##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064160_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3397025
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.263867648898668	32.0	32.0	32.0	32.0	32.0
2	30.829741612145924	32.0	32.0	32.0	32.0	32.0
3	30.851833295309866	32.0	32.0	32.0	32.0	32.0
4	30.88365820092581	32.0	32.0	32.0	32.0	32.0
5	30.77269610909546	32.0	32.0	32.0	32.0	32.0
6	34.439751252934556	36.0	36.0	36.0	32.0	36.0
7	34.38381377823242	36.0	36.0	36.0	32.0	36.0
8	34.33680264348953	36.0	36.0	36.0	32.0	36.0
9	34.499569770608105	36.0	36.0	36.0	32.0	36.0
10	34.16904261817326	36.0	36.0	36.0	32.0	36.0
11	34.4713871696558	36.0	36.0	36.0	32.0	36.0
12	34.27161648795637	36.0	36.0	36.0	32.0	36.0
13	34.36200322340872	36.0	36.0	36.0	32.0	36.0
14	34.25559482193979	36.0	36.0	36.0	32.0	36.0
15	34.184419602446255	36.0	36.0	36.0	32.0	36.0
16	34.18373253067022	36.0	36.0	36.0	32.0	36.0
17	34.108595020643065	36.0	36.0	36.0	32.0	36.0
18	34.09972196259963	36.0	36.0	36.0	32.0	36.0
19	34.127835090998744	36.0	36.0	36.0	32.0	36.0
20	34.106925913115155	36.0	36.0	36.0	32.0	36.0
21	34.095955137215654	36.0	36.0	36.0	32.0	36.0
22	34.083404449481534	36.0	36.0	36.0	32.0	36.0
23	34.012521250211584	36.0	36.0	36.0	32.0	36.0
24	33.99310749847293	36.0	36.0	36.0	32.0	36.0
25	33.58709105761659	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	12.0
4	26.0
5	42.0
6	133.0
7	50.0
8	194.0
9	225.0
10	300.0
11	106.0
12	211.0
13	186.0
14	297.0
15	563.0
16	871.0
17	1154.0
18	1567.0
19	2132.0
20	3199.0
21	4967.0
22	7675.0
23	12137.0
24	18324.0
25	26900.0
26	38446.0
27	50814.0
28	68803.0
29	92680.0
30	122785.0
31	166022.0
32	235572.0
33	333465.0
34	708206.0
35	1498961.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.781771480755395	16.857040962941433	11.180562120271086	26.180625436032084
2	17.110023816463094	19.214881020722384	36.8749503210365	26.800144841778025
3	18.866843098408285	23.04483112891783	28.54956034348257	29.53876542919131
4	12.768336966553278	15.08380447438028	35.357848859031556	36.79000970003489
5	14.836383527186783	36.18321653451973	33.67609496679742	15.304304971496066
6	35.06404111444258	35.01672865080281	16.434942565554405	13.484287669200205
7	30.7817784271153	30.23432683151752	20.407580995924366	18.57631374544281
8	28.62990821921963	32.59935997578191	18.96938255361241	19.801349251386046
9	27.278400765747524	13.98888193498509	18.173839413908627	40.55887788535876
10	15.740385651951852	26.55744397337089	31.56583552326571	26.13633485141154
11	37.486407040652225	20.91280477388267	22.17304735592641	19.427740829538692
12	24.614633258347933	23.341305134816135	28.639455321435232	23.404606285400707
13	29.557941395834387	19.187126124204823	25.157474098957124	26.097458381003662
14	23.655367278237375	19.14974154254385	24.957670006327408	32.23722117289137
15	25.308786160300468	26.794447224687456	21.984001706044825	25.91276490896725
16	26.006131759782065	25.775041946704118	23.156200806884915	25.062625486628896
17	24.396196881320144	25.817682887674387	24.797151490265687	24.988968740739782
18	25.076913513652055	24.941130928409375	25.517197899951004	24.46475765798756
19	25.743326784510135	24.754787372407684	25.135019765780147	24.36686607730204
20	25.8783136636914	24.281055658619984	24.59251743428796	25.24811324340066
21	26.80501022832256	24.062364486509928	24.35987551612964	24.772749769037876
22	26.191843294146068	23.853628860603614	24.65627794136493	25.298249903885388
23	24.829078588789866	23.881417102452676	25.22330411725713	26.066200191500332
24	24.98699336735385	24.67238899582904	24.947487377821613	25.393130258995495
25	25.13293284817889	24.266560373416908	24.94800969719681	25.652497081207393
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	207.0
1	207.0
2	475.0
3	743.0
4	743.0
5	743.0
6	2133.5
7	3524.0
8	3524.0
9	3524.0
10	4215.5
11	4907.0
12	4907.0
13	4907.0
14	6693.0
15	8479.0
16	8479.0
17	8479.0
18	14646.0
19	20813.0
20	20813.0
21	20813.0
22	31776.5
23	42740.0
24	42740.0
25	42740.0
26	63133.5
27	83527.0
28	83527.0
29	83527.0
30	106312.0
31	129097.0
32	129097.0
33	129097.0
34	163291.0
35	197485.0
36	197485.0
37	197485.0
38	232728.5
39	267972.0
40	267972.0
41	267972.0
42	310056.0
43	352140.0
44	352140.0
45	352140.0
46	395118.0
47	438096.0
48	438096.0
49	438096.0
50	453970.0
51	469844.0
52	469844.0
53	469844.0
54	444437.0
55	419030.0
56	419030.0
57	419030.0
58	385603.0
59	352176.0
60	352176.0
61	352176.0
62	308942.0
63	265708.0
64	265708.0
65	265708.0
66	217107.0
67	168506.0
68	168506.0
69	168506.0
70	126817.5
71	85129.0
72	85129.0
73	85129.0
74	64541.0
75	43953.0
76	43953.0
77	43953.0
78	34205.0
79	24457.0
80	24457.0
81	24457.0
82	16819.5
83	9182.0
84	9182.0
85	9182.0
86	6940.5
87	4699.0
88	4699.0
89	4699.0
90	3334.5
91	1970.0
92	1970.0
93	1970.0
94	1410.0
95	850.0
96	850.0
97	850.0
98	1320.5
99	1791.0
100	1791.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0396229053362869
2	0.006329067345692186
3	0.0016190637395956757
4	0.0038268779299534153
5	0.00886069428396906
6	0.013452947799913158
7	0.021872079245810673
8	0.03579602740633348
9	0.04857191218787027
10	0.0571971062915345
11	0.055489729984324526
12	0.06305517327661704
13	0.06420323665560305
14	0.06679373863895614
15	0.05999367093265431
16	0.06596948800788925
17	0.06261361043854549
18	0.07736180923013519
19	0.07424143184109626
20	0.07971681103318345
21	0.07444749449886297
22	0.07771505950059243
23	0.08168912504323636
24	0.07662587116668261
25	0.07777393454566864
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3397025.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.63818986376444
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.04369448518538	34.53263881614644
2	13.968469274775918	13.029282442863185
3	4.784793531353796	6.694623275225709
4	2.188064566057953	4.081894826639447
5	1.1983017977019939	2.7943313377657915
6	0.7472454898243234	2.0910106217561073
7	0.5180307573584203	1.6912011771866182
8	0.37966327321322113	1.4165446256333183
9	0.28719689981064483	1.2054909187488048
>10	1.6835737995364535	14.430010764174138
>50	0.11325465441500132	3.637554624403088
>100	0.0717252279083473	6.681110274577555
>500	0.01059405776884987	3.428406346888905
>1k	0.005328747618139718	4.086223436904508
>5k	6.343747164452046E-5	0.19967651108634613
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6749	0.19867383960965845	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4435	0.13055541245648766	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.8875045076206386E-5
2	0.0	0.0	0.0	0.0	5.8875045076206386E-5
3	0.0	0.0	0.0	0.0	5.8875045076206386E-5
4	0.0	0.0	0.0	0.0	5.8875045076206386E-5
5	0.0	2.9437522538103193E-5	0.0	0.0	8.831256761430958E-5
6	0.0	5.8875045076206386E-5	0.0	0.0	1.1775009015241277E-4
7	0.0	5.8875045076206386E-5	0.0	0.0	1.7662513522861915E-4
8	0.0	5.8875045076206386E-5	0.0	0.0	1.7662513522861915E-4
9	0.0	5.8875045076206386E-5	0.0	2.9437522538103193E-5	1.7662513522861915E-4
10	0.0	5.8875045076206386E-5	0.0	2.9437522538103193E-5	1.7662513522861915E-4
11	0.0	5.8875045076206386E-5	0.0	2.9437522538103193E-5	1.7662513522861915E-4
12	0.0	8.831256761430958E-5	0.0	2.9437522538103193E-5	3.2381274791913514E-4
13	0.0	8.831256761430958E-5	0.0	2.9437522538103193E-5	3.532502704572383E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACACG	45	6.77667E-4	14.77449	4
CGTCTTA	160	0.0	13.065559	15
GTATCAA	4055	0.0	12.928545	1
GGTATCA	1490	0.0	12.238167	1
TCGCGTA	225	0.0	11.822901	9
GTCCTAA	295	0.0	11.589959	1
CGGTCCA	510	0.0	11.549341	10
ACTCTAA	495	0.0	11.131623	10
CGTTATT	165	8.185452E-11	10.934706	2
GTATTAG	405	0.0	10.787088	1
CGCATCG	450	0.0	10.767602	13
AACCGCG	250	0.0	10.64014	7
GTATTAT	250	0.0	10.637007	1
CTAACAC	305	0.0	10.58656	3
CCGTCGT	415	0.0	10.5307255	9
CTTAATC	635	0.0	10.468878	3
AGGCCCG	645	0.0	10.457656	10
CACTCTA	555	0.0	10.44204	9
CGAGCCG	610	0.0	10.436871	15
CGAACGA	220	0.0	10.365911	16
>>END_MODULE