##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4064161_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 616088 Sequences flagged as poor quality 0 Sequence length 25 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.0245987586189 32.0 32.0 32.0 21.0 32.0 2 28.584573956967187 32.0 32.0 32.0 14.0 32.0 3 28.650100310345277 32.0 32.0 32.0 14.0 32.0 4 28.63349553959824 32.0 32.0 32.0 14.0 32.0 5 28.37542364077859 32.0 21.0 32.0 14.0 32.0 6 31.52451760138162 36.0 32.0 36.0 21.0 36.0 7 31.720710353066444 36.0 32.0 36.0 21.0 36.0 8 31.45153776733194 36.0 32.0 36.0 21.0 36.0 9 32.27989183363415 36.0 32.0 36.0 21.0 36.0 10 30.6721702094506 36.0 27.0 36.0 14.0 36.0 11 32.23043461323707 36.0 32.0 36.0 21.0 36.0 12 31.257601186843438 36.0 32.0 36.0 21.0 36.0 13 31.825210034930073 36.0 32.0 36.0 21.0 36.0 14 31.245497396475827 36.0 32.0 36.0 14.0 36.0 15 31.18230512524185 36.0 32.0 36.0 14.0 36.0 16 31.19789380737817 36.0 32.0 36.0 14.0 36.0 17 31.02225980704055 36.0 32.0 36.0 14.0 36.0 18 31.108859448650193 36.0 32.0 36.0 14.0 36.0 19 31.103504694134603 36.0 32.0 36.0 14.0 36.0 20 30.833307904065652 36.0 32.0 36.0 14.0 36.0 21 30.975813520146474 36.0 32.0 36.0 14.0 36.0 22 30.791700211658075 36.0 32.0 36.0 14.0 36.0 23 30.755809235044346 36.0 32.0 36.0 14.0 36.0 24 30.51972607809274 36.0 27.0 36.0 14.0 36.0 25 29.330129786653856 32.0 21.0 36.0 14.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 6.0 5 16.0 6 27.0 7 15.0 8 37.0 9 48.0 10 46.0 11 24.0 12 36.0 13 24.0 14 411.0 15 824.0 16 1183.0 17 1513.0 18 1898.0 19 2804.0 20 3945.0 21 5382.0 22 7523.0 23 10373.0 24 13907.0 25 18770.0 26 24650.0 27 31703.0 28 40564.0 29 49335.0 30 58041.0 31 68309.0 32 77398.0 33 77105.0 34 76340.0 35 43828.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.8043039491567 23.858777987604185 13.19454480208751 23.142373261151604 2 14.028945138638644 22.58003252919349 41.93457801325191 21.456444318915953 3 15.393854988905609 27.894078175293025 34.12316966872647 22.588897167074894 4 9.305926867394195 18.994122316206727 43.922416521794176 27.777534294604898 5 9.595125007710513 41.32591609013671 37.581853185334765 11.497105716818009 6 25.34779827862173 44.57726884604021 19.140674460811212 10.934258414526848 7 24.595223367864964 37.21948123002093 22.76473844765138 15.42055695446273 8 24.397740501941094 35.76098939906379 21.375117918975658 18.466152180019453 9 25.361233895090336 16.304972669964243 24.156146578630285 34.177646856315135 10 13.774589651437175 28.150390494951743 35.805845487749224 22.269174365861858 11 29.775952397192423 26.143055271354843 27.603034938823768 16.47795739262897 12 21.521598256353123 30.122084515551688 31.3023268655771 17.053990362518086 13 23.459465078393123 24.457874964064068 29.957818928370138 22.12484102917267 14 19.683705489108192 26.053123964579694 29.775114830142346 24.488055716169775 15 21.60078085286413 31.13748239914119 27.639885210731208 19.621851537263474 16 21.389754200868598 31.335247666905957 27.258139436679198 20.01685869554625 17 18.035308256999937 29.68800753589294 30.43201455206912 21.844669655038004 18 17.93036565099653 29.10100440717559 32.82314278891529 20.145487152912587 19 20.53110081266741 29.099763330718115 31.98024128246487 18.388894574149607 20 20.05185235723545 30.05324938189781 31.63659814748032 18.25830011338642 21 22.228322399037108 27.712146733436533 30.555821761496443 19.503709106029916 22 20.246845796100434 29.700766885366804 30.515409993713522 19.536977324819244 23 20.896877741317134 28.809577958998016 31.307709802137822 18.98583449754703 24 19.80959837309103 30.45272770390777 30.519811709976185 19.217862213025015 25 19.71432655726627 29.334728183002547 32.46453042407545 18.486414835655737 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 109.0 1 109.0 2 376.5 3 644.0 4 644.0 5 644.0 6 2258.0 7 3872.0 8 3872.0 9 3872.0 10 3903.5 11 3935.0 12 3935.0 13 3935.0 14 4864.5 15 5794.0 16 5794.0 17 5794.0 18 9719.0 19 13644.0 20 13644.0 21 13644.0 22 21161.5 23 28679.0 24 28679.0 25 28679.0 26 41747.5 27 54816.0 28 54816.0 29 54816.0 30 62762.0 31 70708.0 32 70708.0 33 70708.0 34 76515.0 35 82322.0 36 82322.0 37 82322.0 38 77952.0 39 73582.0 40 73582.0 41 73582.0 42 67874.0 43 62166.0 44 62166.0 45 62166.0 46 68966.5 47 75767.0 48 75767.0 49 75767.0 50 68104.0 51 60441.0 52 60441.0 53 60441.0 54 50032.0 55 39623.0 56 39623.0 57 39623.0 58 29618.0 59 19613.0 60 19613.0 61 19613.0 62 14897.5 63 10182.0 64 10182.0 65 10182.0 66 7769.0 67 5356.0 68 5356.0 69 5356.0 70 3937.0 71 2518.0 72 2518.0 73 2518.0 74 1928.5 75 1339.0 76 1339.0 77 1339.0 78 929.5 79 520.0 80 520.0 81 520.0 82 343.5 83 167.0 84 167.0 85 167.0 86 114.5 87 62.0 88 62.0 89 62.0 90 43.0 91 24.0 92 24.0 93 24.0 94 20.0 95 16.0 96 16.0 97 16.0 98 102.5 99 189.0 100 189.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.038143901520562 2 0.004220176338445157 3 0.0017854592201114127 4 0.004707119762111906 5 0.007466465829556817 6 0.011362013218890809 7 0.021263196166781368 8 0.03230058043656101 9 0.045772681824674394 10 0.05372609107789796 11 0.052914518705120046 12 0.059569412161898955 13 0.06638662009323343 14 0.06427653192401085 15 0.05697238056900962 16 0.062004129280232696 17 0.059731726636454534 18 0.08131955175234706 19 0.07482697277012375 20 0.08018335043045798 21 0.07158068327901208 22 0.0780732622612354 23 0.08245575307423615 24 0.07174299775356767 25 0.07953409253223566 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 616088.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.75501854623514 #Duplication Level Percentage of deduplicated Percentage of total 1 61.627791359397875 13.407137439872102 2 15.451410509174144 6.72291444385152 3 7.213109103196784 4.707639669481907 4 3.9452029496856786 3.433118533562941 5 2.489481565336295 2.707935881220079 6 1.6809547366212954 2.1941520882346848 7 1.2125705173221772 1.8465645865073332 8 0.8658082302346344 1.5068539284989995 9 0.6799647330541141 1.331338084054027 >10 3.912297654508502 16.132954793517733 >50 0.39882979222139825 6.1574309509381875 >100 0.4301397946013959 19.50618076369061 >500 0.058892623524281235 8.694875696595703 >1k 0.03280095487428322 10.738811279461718 >5k 7.454762471428005E-4 0.9120918605124548 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5624 0.9128566049005986 No Hit TATCAACGCAGAGTACTTTTTTTTT 3880 0.6297801612756619 No Hit CCATAGGGTCTTCTCGTCTTATTAT 3311 0.5374232252535353 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2385 0.3871200218150654 No Hit CCATTGGGATGTCCTGATCCAACAT 2236 0.3629351651062835 No Hit GGTATCAACGCAGAGTACTTTTTTT 2180 0.3538455545311709 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1985 0.3221942319928322 No Hit GTATTAGAGGCACTGCCTGCCCAGT 1909 0.3098583319266079 No Hit GTATCAACGCAGAGTACATGGGAGA 1767 0.2868096765397151 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1734 0.28145329887938086 No Hit TCCCAATGGTGTAGAAGCTATTAAT 1718 0.27885626728649154 No Hit CTCTAATACTTGTAATGCTAGAGGT 1699 0.27577229226993544 No Hit CTTCTACACCATTGGGATGTCCTGA 1642 0.26652036722026723 No Hit ATCGTAAATAGATAGAAACCGACCT 1597 0.259216215865266 No Hit GATATATTTTGATCAACGGACCAAG 1596 0.2590539013907104 No Hit GTGTAGAAGCTATTAATGGTTCGTT 1455 0.23616756047837323 No Hit GTACATGGGAGAAATCGTAAATAGA 1446 0.23470673020737298 No Hit GATTAAAGATAAGAGACAGTTGGAC 1442 0.23405747230915067 No Hit GCCTAAAGGAAAGATCCAAAAAGAT 1425 0.23129812624170573 No Hit GTTCATGCTAGTCCCTAATTAAGGA 1408 0.22853878017426082 No Hit GTCAGGATACCGCGGCCGTTAAACT 1393 0.2261040630559271 No Hit GTTATATAATTTAAGCTCCATAGGG 1393 0.2261040630559271 No Hit GACCTGGATTGCTCCGGTCTGAACT 1353 0.21961148407370376 No Hit ATCCTGACCGTGCAAAGGTAGCATA 1330 0.21587825115892534 No Hit GATAGAAACCGACCTGGATTGCTCC 1316 0.21360584851514716 No Hit CCTCTAGCATTACAAGTATTAGAGG 1289 0.20922335770214648 No Hit GTTATAGATTAACCCAATTTTAAGT 1241 0.2014322629234785 No Hit GTCCTGATCCAACATCGAGGTCGTA 1236 0.20062069055070053 No Hit CTGTTAGTATGAGTAACAAGAATTC 1232 0.19997143265247821 No Hit ATCTATAACTTTATAGATGCAACAC 1217 0.19753671553414445 No Hit GTATCCTGACCGTGCAAAGGTAGCA 1187 0.19266728129747698 No Hit ACCTATAACTTCTCTGTTAACCCAA 1184 0.19218033787381025 No Hit TGCTAGAGGTGATGTTTTTGGTAAA 1174 0.19055719312825442 No Hit ATAAATAATCCACCTATAACTTCTC 1141 0.18520081546792017 No Hit GATTAAACCTTGTACCTTTTGCATA 1129 0.18325304177325316 No Hit CTATAGAACTAGTACCGCAAGGGAA 1118 0.18146758255314174 No Hit ATTTCAGCCTCTTCACTGAAAGGTC 1078 0.17497500357091844 No Hit AGGGATAACAGCGCAATCCTATTTA 1075 0.17448806014725168 No Hit GGATTGCTCCGGTCTGAACTCAGAT 1068 0.1733518588253626 No Hit ATTATAACCTAGACTTACAAGTCAA 1066 0.17302722987625144 No Hit CCTATAACTTCTCTGTTAACCCAAC 1051 0.1705925127579177 No Hit ATTTAAGAGTTCATATCGACAATTA 1050 0.17043019828336212 No Hit CTGTTAACCCAACACCGGAATGCCT 1041 0.16896936801236187 No Hit TATCAACGCAGAGTACATGGGAGAA 1038 0.1684824245886951 No Hit AAATATATCTGGGTCAATAAGATAT 1001 0.16247678903013857 No Hit GGTCAGGATACCGCGGCCGTTAAAC 984 0.15971744296269366 No Hit CACTATAAATAATCCACCTATAACT 947 0.1537118074041371 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 926 0.15030320343846984 No Hit GTTTAAAATTGAACTTAAATTCATT 918 0.14900468764202515 No Hit CCTATAGTCTGATTAACTAACAATG 913 0.14819311526924725 No Hit GTTAGTATGAGTAACAAGAATTCCA 902 0.14640765604913583 No Hit AGCATGAACGGCTAAACGAGGGTCC 902 0.14640765604913583 No Hit CTATAGTATAAGTTTGAAATTTCGG 897 0.14559608367635793 No Hit GCTATCACCAAGCTCGTTAGGCTTT 883 0.14332368103257975 No Hit GTATAAGTTTGAAATTTCGGTTGGG 856 0.138941190219579 No Hit CTTCACTGAAAGGTCAATTTCACTG 852 0.13829193232135667 No Hit GACTATAGGCAATAATCACACTATA 848 0.13764267442313435 No Hit CTATTAAAGGTTTTTTCCGTTCCAG 830 0.13472101388113386 No Hit GTTATCCCTAGGGTAACTTGGTCCG 815 0.1322862967628001 No Hit ACCTTTGCACGGTCAGGATACCGCG 797 0.12936463622079963 No Hit CCATTAATAGCTTCTACACCATTGG 793 0.1287153783225773 No Hit TACTAACAGTGTTGCATCTATAAAG 782 0.12692991910246587 No Hit CTTTATTGGTGGCTGCTTTTAGGCC 782 0.12692991910246587 No Hit GGGCAGGCAGTGCCTCTAATACTTG 776 0.12595603225513238 No Hit GGTATCAACGCAGAGTACATGGGAG 759 0.12319668618768748 No Hit AAGTTACCCTAGGGATAACAGCGCA 757 0.12287205723857632 No Hit CAATTAGGGTTTACGACCTCGATGT 753 0.12222279934035397 No Hit CCTTTAGGCATTCCGGTGTTGGGTT 750 0.12173585591668723 No Hit TTATTATATTTCAGCCTCTTCACTG 743 0.12059965459479814 No Hit AAACTATAGTATAAGTTTGAAATTT 743 0.12059965459479814 No Hit GATAAAAGGAACTCGGCAAACAAGA 726 0.11784030852735324 No Hit ACGCAGAGTACATGGGAGAAATCGT 720 0.11686642168001973 No Hit ACTAAAAGTATTGGAGAAAGAAATT 717 0.11637947825635299 No Hit CTTAAATAGGATTGCGCTGTTATCC 708 0.11491864798535273 No Hit GATCAGGACATCCCAATGGTGTAGA 708 0.11491864798535273 No Hit ACCCTAGGGATAACAGCGCAATCCT 705 0.11443170456168598 No Hit AATCTAAACTTACTTTTTGATTTTG 698 0.1132955032397969 No Hit GGTCTGAACTCAGATCACGTAGGAC 696 0.11297087429068574 No Hit ATCAACGCAGAGTACTTTTTTTTTT 694 0.11264624534157458 No Hit ATTTTAAACTTGCTAAAAAAACAAC 687 0.1115100440196855 No Hit AACCTAGACTTACAAGTCAAAGTAA 670 0.10875069795224059 No Hit GTTATACGCGTATGCCTGGAGAATT 665 0.10793912557946267 No Hit GTTTAGATTATAGCCAAAAGAGGGA 662 0.10745218215579593 No Hit ATCTTATTGACCCAGATATATTTTG 662 0.10745218215579593 No Hit GATTATAACCTAGACTTACAAGTCA 656 0.10647829530846242 No Hit CTCTTAAATAGGATTGCGCTGTTAT 645 0.104692836088351 No Hit GGGATGTCCTGATCCAACATCGAGG 644 0.10453052161379543 No Hit TCTAGGAGCTATAGAACTAGTACCG 636 0.10323200581735077 No Hit CAGTTGGACCCTCGTTTAGCCGTTC 626 0.10160886107179494 No Hit CATTTATCCTACTAAAAGTATTGGA 626 0.10160886107179494 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.6231447455558297E-4 0.0 8 0.0 0.0 0.0 1.6231447455558297E-4 0.0 9 0.0 0.0 0.0 1.6231447455558297E-4 0.0 10 0.0 0.0 0.0 1.6231447455558297E-4 0.0 11 0.0 0.0 0.0 1.6231447455558297E-4 0.0 12 0.0 0.0 0.0 1.6231447455558297E-4 0.0 13 0.0 0.0 0.0 1.6231447455558297E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTTGT 30 7.726145E-4 18.996101 1 TGTAGTG 25 0.006038013 18.994556 5 AATAACC 35 0.0021782524 16.274439 2 TCCAACG 50 8.685408E-5 15.203055 18 ATGTCCA 50 8.691432E-5 15.201821 9 CGAGTGA 45 6.7438354E-4 14.780747 14 GGTATCA 745 0.0 14.533929 1 CGACAAG 40 0.0052672797 14.252865 15 TCGTCCC 40 0.0052672797 14.252865 13 AAATGTC 60 2.5623598E-5 14.250549 7 AGCGTTC 130 1.8189894E-12 13.887406 12 AAAGCGT 130 1.8189894E-12 13.886278 10 ATTGAAC 175 0.0 13.573054 8 AATGTCC 70 7.236551E-6 13.573053 8 GTATAGT 70 7.260891E-6 13.568643 1 AAGCGTT 135 1.8189894E-12 13.371972 11 CCAACGA 50 0.0014954854 13.302673 19 TCGTGTT 65 5.4317992E-5 13.15649 17 CGTTCCA 200 0.0 12.827579 18 TGAGTTA 150 1.8189894E-12 12.669213 12 >>END_MODULE