36.0 36.0 36.0 36.0 36.0 10 35.06766391357126 36.0 36.0 36.0 36.0 36.0 11 35.19452063913773 36.0 36.0 36.0 36.0 36.0 12 35.1144756132433 36.0 36.0 36.0 36.0 36.0 13 35.14738365158592 36.0 36.0 36.0 36.0 36.0 14 35.10024968996787 36.0 36.0 36.0 36.0 36.0 15 35.075570678500085 36.0 36.0 36.0 36.0 36.0 16 35.0901733305293 36.0 36.0 36.0 36.0 36.0 17 35.06110772374637 36.0 36.0 36.0 36.0 36.0 18 35.04961119272727 36.0 36.0 36.0 36.0 36.0 19 35.04295968598877 36.0 36.0 36.0 36.0 36.0 20 35.034976264071226 36.0 36.0 36.0 36.0 36.0 21 35.02531697916998 36.0 36.0 36.0 36.0 36.0 22 35.012379095033985 36.0 36.0 36.0 36.0 36.0 23 34.97358914577386 36.0 36.0 36.0 36.0 36.0 24 34.95021885065958 36.0 36.0 36.0 36.0 36.0 25 34.921599064784736 36.0 36.0 36.0 36.0 36.0 26 34.86976028048389 36.0 36.0 36.0 32.0 36.0 27 34.856917208939215 36.0 36.0 36.0 32.0 36.0 28 34.82828027610637 36.0 36.0 36.0 32.0 36.0 29 34.803130731938076 36.0 36.0 36.0 32.0 36.0 30 34.776113682314275 36.0 36.0 36.0 32.0 36.0 31 34.75807861275058 36.0 36.0 36.0 32.0 36.0 32 34.72234485659343 36.0 36.0 36.0 32.0 36.0 33 34.69835827963562 36.0 36.0 36.0 32.0 36.0 34 34.673436184822016 36.0 36.0 36.0 32.0 36.0 35 34.628746169041776 36.0 36.0 36.0 32.0 36.0 36 34.59374922775649 36.0 36.0 36.0 32.0 36.0 37 34.564904514138426 36.0 36.0 36.0 32.0 36.0 38 34.52642321012235 36.0 36.0 36.0 32.0 36.0 39 34.49158109578139 36.0 36.0 36.0 32.0 36.0 40 34.48753441369255 36.0 36.0 36.0 32.0 36.0 41 34.45769568083258 36.0 36.0 36.0 32.0 36.0 42 34.40089486947887 36.0 36.0 36.0 32.0 36.0 43 34.37842932856043 36.0 36.0 36.0 32.0 36.0 44 34.30982334094356 36.0 36.0 36.0 32.0 36.0 45 34.26591300743018 36.0 36.0 36.0 32.0 36.0 46 34.209753694034724 36.0 36.0 36.0 32.0 36.0 47 34.17117001259293 36.0 36.0 36.0 32.0 36.0 48 34.11061300879185 36.0 36.0 36.0 32.0 36.0 49 34.076719524686325 36.0 36.0 36.0 32.0 36.0 50 33.50937812522222 36.0 36.0 36.0 21.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 0.0 17 2.0 18 1.0 19 5.0 20 19.0 21 75.0 22 223.0 23 661.0 24 1647.0 25 3865.0 26 7933.0 27 14457.0 28 24054.0 29 36526.0 30 50936.0 31 70408.0 32 100135.0 33 157118.0 34 320339.0 35 1194454.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.866841178814425 18.77528786938825 12.209343965460718 26.148526986336606 2 14.823900119625671 21.188772535721274 39.07089384381688 24.91643350083617 3 17.7382701903418 25.71021366172194 29.819795103421633 26.73172104451462 4 11.783238243364757 16.879818484319863 37.53378328968424 33.80315998263114 5 13.115506448012692 38.12893402909637 34.39155280330069 14.364006719590247 6 32.06874326728719 37.338919215515396 17.67707791186047 12.91525960533695 7 28.37356822684048 31.72323154076746 21.911878623561165 17.991321608830898 8 26.754852463034435 34.90112004938324 20.050391883638728 18.2936356039436 9 27.415204701956895 14.197708935795935 19.315434373225393 39.071651989021774 10 15.19812268986306 27.78544075988092 33.21816165423582 23.798274896020196 11 35.6435833309378 21.36435318900638 24.526050515946924 18.466012964108895 12 24.528571176409773 24.211150084095912 30.502230369845503 20.758048369648815 13 29.37169162739655 20.772898376476665 25.998210654573622 23.85719934155316 14 22.538735851825628 20.932161889963602 26.714284921776855 29.81481733643392 15 23.661363631778666 29.57628845389567 23.254961256105116 23.507386658220543 16 23.554856448905817 26.611621263959716 26.395215366987284 23.43830692014718 17 22.381520824224012 27.081249851855326 27.02960724892693 23.507622074993733 18 22.809933777172365 25.91798375167877 29.10037113227368 22.171711338875184 19 24.79598881815481 25.255426891601363 26.994483212859024 22.954101077384802 20 24.90832626017035 25.557090385692895 26.861023568636412 22.673559785500345 21 25.2599403184997 24.89940031035994 26.079010156546683 23.761649214593675 22 24.662418583305385 26.108616111616833 26.421599158788716 22.807366146289063 23 23.113517755305008 25.931187728333644 27.18010090556796 23.775193610793384 24 23.91329235185569 25.850708602327867 27.35586759845818 22.880131447358266 25 23.679221636582515 25.849153758749505 27.38670595064521 23.084918654022765 26 22.78432428724752 26.59602690423738 27.80607201362158 22.81357679489352 27 23.335391114621444 26.39235635123099 26.907053852338052 23.365198681809517 28 22.782528438821544 26.483471314048817 27.101244978603113 23.63275526852653 29 23.441856195859607 25.832758391130156 26.726793517072757 23.99859189593748 30 22.86820669373642 26.284623752823688 27.258660697939607 23.588508855500283 31 23.957030398889597 25.624046756348907 26.723252689256693 23.695670155504807 32 23.154560768214324 26.65081350427178 26.663573366687178 23.531052360826717 33 22.85399429584444 26.343716981084498 27.39210143410843 23.410187288962636 34 23.550160909708495 26.23915101405142 27.44094303529019 22.769745040949893 35 23.717001790904465 26.433467470447052 27.073321172061487 22.776209566586996 36 22.91190672892539 26.598809498591308 27.127786738649533 23.361497033833764 37 23.587055307362313 26.487789329494937 26.25014247715574 23.675012885987005 38 23.363275969899327 26.855468355962635 26.598946879980634 23.182308794157404 39 24.012739817224475 26.02120276785894 26.331729508362077 23.6343279065545 40 24.382704686244676 26.116966738501002 26.984223848763595 22.51610472649073 41 23.5991260834437 26.030488229259863 27.465984936438304 22.90440075085813 42 23.841205558763676 27.1349571190454 26.64691360515227 22.376923717038654 43 22.925355881667645 26.628461858906142 27.090328834358303 23.355853425067906 44 22.763070964227897 26.912745923755438 26.473575646928882 23.850607465087776 45 22.862791377936475 27.032055355503775 26.537103720963074 23.568049545596676 46 23.096401771804743 26.191613329789586 26.446853674810384 24.265131223595283 47 23.816142572771394 26.21025923193191 26.542009129426337 23.43158906587036 48 23.46534638485099 26.68915664595027 26.016928508999104 23.828568460199644 49 23.50389892548821 26.561259679230037 26.162692765074986 23.772148630206765 50 23.082757768548568 27.544097214342088 25.807341508496673 23.565803508612667 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 295.0 1 201.0 2 107.0 3 335.5 4 564.0 5 635.0 6 706.0 7 663.0 8 620.0 9 909.0 10 1198.0 11 1772.0 12 2346.0 13 3909.0 14 5472.0 15 7145.5 16 8819.0 17 9294.0 18 9769.0 19 9826.5 20 9884.0 21 10886.0 22 11888.0 23 13352.5 24 14817.0 25 16996.5 26 19176.0 27 24036.0 28 28896.0 29 34529.0 30 40162.0 31 47002.5 32 53843.0 33 63703.0 34 73563.0 35 84719.5 36 95876.0 37 102341.5 38 108807.0 39 109517.5 40 110228.0 41 111496.5 42 112765.0 43 112598.5 44 112432.0 45 117757.0 46 123082.0 47 128136.0 48 133190.0 49 133742.0 50 134294.0 51 130718.0 52 127142.0 53 123745.0 54 120348.0 55 118159.5 56 115971.0 57 110920.5 58 105870.0 59 96072.5 60 86275.0 61 75618.5 62 64962.0 63 56038.5 64 47115.0 65 39796.0 66 32477.0 67 27997.5 68 23518.0 69 20706.5 70 17895.0 71 14044.5 72 10194.0 73 8645.0 74 7096.0 75 5579.5 76 4063.0 77 3457.0 78 2851.0 79 2353.0 80 1855.0 81 1453.5 82 1052.0 83 895.0 84 738.0 85 516.5 86 295.0 87 213.5 88 132.0 89 102.5 90 73.0 91 61.0 92 49.0 93 39.0 94 29.0 95 29.0 96 29.0 97 21.5 98 14.0 99 15.5 100 17.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009531691360807804 2 3.5302560595584456E-4 3 2.521611471113175E-4 4 0.0 5 0.0 6 1.5129668826679052E-4 7 1.5129668826679052E-4 8 0.0 9 0.0012608057355565877 10 0.0011095090472897971 11 0.0 12 2.01728917689054E-4 13 5.547545236448986E-4 14 3.0259337653358104E-4 15 0.004791061795115033 16 5.547545236448986E-4 17 0.0 18 4.03457835378108E-4 19 1.00864458844527E-4 20 6.051867530671621E-4 21 0.0 22 2.01728917689054E-4 23 7.564834413339526E-4 24 6.051867530671621E-4 25 0.001613831341512432 26 0.006203164218938411 27 0.007110944348539154 28 0.00474062956569277 29 0.002572043700535439 30 0.00403457835378108 31 0.007816995560450844 32 0.003984146124358817 33 0.00474062956569277 34 0.0035302560595584453 35 0.003126798224180337 36 0.00832131785467348 37 0.004286739500892398 38 0.009027369066585168 39 0.004992790712804087 40 0.0028242048476467565 41 0.003076365994758074 42 0.0022190180945795943 43 0.0018659924886237497 44 0.0014625346532456418 45 0.001966856947468277 46 0.0011095090472897971 47 0.00201728917689054 48 0.0025216114711131754 49 2.521611471113175E-4 50 0.0012103735061343242 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1982859.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.27624489524479 #Duplication Level Percentage of deduplicated Percentage of total 1 75.69091149700394 42.59600271749693 2 14.319853726681941 16.117351903736733 3 4.601666379827826 7.768935123522157 4 1.9633539215849396 4.4196074442861315 5 0.9598900028253298 2.7009502435747734 6 0.5701004132106025 1.9249866281232062 7 0.39596352914572597 1.5598338375053191 8 0.25458356427001605 1.146160560733097 9 0.18806897152525362 0.9525433948876791 >10 0.9272664379439849 9.224757475866191 >50 0.06444253397428334 2.5170583669845312 >100 0.058206401010077284 6.4996828118413275 >500 0.004608312235081224 1.7798249244782989 >1k 0.0010843087611955798 0.7923045669636964 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2454 0.12376069100223466 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.0259337653358104E-4 2 0.0 0.0 0.0 0.0 3.0259337653358104E-4 3 0.0 0.0 0.0 0.0 3.0259337653358104E-4 4 0.0 0.0 0.0 0.0 3.0259337653358104E-4 5 0.0 0.0 0.0 0.0 3.0259337653358104E-4 6 0.0 0.0 0.0 0.0 3.0259337653358104E-4 7 0.0 0.0 0.0 5.043222942226351E-5 3.0259337653358104E-4 8 0.0 0.0 0.0 5.043222942226351E-5 3.0259337653358104E-4 9 0.0 0.0 0.0 5.043222942226351E-5 3.0259337653358104E-4 10 0.0 0.0 0.0 5.043222942226351E-5 3.0259337653358104E-4 11 0.0 0.0 0.0 5.043222942226351E-5 3.0259337653358104E-4 12 0.0 0.0 0.0 5.043222942226351E-5 6.051867530671621E-4 13 0.0 0.0 0.0 1.0086445884452702E-4 8.069156707562161E-4 14 0.0 0.0 0.0 1.0086445884452702E-4 8.069156707562161E-4 15 0.0 0.0 0.0 1.0086445884452702E-4 8.573479001784797E-4 16 0.0 0.0 0.0 1.0086445884452702E-4 8.573479001784797E-4 17 0.0 0.0 0.0 1.0086445884452702E-4 9.077801296007432E-4 18 0.0 0.0 0.0 1.0086445884452702E-4 9.582123590230067E-4 19 0.0 0.0 0.0 3.0259337653358104E-4 9.582123590230067E-4 20 0.0 0.0 0.0 3.5302560595584456E-4 0.0011095090472897971 21 0.0 0.0 0.0 5.04322294222635E-4 0.0012103735061343242 22 0.0 0.0 0.0 6.556189824894256E-4 0.0012608057355565877 23 0.0 0.0 0.0 0.0014121024238233782 0.0012608057355565877 24 0.0 0.0 0.0 0.00287463707706902 0.0012608057355565877 25 0.0 0.0 0.0 0.0032276626830248646 0.0012608057355565877 26 0.0 0.0 0.0 0.003933713894936554 0.0013112379649788512 27 0.0 0.0 0.0 0.004942358483381824 0.0013112379649788512 28 0.0 0.0 0.0 0.00892650460774064 0.0013112379649788512 29 0.0 0.0 0.0 0.01623917787396885 0.0013112379649788512 30 0.0 0.0 0.0 0.03030976988278037 0.0013112379649788512 31 0.0 0.0 0.0 0.059711759635959996 0.0013112379649788512 32 0.0 0.0 0.0 0.09208925092505317 0.0013112379649788512 33 0.0 0.0 0.0 0.12481976782010219 0.0013112379649788512 34 0.0 0.0 0.0 0.15785287809168477 0.0014121024238233782 35 0.0 0.0 0.0 0.19698828812336128 0.0014121024238233782 36 0.0 0.0 0.0 0.252816766093807 0.0014121024238233782 37 0.0 0.0 0.0 0.33996365853547833 0.0014121024238233782 38 0.0 0.0 0.0 0.45948804226624285 0.0015129668826679053 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACCG 50 0.0025817624 21.998903 5 TTATCGT 70 8.128552E-4 18.856203 4 GTATCAA 5255 0.0 18.798769 1 TAGGACG 1950 0.0 18.163197 4 TGTAGGA 2095 0.0 17.955692 2 CGTCGTA 320 0.0 17.87456 10 TTAGGAC 1260 0.0 17.808187 3 ACCGTCG 300 0.0 17.599123 8 GTATTAG 465 0.0 17.50671 1 GTAGGAC 2150 0.0 17.291725 3 GTCCTAC 1720 0.0 17.268759 1 TACCGTC 320 0.0 17.186644 7 CTGTAGG 2190 0.0 17.078928 1 TCTATAC 235 0.0 16.8498 3 AGGACGT 2105 0.0 16.825764 5 GGACGTG 2020 0.0 16.77144 6 CGTTAGG 105 3.777923E-5 16.76107 15 ATACCGT 315 0.0 16.76107 6 GACGTGA 1130 0.0 16.742527 7 AACGCAG 5920 0.0 16.72214 6 >>END_MODULE