##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064159_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20214
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.83936875432868	32.0	32.0	32.0	21.0	32.0
2	29.808103294746214	32.0	32.0	32.0	21.0	32.0
3	30.712822796081923	32.0	32.0	32.0	32.0	32.0
4	31.045562481448503	32.0	32.0	32.0	32.0	32.0
5	30.814979717027803	32.0	32.0	32.0	32.0	32.0
6	34.40744038785	36.0	36.0	36.0	32.0	36.0
7	34.48575244879786	36.0	36.0	36.0	32.0	36.0
8	34.330661917482935	36.0	36.0	36.0	32.0	36.0
9	34.6072523993272	36.0	36.0	36.0	32.0	36.0
10	34.166765607994456	36.0	36.0	36.0	32.0	36.0
11	34.75680221628574	36.0	36.0	36.0	32.0	36.0
12	34.44009102602157	36.0	36.0	36.0	32.0	36.0
13	34.62758484218858	36.0	36.0	36.0	32.0	36.0
14	34.499950529336104	36.0	36.0	36.0	32.0	36.0
15	34.388789947561094	36.0	36.0	36.0	32.0	36.0
16	34.45092510141486	36.0	36.0	36.0	32.0	36.0
17	34.31329771445533	36.0	36.0	36.0	32.0	36.0
18	34.38552488374394	36.0	36.0	36.0	32.0	36.0
19	34.45349757593747	36.0	36.0	36.0	32.0	36.0
20	34.39591372316217	36.0	36.0	36.0	32.0	36.0
21	34.44004155535767	36.0	36.0	36.0	32.0	36.0
22	34.34629464727416	36.0	36.0	36.0	32.0	36.0
23	34.25754427624419	36.0	36.0	36.0	32.0	36.0
24	34.29311368358563	36.0	36.0	36.0	32.0	36.0
25	34.21480162263778	36.0	36.0	36.0	32.0	36.0
26	34.11996635994855	36.0	36.0	36.0	32.0	36.0
27	34.11200158306124	36.0	36.0	36.0	32.0	36.0
28	34.088700900366085	36.0	36.0	36.0	32.0	36.0
29	34.05372514099139	36.0	36.0	36.0	32.0	36.0
30	33.96744830315623	36.0	36.0	36.0	32.0	36.0
31	33.97368160680716	36.0	36.0	36.0	32.0	36.0
32	33.860641139804095	36.0	36.0	36.0	32.0	36.0
33	33.8284357376076	36.0	36.0	36.0	32.0	36.0
34	33.794993568813695	36.0	36.0	36.0	32.0	36.0
35	33.81552389433066	36.0	36.0	36.0	32.0	36.0
36	33.78663302661522	36.0	36.0	36.0	32.0	36.0
37	33.77134659147126	36.0	36.0	36.0	32.0	36.0
38	33.71361432670427	36.0	36.0	36.0	32.0	36.0
39	33.68348669239141	36.0	36.0	36.0	32.0	36.0
40	33.691253586623134	36.0	36.0	36.0	27.0	36.0
41	33.66236271890769	36.0	36.0	36.0	27.0	36.0
42	33.547096072029284	36.0	36.0	36.0	21.0	36.0
43	33.49643811219946	36.0	36.0	36.0	21.0	36.0
44	33.50885524883744	36.0	36.0	36.0	21.0	36.0
45	33.357722370634214	36.0	36.0	36.0	21.0	36.0
46	33.339566636984266	36.0	36.0	36.0	21.0	36.0
47	33.38542594241615	36.0	36.0	36.0	21.0	36.0
48	33.21945186504403	36.0	36.0	36.0	21.0	36.0
49	33.22222222222222	36.0	36.0	36.0	21.0	36.0
50	32.33471851192243	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	3.0
23	9.0
24	32.0
25	60.0
26	143.0
27	242.0
28	373.0
29	614.0
30	933.0
31	1341.0
32	1959.0
33	2982.0
34	4715.0
35	6807.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.331288343558285	18.607757767662775	13.081337819117355	25.97961606966159
2	17.94300979519145	22.04907489858514	35.46057188087464	24.54734342534877
3	20.268130998318	24.052636786385673	29.479568615810827	26.199663599485508
4	14.113980409617097	17.25042050064312	36.51924408825566	32.11635500148412
5	18.165627782724844	34.89660631245671	31.34461264470169	15.593153260116752
6	33.239339071930345	37.16730978529732	16.157118828534678	13.436232314237657
7	29.355891956070053	30.211734441476207	20.634213911150688	19.79815969130306
8	27.78272484416741	32.79410309686356	19.29850598595033	20.124666073018698
9	26.981300089047195	13.955674285148906	18.066686454932228	40.99633917087167
10	14.915405164737312	26.110616404472147	32.90293855743544	26.0710398733551
11	37.77579895122192	20.475907786682498	22.632828732561592	19.115464529533988
12	24.18620757890571	23.46393588601959	28.88592064905511	23.46393588601959
13	28.82655585237954	19.560700504600774	25.49718017215791	26.11556347086178
14	23.57277134659147	19.179776392599187	25.81873948748392	31.42871277332542
15	24.91837340457109	26.85267636291679	22.32116355001484	25.90778668249728
16	25.12120312654596	25.764321757197983	24.072425051944197	25.04205006431186
17	23.57277134659147	26.33818145839517	25.096467794597803	24.992579400415554
18	24.220837043633125	25.754427624418717	25.80389828831503	24.220837043633125
19	24.45334916394578	25.620856831898685	25.695062827743147	24.230731176412387
20	25.170673790442265	25.348768180468984	24.888691006233305	24.591867022855446
21	26.2145047986544	24.76501434649253	24.75512021371327	24.265360641139804
22	24.67596715147917	24.878796873454043	25.541703769664586	24.903532205402197
23	23.854754130800433	24.428613831997627	25.78905708914614	25.927574948055803
24	23.61729494409815	25.50707430493717	25.774215889977242	25.101414860987436
25	24.923320470960718	24.60176115563471	25.615909765509056	24.859008607895518
26	23.70374035226598	25.47991292301603	26.13299030279042	24.68335642192757
27	24.950524440926184	24.84167821096378	25.420542252127447	24.787255095982587
28	24.016624610360697	25.421800009895602	25.505912621839595	25.055662757904113
29	24.558452481076536	25.918963043585812	24.969079305397514	24.553505169940138
30	24.774863928748143	25.22018802572984	25.754576942107864	24.25037110341415
31	25.16451437336104	25.69392904853792	25.00123695017565	24.14031962792539
32	24.884975016078762	25.201602928808192	24.97897392767031	24.934448127442735
33	23.4947805867511	25.656755553356753	25.879384554494635	24.969079305397514
34	24.252919057985356	25.940035622402537	25.41559469622007	24.391450623392043
35	25.566439101612744	25.18056792322153	25.037102997922233	24.215889977243492
36	23.8533471871753	25.763198258374153	25.7038246499431	24.679629904507447
37	24.86518577153317	25.701281353584328	25.458863107900854	23.974669766981645
38	24.713041757371858	25.578864041163662	24.9455768850188	24.762517316445674
39	24.296244990847473	25.354969574036513	24.934448127442735	25.41433730767328
40	24.724160110830738	26.2678739300381	25.263470387412795	23.74449557171837
41	24.776134171078017	25.488546974719238	25.043288972443477	24.692029881759264
42	24.711819126304853	26.18611784495127	25.310443773808935	23.791619254934943
43	24.35440783615316	25.81873948748392	25.309191649351938	24.51766102701098
44	24.695755417037695	25.66538042940536	25.126150192935587	24.51271396062135
45	24.65861864239066	25.65307737977439	25.247377795369086	24.440926182465862
46	24.523821303121753	26.294958689952015	24.83055459357839	24.350665413347848
47	24.646284753141387	25.418027109923813	25.096467794597803	24.839220342336993
48	24.5522904917384	26.921935292371625	24.448402097556148	24.077372118333827
49	24.726661059714043	25.41928461880968	25.374758818582098	24.47929550289418
50	25.140991392104482	25.84347481943208	24.81448501038884	24.2010487780746
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.5
4	3.0
5	4.0
6	5.0
7	5.5
8	6.0
9	8.0
10	10.0
11	12.0
12	14.0
13	21.0
14	28.0
15	44.0
16	60.0
17	58.0
18	56.0
19	65.0
20	74.0
21	82.0
22	90.0
23	122.0
24	154.0
25	171.0
26	188.0
27	237.0
28	286.0
29	299.0
30	312.0
31	361.0
32	410.0
33	453.0
34	496.0
35	578.0
36	660.0
37	696.0
38	732.0
39	825.0
40	918.0
41	966.5
42	1015.0
43	1057.5
44	1100.0
45	1161.0
46	1222.0
47	1329.0
48	1436.0
49	1450.0
50	1464.0
51	1424.5
52	1385.0
53	1394.5
54	1404.0
55	1416.5
56	1429.0
57	1372.5
58	1316.0
59	1140.0
60	964.0
61	869.0
62	774.0
63	687.5
64	601.0
65	534.0
66	467.0
67	405.5
68	344.0
69	304.0
70	264.0
71	212.0
72	160.0
73	138.0
74	116.0
75	100.5
76	85.0
77	74.5
78	64.0
79	52.0
80	40.0
81	30.0
82	20.0
83	16.0
84	12.0
85	11.5
86	11.0
87	7.5
88	4.0
89	3.0
90	2.0
91	3.0
92	4.0
93	3.0
94	2.0
95	2.5
96	3.0
97	1.5
98	0.0
99	2.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009894132779261898
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.009894132779261898
27	0.009894132779261898
28	0.014841199168892847
29	0.004947066389630949
30	0.019788265558523796
31	0.014841199168892847
32	0.004947066389630949
33	0.004947066389630949
34	0.009894132779261898
35	0.0
36	0.014841199168892847
37	0.004947066389630949
38	0.009894132779261898
39	0.004947066389630949
40	0.014841199168892847
41	0.004947066389630949
42	0.004947066389630949
43	0.0
44	0.0
45	0.009894132779261898
46	0.004947066389630949
47	0.0
48	0.0
49	0.004947066389630949
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	20214.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.15464529533986
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.25670248561815	86.83090927080241
2	3.1911429501790947	5.817750074205995
3	0.6729621187452512	1.8403086969427132
4	0.2605014653207424	0.9498367468091422
5	0.13567784652122003	0.6183832987038687
6	0.1573863019646152	0.8607895517957851
7	0.0705524801910344	0.45018304145641636
8	0.05969825246933681	0.43534184228752354
9	0.0379897970259416	0.3116651825467498
>10	0.1573863019646152	1.8848322944493914
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.004947066389630949	0.0
31	0.0	0.0	0.0	0.014841199168892847	0.0
32	0.0	0.0	0.0	0.019788265558523796	0.0
33	0.0	0.0	0.0	0.019788265558523796	0.0
34	0.0	0.0	0.0	0.024735331948154745	0.0
35	0.0	0.0	0.0	0.04452359750667854	0.0
36	0.0	0.0	0.0	0.04452359750667854	0.0
37	0.0	0.0	0.0	0.054417730285940435	0.0
38	0.0	0.0	0.0	0.07420599584446423	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE