FastQCFastQC Report
Thu 2 Feb 2017
SRR4064161_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064161_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences616088
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG28640.4648686551271896No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT22530.3656945111737284No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA21640.3512485229382815No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT17920.2908675384036047No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA16630.26992897118593445No Hit
TCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGT16480.26749425406760075No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC16390.2660334237966005No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGATAGAAACCGACC16140.26197556193271093No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT14540.23600524600381764No Hit
GTGTAGAAGCTATTAATGGTTCGTTTGTTCAACGATTAAAGTCCTACGTG14010.22740257885237175No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC13890.22545480515770477No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA13830.22448091831037123No Hit
AGCATGAACGGCTAAACGAGGGTCCAACTGTCTCTTATCTTTAATCAGTG13510.2192868551245926No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCAC13040.21165807482048019No Hit
GACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTT12880.20906104322759086No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGG12800.2077625274311462No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGAC12780.20743789848203503No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGAT12660.20549012478736803No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC12360.20062069055070053No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTT12120.19672514316136655No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC11980.19445274051758837No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA11950.19396579709392164No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA11450.18585007336614248No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC11300.18341535624780875No Hit
AGGGATAACAGCGCAATCCTATTTAAGAGTTCATATCGACAATTAGGGTT11230.18227915492591967No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC11000.17854592201114128No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGT10900.17692277726558545No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCTTGTTTGCCGAGT10890.17676046279102986No Hit
ATTTAAGAGTTCATATCGACAATTAGGGTTTACGACCTCGATGTTGGATC10620.17237797197802912No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC10570.1715663996052512No Hit
GGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA10390.1686447390632507No Hit
ATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGAC10280.16685927984313928No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC10180.16523613509758345No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGATATGAACTCT10100.16393761930113881No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA9900.16069132981002715No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA9820.15939281401358246No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGATAAAA9760.15841892716624897No Hit
GTTATCCCTAGGGTAACTTGGTCCGTTGATCAAAATATATCTGGGTCAAT9520.154523379776915No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG9380.15225097713313682No Hit
TATCAACGCAGAGTACATGGGAGAAATCGTAAATAGATAGAAACCGACCT9150.14851774421835842No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG9140.14835542974380284No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG9030.14656997052369142No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA8530.13845424679591228No Hit
CTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGGTCCGTTGATCA8410.13650647310124528No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT8290.13455869940657827No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT8170.1326109257119113No Hit
GGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCT8060.13082546649179988No Hit
CAATTAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAATGGTGTA7990.12968926516991078No Hit
CTATAGTATAAGTTTGAAATTTCGGTTGGGGTGACCTCGGAGAATAAAAA7610.12352131513679865No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA7490.12157354144213164No Hit
GGGCAGGCAGTGCCTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGT7460.12108659801846489No Hit
GTATAAGTTTGAAATTTCGGTTGGGGTGACCTCGGAGAATAAAAAATCCT7440.12076196906935374No Hit
CCATTAATAGCTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTC7300.11848956642557557No Hit
AAGTTACCCTAGGGATAACAGCGCAATCCTATTTAAGAGTTCATATCGAC7270.11800262300190882No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA7250.11767799405279765No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT7230.11735336510368649No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT7220.11719105062913089No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT7100.11524327693446391No Hit
ACGCAGAGTACATGGGAGAAATCGTAAATAGATAGAAACCGACCTGGATT6970.11313318876524134No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA6930.112483930867019No Hit
GATCAGGACATCCCAATGGTGTAGAAGCTATTAATGGTTCGTTTGTTCAA6750.1095622703250185No Hit
GATAAAAGGAACTCGGCAAACAAGAACCCCGCCTGTTTACCAAAAACATC6740.10939995585046293No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTC6720.10907532690135174No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT6600.10712755320668477No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6450.104692836088351No Hit
CAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTCCCTAATTAAGGAACAA6380.10355663476646193No Hit
TCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTA6370.10339432029190636No Hit
CCAATAAAGAAAGCGTTCAAGCTCAACATAAAATTTCAATTAATTCCATA6340.10290737686823961No Hit
ATTTCAGCCTCTTCACTGAAAGGTCAATTTCACTGATTAAAGATAAGAGA6220.1009596031735726No Hit
CTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTATCCTGACCGTGCA6170.1001480308007947No Hit
GTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACTAGCATGAAC6170.1001480308007947No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCCGG250.00235213435.19928744
CGACATG250.00235307135.1964315
CCGACAT250.00235307135.1964314
ATTTGCG300.00574114629.33511731
GTCCGAC300.00574570329.33035912
TATCGTT300.00574570329.33035913
GCTATCG554.959782E-627.9971613
GCCTCGT407.0323603E-427.497217
TACGGCC508.336061E-526.39946419
TTGTGCG609.750522E-625.6640627
GCGCTAT609.750522E-625.66406211
ACAACCG450.00139826324.44196532
CAACCGT450.00139826324.44196533
GGTAACC2000.024.20147331
CTACTTG651.8129425E-523.6899033
TGCGCTA651.8129425E-523.68990310
GGCTCGC651.8129425E-523.68990317
GACGTGA1500.023.4642857
GAACATG602.8678065E-422.00312426
CGCGCCA703.210544E-522.00133921
Produced by FastQC (version 0.11.5)