FastQCFastQC Report
Thu 2 Feb 2017
SRR4064160_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064160_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3397025
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67490.19867383960965845No Hit
TATCAACGCAGAGTACTTTTTTTTT44350.13055541245648766No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACACG456.77667E-414.774494
CGTCTTA1600.013.06555915
GTATCAA40550.012.9285451
GGTATCA14900.012.2381671
TCGCGTA2250.011.8229019
GTCCTAA2950.011.5899591
CGGTCCA5100.011.54934110
ACTCTAA4950.011.13162310
CGTTATT1658.185452E-1110.9347062
GTATTAG4050.010.7870881
CGCATCG4500.010.76760213
AACCGCG2500.010.640147
GTATTAT2500.010.6370071
CTAACAC3050.010.586563
CCGTCGT4150.010.53072559
CTTAATC6350.010.4688783
AGGCCCG6450.010.45765610
CACTCTA5550.010.442049
CGAGCCG6100.010.43687115
CGAACGA2200.010.36591116
Produced by FastQC (version 0.11.5)