FastQCFastQC Report
Thu 2 Feb 2017
SRR4064159_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064159_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20214
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCAGAGTACTTTTTTTTTTTTTT7063.4926288710794497No Hit
GTATCAACGCAGAGTACTTTTTTTT1210.5985950331453448No Hit
TATCAACGCAGAGTACTTTTTTTTT700.3462946472741664No Hit
ACGCAGAGTACATGGGGAATAATTG630.3116651825467498No Hit
ACGCAGAGTACATGGGAATAACGCC630.3116651825467498No Hit
GGTATCAACGCAGAGTACTTTTTTT460.22756505392302365No Hit
ACGCAGAGTACATGGGTACCTGGTT320.15830612446819037No Hit
GTACTTTTTTTTTTTTTTTTTTTTT320.15830612446819037No Hit
CCGCAGCTAGGAATAATGGAATAGG290.14346492529929752No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGA250.00583284319.012
CATGGGG601.3003373E-615.83333411
GAGTACA1350.015.4814816
CAGAGTA2550.015.2745094
AGAGTAC2550.015.2745095
GCAGAGT2500.015.23
CGCAGAG2500.015.22
CATGGGA508.056165E-515.211
ACGCAGA2550.014.901961
GTACTTT1250.014.4400018
AGTACTT1250.014.4400017
TACTTTT1250.014.4400019
AGTACAT1450.014.4137937
ACTTTTT1300.013.88461510
GAGTACT1300.013.8846156
GTACATG1550.013.4838718
ACATGGG1500.013.310
TACATGG1500.013.39
CTTTTTT1401.8189894E-1212.89285811
Produced by FastQC (version 0.11.5)