FastQCFastQC Report
Thu 2 Feb 2017
SRR4064160_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064160_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3397025
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44150.026.5597761
GGTATCA18650.022.177211
GTATCGC1152.0260268E-719.1286956
AACGCAG62400.018.6489436
TCAACGC64200.018.1260764
CAACGCA65250.017.8681075
ATCAACG65700.017.7459813
TATCAAC66650.017.6583272
CGACGGT8500.017.33967
ACGACGG9450.016.0620336
GACGGTA9600.016.0404438
CTAGATA7550.016.0252693
TAGGCCG1105.6304307E-515.9985455
TACGACG9500.015.9774935
ACGGTAT9550.015.8947769
TATATCG850.002983235315.5279985
ACGCAGA75150.015.4849517
TCTAGAT9150.015.3870312
GTATTAG8700.015.1725971
AGAGTAC77300.015.11228611
Produced by FastQC (version 0.11.5)