##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4064159_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 20214 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.37439398436727 32.0 32.0 32.0 14.0 32.0 2 28.035965172652617 32.0 32.0 32.0 14.0 32.0 3 28.24487978628673 32.0 32.0 32.0 14.0 32.0 4 27.97021866033442 32.0 21.0 32.0 14.0 32.0 5 28.06421292173741 32.0 21.0 32.0 14.0 32.0 6 31.62906896210547 36.0 32.0 36.0 21.0 36.0 7 31.27332541802711 36.0 32.0 36.0 14.0 36.0 8 31.2989512219254 36.0 32.0 36.0 14.0 36.0 9 31.541456416345106 36.0 32.0 36.0 14.0 36.0 10 30.44622538834471 36.0 21.0 36.0 14.0 36.0 11 31.864697734243595 36.0 32.0 36.0 14.0 36.0 12 31.07920253289799 36.0 32.0 36.0 14.0 36.0 13 31.325764321757198 36.0 32.0 36.0 14.0 36.0 14 31.004303947758977 36.0 32.0 36.0 14.0 36.0 15 30.916542990006924 36.0 32.0 36.0 14.0 36.0 16 30.903334322746613 36.0 32.0 36.0 14.0 36.0 17 30.7023844859998 36.0 27.0 36.0 14.0 36.0 18 30.77040664885723 36.0 32.0 36.0 14.0 36.0 19 30.80686652814881 36.0 32.0 36.0 14.0 36.0 20 30.708370436331254 36.0 27.0 36.0 14.0 36.0 21 30.71742356782428 36.0 27.0 36.0 14.0 36.0 22 30.59048184426635 36.0 27.0 36.0 14.0 36.0 23 30.554516671613733 36.0 27.0 36.0 14.0 36.0 24 30.39032353814188 36.0 27.0 36.0 14.0 36.0 25 29.612149995052935 36.0 21.0 36.0 14.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 0.0 9 3.0 10 2.0 11 1.0 12 0.0 13 2.0 14 19.0 15 43.0 16 99.0 17 154.0 18 222.0 19 379.0 20 449.0 21 519.0 22 509.0 23 558.0 24 623.0 25 624.0 26 645.0 27 773.0 28 809.0 29 947.0 30 1014.0 31 1277.0 32 1657.0 33 1843.0 34 3045.0 35 3996.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.06314018506606 26.32985303577614 10.648720866940472 26.958285912217328 2 12.887745510315144 18.423786671943798 33.59224261613813 35.09622520160293 3 29.212427030770755 21.27733254180271 24.567131690907292 24.943108736519243 4 10.69608667689111 14.149309850096472 31.17795478157621 43.9766486914362 5 12.634807559117444 43.78153754823389 29.924804590877606 13.65885030177105 6 40.99544824856521 31.976053829408276 14.842667722145261 12.18583019988126 7 25.893299020093043 37.86993962189449 19.469464515490447 16.767296842522022 8 33.415817084034444 30.253390082153814 18.647926358507373 17.682866475304365 9 24.83915668613283 12.337919429872315 26.42284469959418 36.400079184400674 10 13.423748948176014 34.03949908429441 29.119437707271196 23.417314260258376 11 32.98851712532172 19.525836468026135 20.594931696693724 26.890714709958424 12 21.562221562221563 27.78932778932779 30.42273042273042 20.225720225720227 13 25.89347589347589 18.513018513018515 32.38293238293238 23.21057321057321 14 27.126027126027125 17.508167508167507 27.80912780912781 27.556677556677556 15 27.630927630927633 24.745074745074742 24.844074844074846 22.779922779922778 16 27.75330396475771 23.813295055189823 25.53086175320497 22.902539226847498 17 22.590704350838983 25.46651487402861 28.213631638865515 23.72914913626689 18 22.542322542322545 24.957924957924956 30.100980100980102 22.398772398772397 19 23.505246485844385 23.930904771332408 29.919817857849928 22.644030884973272 20 23.58299094104252 23.48893619127766 29.736151675659624 23.191921192020196 21 23.83686398732924 24.089289249653532 29.221936250247477 22.85191051276975 22 23.096723096723096 23.735273735273736 29.333729333729337 23.834273834273834 23 22.7007227007227 23.26997326997327 29.2990792990793 24.73022473022473 24 22.48948280128681 23.890126206384558 29.507547636723586 24.112843355605047 25 22.651222651222653 23.472923472923473 29.635679635679637 24.24017424017424 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2.0 1 2.0 2 9.0 3 16.0 4 16.0 5 16.0 6 30.5 7 45.0 8 45.0 9 45.0 10 49.0 11 53.0 12 53.0 13 53.0 14 64.5 15 76.0 16 76.0 17 76.0 18 125.5 19 175.0 20 175.0 21 175.0 22 611.0 23 1047.0 24 1047.0 25 1047.0 26 847.5 27 648.0 28 648.0 29 648.0 30 829.5 31 1011.0 32 1011.0 33 1011.0 34 1127.0 35 1243.0 36 1243.0 37 1243.0 38 1389.0 39 1535.0 40 1535.0 41 1535.0 42 1799.5 43 2064.0 44 2064.0 45 2064.0 46 2246.5 47 2429.0 48 2429.0 49 2429.0 50 2588.0 51 2747.0 52 2747.0 53 2747.0 54 2509.5 55 2272.0 56 2272.0 57 2272.0 58 2016.0 59 1760.0 60 1760.0 61 1760.0 62 1534.0 63 1308.0 64 1308.0 65 1308.0 66 1088.0 67 868.0 68 868.0 69 868.0 70 653.0 71 438.0 72 438.0 73 438.0 74 334.0 75 230.0 76 230.0 77 230.0 78 177.0 79 124.0 80 124.0 81 124.0 82 95.0 83 66.0 84 66.0 85 66.0 86 47.0 87 28.0 88 28.0 89 28.0 90 19.5 91 11.0 92 11.0 93 11.0 94 6.5 95 2.0 96 2.0 97 2.0 98 9.0 99 16.0 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024735331948154745 2 0.004947066389630949 3 0.0 4 0.004947066389630949 5 0.0 6 0.009894132779261898 7 0.03957653111704759 8 0.03957653111704759 9 0.03957653111704759 10 0.05441773028594044 11 0.04947066389630949 12 0.05936479667557139 13 0.05936479667557139 14 0.05936479667557139 15 0.05936479667557139 16 0.05441773028594044 17 0.05441773028594044 18 0.05936479667557139 19 0.04947066389630949 20 0.06431186306520234 21 0.04947066389630949 22 0.05936479667557139 23 0.05936479667557139 24 0.04452359750667854 25 0.05936479667557139 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 20214.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.63015731671119 #Duplication Level Percentage of deduplicated Percentage of total 1 93.9899438036084 78.60393786484615 2 3.7503697131026326 6.2728801820520435 3 0.8991422656018929 2.2558622736717124 4 0.4909790002957705 1.642426041357475 5 0.2070393374741201 0.865736618185416 6 0.11830819284235432 0.5936479667557139 7 0.13605442176870747 0.7964776887305828 8 0.11239278320023662 0.7519540912239042 9 0.08873114463176575 0.667853962600178 >10 0.1774622892635315 2.488374393984367 >50 0.01774622892635315 0.969625012367666 >100 0.005915409642117717 0.5985950331453448 >500 0.005915409642117717 3.4926288710794497 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACGCAGAGTACTTTTTTTTTTTTTT 706 3.4926288710794497 No Hit GTATCAACGCAGAGTACTTTTTTTT 121 0.5985950331453448 No Hit TATCAACGCAGAGTACTTTTTTTTT 70 0.3462946472741664 No Hit ACGCAGAGTACATGGGGAATAATTG 63 0.3116651825467498 No Hit ACGCAGAGTACATGGGAATAACGCC 63 0.3116651825467498 No Hit GGTATCAACGCAGAGTACTTTTTTT 46 0.22756505392302365 No Hit ACGCAGAGTACATGGGTACCTGGTT 32 0.15830612446819037 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 32 0.15830612446819037 No Hit CCGCAGCTAGGAATAATGGAATAGG 29 0.14346492529929752 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGGA 25 0.005832843 19.0 12 CATGGGG 60 1.3003373E-6 15.833334 11 GAGTACA 135 0.0 15.481481 6 CAGAGTA 255 0.0 15.274509 4 AGAGTAC 255 0.0 15.274509 5 GCAGAGT 250 0.0 15.2 3 CGCAGAG 250 0.0 15.2 2 CATGGGA 50 8.056165E-5 15.2 11 ACGCAGA 255 0.0 14.90196 1 GTACTTT 125 0.0 14.440001 8 AGTACTT 125 0.0 14.440001 7 TACTTTT 125 0.0 14.440001 9 AGTACAT 145 0.0 14.413793 7 ACTTTTT 130 0.0 13.884615 10 GAGTACT 130 0.0 13.884615 6 GTACATG 155 0.0 13.483871 8 ACATGGG 150 0.0 13.3 10 TACATGG 150 0.0 13.3 9 CTTTTTT 140 1.8189894E-12 12.892858 11 >>END_MODULE