##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064158_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1934941
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.19539252101227	32.0	32.0	32.0	32.0	32.0
2	30.738967751471492	32.0	32.0	32.0	32.0	32.0
3	30.76203047017971	32.0	32.0	32.0	32.0	32.0
4	30.788777538953383	32.0	32.0	32.0	32.0	32.0
5	30.681523622684104	32.0	32.0	32.0	32.0	32.0
6	34.33784440972619	36.0	36.0	36.0	32.0	36.0
7	34.25452920786732	36.0	36.0	36.0	32.0	36.0
8	34.21474711632034	36.0	36.0	36.0	32.0	36.0
9	34.37242324184562	36.0	36.0	36.0	32.0	36.0
10	34.032570502149674	36.0	36.0	36.0	32.0	36.0
11	34.37888338714204	36.0	36.0	36.0	32.0	36.0
12	34.14394805836457	36.0	36.0	36.0	32.0	36.0
13	34.24669124278208	36.0	36.0	36.0	32.0	36.0
14	34.13965335377151	36.0	36.0	36.0	32.0	36.0
15	34.06448723759536	36.0	36.0	36.0	32.0	36.0
16	34.067494564433744	36.0	36.0	36.0	32.0	36.0
17	33.9784262155797	36.0	36.0	36.0	32.0	36.0
18	33.98895160110825	36.0	36.0	36.0	32.0	36.0
19	34.00567717568649	36.0	36.0	36.0	32.0	36.0
20	33.981553959526416	36.0	36.0	36.0	32.0	36.0
21	33.961829327095764	36.0	36.0	36.0	32.0	36.0
22	33.94140131404524	36.0	36.0	36.0	32.0	36.0
23	33.87995654647868	36.0	36.0	36.0	32.0	36.0
24	33.875827221605206	36.0	36.0	36.0	32.0	36.0
25	33.464642074357826	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	19.0
5	22.0
6	64.0
7	34.0
8	125.0
9	127.0
10	138.0
11	57.0
12	120.0
13	96.0
14	185.0
15	336.0
16	582.0
17	739.0
18	962.0
19	1288.0
20	2164.0
21	3270.0
22	4958.0
23	7602.0
24	11451.0
25	16709.0
26	23700.0
27	31116.0
28	42528.0
29	56794.0
30	74799.0
31	100860.0
32	141253.0
33	199227.0
34	413764.0
35	799848.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.30144401532424	17.480857619985628	11.605788469591923	25.611909895098208
2	16.220408222332942	20.32868037882437	38.02157090966226	25.429340489180436
3	18.451969342243306	24.857021773622545	29.08197280493666	27.60903607919748
4	12.309555274515875	15.979707258179968	36.803490037361506	34.90724742994264
5	14.107429157393442	37.25892632865098	33.926354493684556	14.707290020271019
6	32.81149694062043	36.34898532386833	17.618542558921096	13.220975176590139
7	29.204490413906075	31.151897549832746	21.546780831017085	18.096831205244087
8	27.775405371312058	33.01157642446063	19.990994046351194	19.222024157876124
9	27.560504083931946	14.100410383843586	18.85599234350012	39.48309318872435
10	15.498393114307357	27.205363352664225	32.4494729466638	24.84677058636461
11	36.35580494907794	21.400449652935773	23.237170206252948	19.006575191733337
12	24.471027248638396	23.904303973423634	29.76199587536742	21.86267290257055
13	29.38380586691489	20.04292233900891	25.58031778044965	24.99295401362655
14	23.3607350046698	20.011925312173233	26.221310212225045	30.406029470931923
15	24.659077804880443	27.575823547328344	23.165954682984115	24.599143964807098
16	24.888504135069276	26.1044606620248	24.672286968429503	24.334748234476425
17	23.34681116334582	26.11142971352156	25.94238369877231	24.59937542436031
18	23.878591532687253	25.61026446258765	27.06528968619699	23.445854318528102
19	24.983993173178867	25.166300328411474	26.257712497737323	23.59199400067234
20	25.338913666039637	24.74231389681798	25.75361127762345	24.165161159518934
21	26.44729315554232	24.53294688508854	25.141271865719578	23.878488093649565
22	25.187769655430657	24.75601064605588	25.782498554424492	24.273721144088967
23	24.127643705716263	24.668655895041567	26.054133286576644	25.149567112665526
24	24.167431266207874	25.444407320708734	26.102031886645488	24.286129526437904
25	24.403042816239388	25.0647022558382	26.08866329587395	24.44359163204846
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	624.0
1	624.0
2	969.0
3	1314.0
4	1314.0
5	1314.0
6	3393.5
7	5473.0
8	5473.0
9	5473.0
10	5561.5
11	5650.0
12	5650.0
13	5650.0
14	6151.0
15	6652.0
16	6652.0
17	6652.0
18	11177.5
19	15703.0
20	15703.0
21	15703.0
22	24847.0
23	33991.0
24	33991.0
25	33991.0
26	49476.0
27	64961.0
28	64961.0
29	64961.0
30	81526.0
31	98091.0
32	98091.0
33	98091.0
34	115977.0
35	133863.0
36	133863.0
37	133863.0
38	150087.5
39	166312.0
40	166312.0
41	166312.0
42	187730.5
43	209149.0
44	209149.0
45	209149.0
46	230522.0
47	251895.0
48	251895.0
49	251895.0
50	253944.5
51	255994.0
52	255994.0
53	255994.0
54	235131.5
55	214269.0
56	214269.0
57	214269.0
58	194656.0
59	175043.0
60	175043.0
61	175043.0
62	152462.5
63	129882.0
64	129882.0
65	129882.0
66	105735.5
67	81589.0
68	81589.0
69	81589.0
70	61140.5
71	40692.0
72	40692.0
73	40692.0
74	30915.0
75	21138.0
76	21138.0
77	21138.0
78	16825.0
79	12512.0
80	12512.0
81	12512.0
82	8651.5
83	4791.0
84	4791.0
85	4791.0
86	3693.5
87	2596.0
88	2596.0
89	2596.0
90	1896.5
91	1197.0
92	1197.0
93	1197.0
94	837.5
95	478.0
96	478.0
97	478.0
98	780.0
99	1082.0
100	1082.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.038812552940890706
2	0.005839971347963581
3	0.001602116033512133
4	0.003204232067024266
5	0.0082173048170461
6	0.012145073157269395
7	0.02170608819597083
8	0.03436797297695382
9	0.0464096838094805
10	0.05560893071158242
11	0.05193956818321592
12	0.061655626709031436
13	0.06284429344357269
14	0.06403296017811395
15	0.059019887428092124
16	0.06392959785337124
17	0.05932997440232028
18	0.07416246800290034
19	0.07188849685856054
20	0.07669484495909694
21	0.07416246800290034
22	0.07519609125032753
23	0.07896881610343674
24	0.07581626519878384
25	0.07591962752352656
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1934941.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.36736851940259
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.01542359862678	36.27227379472917
2	15.64187611126934	15.756802768622855
3	5.586848800996309	8.441846172658908
4	2.533551483922551	5.104332850144256
5	1.324224730188323	3.3348857493950868
6	0.7250726544355397	2.191200095357776
7	0.4839092270112119	1.7061264056769068
8	0.3159760952035091	1.2731907544349585
9	0.2339328901594136	1.0604325678721302
>10	0.9920726928231713	8.642647139776193
>50	0.06450967636387683	2.2603012498950563
>100	0.06940365495383408	7.471773069099006
>500	0.009589450908532379	3.264010256985548
>1k	0.0035058207622591492	2.869356267314704
>5k	1.031123753605632E-4	0.3508208580374455
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6755	0.3491062518185309	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4792	0.24765613008355292	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4119	0.21287470780762824	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2669	0.1379370223691575	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2488	0.1285827319799415	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2470	0.12765247105725705	No Hit
GTACATGGGGTGGTATCAACGCAAA	2347	0.12129568808557988	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.0336232474271825E-4	0.0
5	0.0	0.0	0.0	1.5504348711407737E-4	0.0
6	0.0	0.0	0.0	1.5504348711407737E-4	0.0
7	5.1681162371359124E-5	0.0	0.0	1.5504348711407737E-4	0.0
8	5.1681162371359124E-5	0.0	0.0	1.5504348711407737E-4	0.0
9	5.1681162371359124E-5	0.0	0.0	1.5504348711407737E-4	0.0
10	5.1681162371359124E-5	0.0	0.0	1.5504348711407737E-4	0.0
11	5.1681162371359124E-5	0.0	0.0	1.5504348711407737E-4	0.0
12	5.1681162371359124E-5	0.0	0.0	2.067246494854365E-4	0.0
13	5.1681162371359124E-5	0.0	0.0	2.067246494854365E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAGACG	310	0.0	12.255096	4
CATACCG	70	1.0936367E-4	12.211328	5
ACTGTCG	55	0.003068536	12.091107	8
ATAAGAC	110	3.8298822E-8	12.087668	3
AAGACGG	325	0.0	11.397239	5
CGGTCCA	355	0.0	11.239329	10
GGGTAGG	330	0.0	11.228328	1
ACACCGT	60	0.0058814795	11.082082	6
ATACCGT	300	0.0	11.082082	6
TCCAACG	165	8.0035534E-11	10.941553	18
CGAACGA	140	8.338247E-9	10.858443	16
CTAGGAC	185	5.456968E-12	10.780894	3
CGTCGTA	240	0.0	10.687399	10
GTATCAA	4175	0.0	10.650101	1
CGTTTAT	125	2.2377026E-7	10.641	1
TGAGTTA	520	0.0	10.596874	12
ACCGTCC	90	9.5153424E-5	10.555727	8
TACCGTC	270	0.0	10.555455	7
CGCAAGA	325	0.0	10.520256	2
CCGTCGT	235	0.0	10.51081	9
>>END_MODULE