FastQCFastQC Report
Thu 2 Feb 2017
SRR4064158_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064158_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1934941
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67550.3491062518185309No Hit
TATCAACGCAGAGTACTTTTTTTTT47920.24765613008355292No Hit
GTACTTTTTTTTTTTTTTTTTTTTT41190.21287470780762824No Hit
GAGTACTTTTTTTTTTTTTTTTTTT26690.1379370223691575No Hit
GGTATCAACGCAGAGTACTTTTTTT24880.1285827319799415No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24700.12765247105725705No Hit
GTACATGGGGTGGTATCAACGCAAA23470.12129568808557988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGACG3100.012.2550964
CATACCG701.0936367E-412.2113285
ACTGTCG550.00306853612.0911078
ATAAGAC1103.8298822E-812.0876683
AAGACGG3250.011.3972395
CGGTCCA3550.011.23932910
GGGTAGG3300.011.2283281
ACACCGT600.005881479511.0820826
ATACCGT3000.011.0820826
TCCAACG1658.0035534E-1110.94155318
CGAACGA1408.338247E-910.85844316
CTAGGAC1855.456968E-1210.7808943
CGTCGTA2400.010.68739910
GTATCAA41750.010.6501011
CGTTTAT1252.2377026E-710.6411
TGAGTTA5200.010.59687412
ACCGTCC909.5153424E-510.5557278
TACCGTC2700.010.5554557
CGCAAGA3250.010.5202562
CCGTCGT2350.010.510819
Produced by FastQC (version 0.11.5)