FastQCFastQC Report
Thu 2 Feb 2017
SRR4064161_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064161_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences616088
Sequences flagged as poor quality0
Sequence length25
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT56240.9128566049005986No Hit
TATCAACGCAGAGTACTTTTTTTTT38800.6297801612756619No Hit
CCATAGGGTCTTCTCGTCTTATTAT33110.5374232252535353No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23850.3871200218150654No Hit
CCATTGGGATGTCCTGATCCAACAT22360.3629351651062835No Hit
GGTATCAACGCAGAGTACTTTTTTT21800.3538455545311709No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19850.3221942319928322No Hit
GTATTAGAGGCACTGCCTGCCCAGT19090.3098583319266079No Hit
GTATCAACGCAGAGTACATGGGAGA17670.2868096765397151No Hit
GAGTACTTTTTTTTTTTTTTTTTTT17340.28145329887938086No Hit
TCCCAATGGTGTAGAAGCTATTAAT17180.27885626728649154No Hit
CTCTAATACTTGTAATGCTAGAGGT16990.27577229226993544No Hit
CTTCTACACCATTGGGATGTCCTGA16420.26652036722026723No Hit
ATCGTAAATAGATAGAAACCGACCT15970.259216215865266No Hit
GATATATTTTGATCAACGGACCAAG15960.2590539013907104No Hit
GTGTAGAAGCTATTAATGGTTCGTT14550.23616756047837323No Hit
GTACATGGGAGAAATCGTAAATAGA14460.23470673020737298No Hit
GATTAAAGATAAGAGACAGTTGGAC14420.23405747230915067No Hit
GCCTAAAGGAAAGATCCAAAAAGAT14250.23129812624170573No Hit
GTTCATGCTAGTCCCTAATTAAGGA14080.22853878017426082No Hit
GTCAGGATACCGCGGCCGTTAAACT13930.2261040630559271No Hit
GTTATATAATTTAAGCTCCATAGGG13930.2261040630559271No Hit
GACCTGGATTGCTCCGGTCTGAACT13530.21961148407370376No Hit
ATCCTGACCGTGCAAAGGTAGCATA13300.21587825115892534No Hit
GATAGAAACCGACCTGGATTGCTCC13160.21360584851514716No Hit
CCTCTAGCATTACAAGTATTAGAGG12890.20922335770214648No Hit
GTTATAGATTAACCCAATTTTAAGT12410.2014322629234785No Hit
GTCCTGATCCAACATCGAGGTCGTA12360.20062069055070053No Hit
CTGTTAGTATGAGTAACAAGAATTC12320.19997143265247821No Hit
ATCTATAACTTTATAGATGCAACAC12170.19753671553414445No Hit
GTATCCTGACCGTGCAAAGGTAGCA11870.19266728129747698No Hit
ACCTATAACTTCTCTGTTAACCCAA11840.19218033787381025No Hit
TGCTAGAGGTGATGTTTTTGGTAAA11740.19055719312825442No Hit
ATAAATAATCCACCTATAACTTCTC11410.18520081546792017No Hit
GATTAAACCTTGTACCTTTTGCATA11290.18325304177325316No Hit
CTATAGAACTAGTACCGCAAGGGAA11180.18146758255314174No Hit
ATTTCAGCCTCTTCACTGAAAGGTC10780.17497500357091844No Hit
AGGGATAACAGCGCAATCCTATTTA10750.17448806014725168No Hit
GGATTGCTCCGGTCTGAACTCAGAT10680.1733518588253626No Hit
ATTATAACCTAGACTTACAAGTCAA10660.17302722987625144No Hit
CCTATAACTTCTCTGTTAACCCAAC10510.1705925127579177No Hit
ATTTAAGAGTTCATATCGACAATTA10500.17043019828336212No Hit
CTGTTAACCCAACACCGGAATGCCT10410.16896936801236187No Hit
TATCAACGCAGAGTACATGGGAGAA10380.1684824245886951No Hit
AAATATATCTGGGTCAATAAGATAT10010.16247678903013857No Hit
GGTCAGGATACCGCGGCCGTTAAAC9840.15971744296269366No Hit
CACTATAAATAATCCACCTATAACT9470.1537118074041371No Hit
GCAGAGTACTTTTTTTTTTTTTTTT9260.15030320343846984No Hit
GTTTAAAATTGAACTTAAATTCATT9180.14900468764202515No Hit
CCTATAGTCTGATTAACTAACAATG9130.14819311526924725No Hit
GTTAGTATGAGTAACAAGAATTCCA9020.14640765604913583No Hit
AGCATGAACGGCTAAACGAGGGTCC9020.14640765604913583No Hit
CTATAGTATAAGTTTGAAATTTCGG8970.14559608367635793No Hit
GCTATCACCAAGCTCGTTAGGCTTT8830.14332368103257975No Hit
GTATAAGTTTGAAATTTCGGTTGGG8560.138941190219579No Hit
CTTCACTGAAAGGTCAATTTCACTG8520.13829193232135667No Hit
GACTATAGGCAATAATCACACTATA8480.13764267442313435No Hit
CTATTAAAGGTTTTTTCCGTTCCAG8300.13472101388113386No Hit
GTTATCCCTAGGGTAACTTGGTCCG8150.1322862967628001No Hit
ACCTTTGCACGGTCAGGATACCGCG7970.12936463622079963No Hit
CCATTAATAGCTTCTACACCATTGG7930.1287153783225773No Hit
TACTAACAGTGTTGCATCTATAAAG7820.12692991910246587No Hit
CTTTATTGGTGGCTGCTTTTAGGCC7820.12692991910246587No Hit
GGGCAGGCAGTGCCTCTAATACTTG7760.12595603225513238No Hit
GGTATCAACGCAGAGTACATGGGAG7590.12319668618768748No Hit
AAGTTACCCTAGGGATAACAGCGCA7570.12287205723857632No Hit
CAATTAGGGTTTACGACCTCGATGT7530.12222279934035397No Hit
CCTTTAGGCATTCCGGTGTTGGGTT7500.12173585591668723No Hit
TTATTATATTTCAGCCTCTTCACTG7430.12059965459479814No Hit
AAACTATAGTATAAGTTTGAAATTT7430.12059965459479814No Hit
GATAAAAGGAACTCGGCAAACAAGA7260.11784030852735324No Hit
ACGCAGAGTACATGGGAGAAATCGT7200.11686642168001973No Hit
ACTAAAAGTATTGGAGAAAGAAATT7170.11637947825635299No Hit
CTTAAATAGGATTGCGCTGTTATCC7080.11491864798535273No Hit
GATCAGGACATCCCAATGGTGTAGA7080.11491864798535273No Hit
ACCCTAGGGATAACAGCGCAATCCT7050.11443170456168598No Hit
AATCTAAACTTACTTTTTGATTTTG6980.1132955032397969No Hit
GGTCTGAACTCAGATCACGTAGGAC6960.11297087429068574No Hit
ATCAACGCAGAGTACTTTTTTTTTT6940.11264624534157458No Hit
ATTTTAAACTTGCTAAAAAAACAAC6870.1115100440196855No Hit
AACCTAGACTTACAAGTCAAAGTAA6700.10875069795224059No Hit
GTTATACGCGTATGCCTGGAGAATT6650.10793912557946267No Hit
GTTTAGATTATAGCCAAAAGAGGGA6620.10745218215579593No Hit
ATCTTATTGACCCAGATATATTTTG6620.10745218215579593No Hit
GATTATAACCTAGACTTACAAGTCA6560.10647829530846242No Hit
CTCTTAAATAGGATTGCGCTGTTAT6450.104692836088351No Hit
GGGATGTCCTGATCCAACATCGAGG6440.10453052161379543No Hit
TCTAGGAGCTATAGAACTAGTACCG6360.10323200581735077No Hit
CAGTTGGACCCTCGTTTAGCCGTTC6260.10160886107179494No Hit
CATTTATCCTACTAAAAGTATTGGA6260.10160886107179494No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGT307.726145E-418.9961011
TGTAGTG250.00603801318.9945565
AATAACC350.002178252416.2744392
TCCAACG508.685408E-515.20305518
ATGTCCA508.691432E-515.2018219
CGAGTGA456.7438354E-414.78074714
GGTATCA7450.014.5339291
CGACAAG400.005267279714.25286515
TCGTCCC400.005267279714.25286513
AAATGTC602.5623598E-514.2505497
AGCGTTC1301.8189894E-1213.88740612
AAAGCGT1301.8189894E-1213.88627810
ATTGAAC1750.013.5730548
AATGTCC707.236551E-613.5730538
GTATAGT707.260891E-613.5686431
AAGCGTT1351.8189894E-1213.37197211
CCAACGA500.001495485413.30267319
TCGTGTT655.4317992E-513.1564917
CGTTCCA2000.012.82757918
TGAGTTA1501.8189894E-1212.66921312
Produced by FastQC (version 0.11.5)