FastQCFastQC Report
Thu 2 Feb 2017
SRR4064158_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064158_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1934941
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21550.11137290491027893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50650.018.679931
TACGTAT600.007411838518.33411825
CGTCGTA5150.017.08634410
ATACCGT5150.017.0859036
TACCGTC5350.016.4471787
AACGCAG57000.016.4406856
CCGTCGT5500.016.3990089
CGACGGT5100.016.3907417
CGTCTTA1351.0869571E-616.2953115
ACGCTAT4750.016.20954710
TACGCTA4900.016.1622879
GATATAC5750.016.0719171
GTATAAT2500.015.8423171
CGCCCCT3350.015.7598335
ACCGTCG5600.015.7129298
GACGGTA5350.015.624828
ACGACGG5350.015.624826
AACGTCT3250.015.5678866
AATAGCG3850.015.427245
TACGACG5300.015.3571645
Produced by FastQC (version 0.11.5)