##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064160_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3397025
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.351228207034097	32.0	32.0	32.0	32.0	32.0
2	31.42452204502469	32.0	32.0	32.0	32.0	32.0
3	31.4956819570065	32.0	32.0	32.0	32.0	32.0
4	31.575821196488103	32.0	32.0	32.0	32.0	32.0
5	31.52923484519543	32.0	32.0	32.0	32.0	32.0
6	35.12607148902348	36.0	36.0	36.0	36.0	36.0
7	35.14520705617415	36.0	36.0	36.0	36.0	36.0
8	35.08825781382239	36.0	36.0	36.0	36.0	36.0
9	35.202108609739405	36.0	36.0	36.0	36.0	36.0
10	35.05797896689015	36.0	36.0	36.0	36.0	36.0
11	35.1942043405627	36.0	36.0	36.0	36.0	36.0
12	35.10689088246333	36.0	36.0	36.0	36.0	36.0
13	35.14297510321531	36.0	36.0	36.0	36.0	36.0
14	35.08570705249446	36.0	36.0	36.0	36.0	36.0
15	35.06414671661233	36.0	36.0	36.0	36.0	36.0
16	35.07675686814198	36.0	36.0	36.0	36.0	36.0
17	35.03962997034169	36.0	36.0	36.0	36.0	36.0
18	35.03922432128112	36.0	36.0	36.0	36.0	36.0
19	35.035529617827365	36.0	36.0	36.0	36.0	36.0
20	35.032636792487544	36.0	36.0	36.0	36.0	36.0
21	35.02820938909781	36.0	36.0	36.0	36.0	36.0
22	35.0158002958471	36.0	36.0	36.0	36.0	36.0
23	34.96285308468439	36.0	36.0	36.0	36.0	36.0
24	34.93576585394573	36.0	36.0	36.0	36.0	36.0
25	34.904447273717444	36.0	36.0	36.0	32.0	36.0
26	34.85322745637727	36.0	36.0	36.0	32.0	36.0
27	34.85086597831926	36.0	36.0	36.0	32.0	36.0
28	34.82889911025088	36.0	36.0	36.0	32.0	36.0
29	34.8058872101324	36.0	36.0	36.0	32.0	36.0
30	34.79115078634982	36.0	36.0	36.0	32.0	36.0
31	34.78462507635357	36.0	36.0	36.0	32.0	36.0
32	34.751229090159775	36.0	36.0	36.0	32.0	36.0
33	34.72908677445706	36.0	36.0	36.0	32.0	36.0
34	34.72329641377382	36.0	36.0	36.0	32.0	36.0
35	34.685793598810726	36.0	36.0	36.0	32.0	36.0
36	34.667237362103606	36.0	36.0	36.0	32.0	36.0
37	34.66626710136075	36.0	36.0	36.0	32.0	36.0
38	34.62518733303405	36.0	36.0	36.0	32.0	36.0
39	34.63219846777695	36.0	36.0	36.0	32.0	36.0
40	34.61153862570926	36.0	36.0	36.0	32.0	36.0
41	34.59726849228369	36.0	36.0	36.0	32.0	36.0
42	34.53756478094804	36.0	36.0	36.0	32.0	36.0
43	34.52413773816796	36.0	36.0	36.0	32.0	36.0
44	34.47302271840802	36.0	36.0	36.0	32.0	36.0
45	34.45778673986797	36.0	36.0	36.0	32.0	36.0
46	34.43907772241888	36.0	36.0	36.0	32.0	36.0
47	34.407775627203215	36.0	36.0	36.0	32.0	36.0
48	34.3709510527594	36.0	36.0	36.0	32.0	36.0
49	34.358566098277166	36.0	36.0	36.0	32.0	36.0
50	33.76884038239342	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	3.0
19	14.0
20	32.0
21	113.0
22	324.0
23	1032.0
24	2561.0
25	5650.0
26	11461.0
27	21586.0
28	35744.0
29	54768.0
30	80351.0
31	114836.0
32	166360.0
33	271199.0
34	575269.0
35	2055718.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.977675894187485	17.698775306644386	11.71308984313244	26.610458956035686
2	16.345982881426384	19.915649400119868	36.4389645959449	27.299403122508842
3	18.14873297548965	23.567374170374332	28.893757870883075	29.390134983252942
4	12.46932242182498	15.69970783258881	35.48637410675518	36.34459563883104
5	14.397892273386272	36.633848735290435	33.484858074344466	15.483400916978827
6	35.118948442428035	35.36625357887418	16.206861429642117	13.307936549055672
7	30.75307408869472	30.3987638588258	20.386291173183107	18.46187087929637
8	28.624811415871243	32.8644328493314	18.768598994708604	19.742156740088753
9	27.410852512361707	13.824767448056113	18.039684037276608	40.72469600230558
10	15.593313841556109	26.823527195268277	31.622337199895906	25.960821763279707
11	37.72166460996449	20.830850768201813	22.106084619949698	19.341400001884
12	24.617318890474266	23.397219738835727	28.634017060251942	23.351444310438062
13	29.60497614078263	19.161674531426165	25.165336575400133	26.068012752391073
14	23.70868323590962	19.05957491528597	24.983537026750234	32.24820482205417
15	25.36939954028186	26.92686548534177	21.88303682455347	25.820698149822896
16	26.07736878654508	25.81030985253766	23.14572580595307	24.966595554964186
17	24.446891029650946	25.90605014681964	24.769408526578403	24.87765029695101
18	25.182667724458092	24.906307449334193	25.511750757650464	24.399274068557247
19	25.809657455954817	24.806602722442562	25.132917851895613	24.250821969707008
20	26.10712770726972	24.1056328637146	24.59853677247439	25.188702656541295
21	26.889822712520516	24.074123681750944	24.34465451387611	24.69139909185243
22	26.164108542192864	23.888349200181334	24.763439720696375	25.184102536929426
23	24.83171155683185	23.873835551715562	25.23550961953138	26.058943271921216
24	25.101221368203213	24.66336572456366	24.919561614478617	25.31585129275451
25	25.291076690108234	24.15214558206065	25.07447106336642	25.482306664464694
26	25.15843434163528	24.861893416603053	25.14983809919416	24.829834142567503
27	25.513842853008722	24.576609739676115	24.6014566032159	25.30809080409926
28	25.043142403786995	24.291072127740374	25.3700296447633	25.29575582370933
29	25.00185460595805	24.619570273080424	25.14621948878817	25.232355632173352
30	24.946178758059204	24.73351383161024	25.29128824487554	25.02901916545502
31	25.476694806102845	24.442066371779113	24.530650396331815	25.550588425786223
32	25.3083090662236	24.55721601593916	24.42259390576629	25.711881012070947
33	24.99571663998521	24.301812317589214	24.824912139601622	25.877558902823957
34	25.561182855588516	24.310289402319505	25.017515805438556	25.111011936653426
35	25.91977984808034	24.237465214883926	25.075281276282947	24.767473660752792
36	25.034864157114654	24.71276262197303	24.6986903677186	25.55368285319371
37	25.834448561252948	24.435737849118347	24.744370920194033	24.98544266943467
38	25.141878057145718	24.378620183086504	25.036130071200308	25.443371688567474
39	25.59406842705041	24.380098991042644	24.592853486132686	25.43297909577426
40	25.802929463492987	24.453772458474344	24.92634538890341	24.816952689129263
41	24.889590834022684	24.80598543970844	25.18294567710066	25.121478049168218
42	25.891703535491956	24.726894955567534	24.757333999027953	24.62406750991256
43	25.378807950139127	24.02916479735482	24.99717395374469	25.594853298761365
44	25.02958510206124	24.544654369469352	24.756842842760335	25.66891768570907
45	25.100663119588194	24.689738573212512	24.781673336328154	25.427924970871135
46	25.074014118982497	24.45143299047067	24.889792042183263	25.584760848363565
47	25.149766526128996	24.513637806105116	25.15409393238766	25.182501735378228
48	25.75604408414823	24.941786151021	24.39620978534324	24.90595997948753
49	25.06031040836218	24.987481634579126	24.565257260136786	25.38695069692191
50	25.088983528590276	25.048771304604063	24.6195955992637	25.242649567541964
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	42.0
1	62.0
2	82.0
3	252.5
4	423.0
5	484.5
6	546.0
7	491.0
8	436.0
9	582.5
10	729.0
11	1154.0
12	1579.0
13	2944.5
14	4310.0
15	5550.5
16	6791.0
17	6851.0
18	6911.0
19	6818.5
20	6726.0
21	7774.5
22	8823.0
23	10595.5
24	12368.0
25	15423.5
26	18479.0
27	22732.0
28	26985.0
29	31263.5
30	35542.0
31	40473.0
32	45404.0
33	52852.5
34	60301.0
35	70354.5
36	80408.0
37	95806.0
38	111204.0
39	124423.0
40	137642.0
41	152797.5
42	167953.0
43	176413.5
44	184874.0
45	203605.5
46	222337.0
47	238469.5
48	254602.0
49	263412.5
50	272223.0
51	268820.5
52	265418.0
53	263214.5
54	261011.0
55	260001.5
56	258992.0
57	250805.5
58	242619.0
59	221287.5
60	199956.0
61	175919.5
62	151883.0
63	131778.5
64	111674.0
65	95002.0
66	78330.0
67	66401.5
68	54473.0
69	48249.0
70	42025.0
71	32456.5
72	22888.0
73	19392.5
74	15897.0
75	12433.0
76	8969.0
77	7655.5
78	6342.0
79	5117.5
80	3893.0
81	3086.5
82	2280.0
83	1844.5
84	1409.0
85	1007.0
86	605.0
87	432.0
88	259.0
89	190.0
90	121.0
91	104.0
92	87.0
93	73.5
94	60.0
95	60.5
96	61.0
97	43.0
98	25.0
99	26.5
100	28.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00994988261787888
2	3.826877929953415E-4
3	3.2381274791913514E-4
4	0.0
5	0.0
6	2.0606265776672235E-4
7	5.887504507620639E-5
8	0.0
9	0.0012363759466003342
10	0.0010891883339098182
11	2.9437522538103197E-5
12	1.7662513522861915E-4
13	4.415628380715479E-4
14	2.3550018030482557E-4
15	0.004739441128634614
16	6.476254958382703E-4
17	0.0
18	2.943752253810319E-4
19	5.887504507620639E-5
20	4.121253155334447E-4
21	0.0
22	1.4718761269051596E-4
23	8.831256761430958E-4
24	4.415628380715479E-4
25	0.0020017515325910173
26	0.005652004327315813
27	0.006417379913306496
28	0.003974065542643931
29	0.002266689235433946
30	0.0038857529750296214
31	0.007359380634525798
32	0.003208689956653248
33	0.004680566083558408
34	0.002737689596043597
35	0.002884877208734113
36	0.007948131085287862
37	0.0032675650017294543
38	0.007948131085287862
39	0.004474503425791685
40	0.002619939505891184
41	0.003385315091881867
42	0.00211950162274343
43	0.0015601886945194694
44	0.0013246885142146438
45	0.0015013136494432628
46	0.0011186258564479213
47	0.0020900641002053267
48	0.001972314010052914
49	4.7100036060965115E-4
50	0.0014130010818289532
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3397025.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.58276511643831
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.37394683109288	37.372487016249885
2	13.549806750437563	13.436737709601518
3	4.470174261118558	6.6493080125576896
4	1.9819188443952789	3.930760661659759
5	1.1224494961814717	2.782707486211521
6	0.6892959280086534	2.050631885650226
7	0.4859318753810078	1.6865692227724816
8	0.36041930463211674	1.4296468580003416
9	0.27062461033783025	1.2076484840197454
>10	1.5219010245278684	13.700685985506844
>50	0.09885097624713576	3.380601850747242
>100	0.06237305800033557	6.202890773104555
>500	0.00800555005885402	2.760462000460745
>1k	0.004301489580447967	3.4088620534575864
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.831256761430958E-5
2	0.0	0.0	0.0	0.0	8.831256761430958E-5
3	0.0	0.0	0.0	0.0	8.831256761430958E-5
4	0.0	0.0	0.0	0.0	8.831256761430958E-5
5	0.0	2.9437522538103193E-5	0.0	0.0	8.831256761430958E-5
6	0.0	2.9437522538103193E-5	0.0	0.0	8.831256761430958E-5
7	0.0	2.9437522538103193E-5	0.0	0.0	8.831256761430958E-5
8	0.0	2.9437522538103193E-5	0.0	0.0	8.831256761430958E-5
9	0.0	2.9437522538103193E-5	0.0	2.9437522538103193E-5	1.1775009015241277E-4
10	0.0	2.9437522538103193E-5	0.0	2.9437522538103193E-5	1.4718761269051596E-4
11	0.0	2.9437522538103193E-5	0.0	2.9437522538103193E-5	1.4718761269051596E-4
12	0.0	2.9437522538103193E-5	0.0	2.9437522538103193E-5	4.415628380715479E-4
13	0.0	2.9437522538103193E-5	0.0	2.9437522538103193E-5	4.710003606096511E-4
14	0.0	2.9437522538103193E-5	0.0	2.9437522538103193E-5	5.004378831477543E-4
15	0.0	2.9437522538103193E-5	0.0	5.8875045076206386E-5	6.476254958382703E-4
16	0.0	2.9437522538103193E-5	0.0	8.831256761430958E-5	7.065005409144766E-4
17	0.0	2.9437522538103193E-5	0.0	1.1775009015241277E-4	7.065005409144766E-4
18	0.0	2.9437522538103193E-5	0.0	1.1775009015241277E-4	7.653755859906831E-4
19	0.0	2.9437522538103193E-5	0.0	1.1775009015241277E-4	7.653755859906831E-4
20	0.0	2.9437522538103193E-5	0.0	1.7662513522861915E-4	8.536881536049926E-4
21	0.0	2.9437522538103193E-5	0.0	1.7662513522861915E-4	8.536881536049926E-4
22	0.0	2.9437522538103193E-5	0.0	3.8268779299534153E-4	8.536881536049926E-4
23	0.0	2.9437522538103193E-5	0.0	5.887504507620638E-4	8.831256761430958E-4
24	0.0	2.9437522538103193E-5	0.0	9.12563198681199E-4	8.831256761430958E-4
25	0.0	2.9437522538103193E-5	0.0	0.0010008757662955086	8.831256761430958E-4
26	0.0	2.9437522538103193E-5	0.0	0.0011480633789860246	9.12563198681199E-4
27	0.0	2.9437522538103193E-5	0.0	0.0017956888748242947	9.12563198681199E-4
28	0.0	2.9437522538103193E-5	0.0	0.00350306518203428	9.12563198681199E-4
29	0.0	2.9437522538103193E-5	0.0	0.007359380634525799	9.12563198681199E-4
30	0.0	2.9437522538103193E-5	0.0	0.015307511719813661	9.12563198681199E-4
31	0.0	2.9437522538103193E-5	0.0	0.03244014983698972	9.420007212193022E-4
32	0.0	2.9437522538103193E-5	0.0	0.0525754152530523	9.714382437574054E-4
33	0.0	2.9437522538103193E-5	0.0	0.07318168102972454	9.714382437574054E-4
34	0.0	2.9437522538103193E-5	0.0	0.09720269942081675	0.0010891883339098182
35	0.0	2.9437522538103193E-5	0.0	0.12678740957161044	0.0010891883339098182
36	0.0	2.9437522538103193E-5	0.0	0.16805881617003113	0.0010891883339098182
37	0.0	2.9437522538103193E-5	0.0	0.22911223791405716	0.0010891883339098182
38	0.0	2.9437522538103193E-5	0.0	0.31189055129120336	0.0010891883339098182
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4415	0.0	26.559776	1
GGTATCA	1865	0.0	22.17721	1
GTATCGC	115	2.0260268E-7	19.128695	6
AACGCAG	6240	0.0	18.648943	6
TCAACGC	6420	0.0	18.126076	4
CAACGCA	6525	0.0	17.868107	5
ATCAACG	6570	0.0	17.745981	3
TATCAAC	6665	0.0	17.658327	2
CGACGGT	850	0.0	17.3396	7
ACGACGG	945	0.0	16.062033	6
GACGGTA	960	0.0	16.040443	8
CTAGATA	755	0.0	16.025269	3
TAGGCCG	110	5.6304307E-5	15.998545	5
TACGACG	950	0.0	15.977493	5
ACGGTAT	955	0.0	15.894776	9
TATATCG	85	0.0029832353	15.527998	5
ACGCAGA	7515	0.0	15.484951	7
TCTAGAT	915	0.0	15.387031	2
GTATTAG	870	0.0	15.172597	1
AGAGTAC	7730	0.0	15.112286	11
>>END_MODULE