##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064158_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1934941
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28482573887266	32.0	32.0	32.0	32.0	32.0
2	31.419590571495462	32.0	32.0	32.0	32.0	32.0
3	31.485207042488636	32.0	32.0	32.0	32.0	32.0
4	31.56602656101659	32.0	32.0	32.0	32.0	32.0
5	31.51632995528029	32.0	32.0	32.0	32.0	32.0
6	35.099657302212314	36.0	36.0	36.0	36.0	36.0
7	35.113628270836166	36.0	36.0	36.0	36.0	36.0
8	35.067667179516064	36.0	36.0	36.0	36.0	36.0
9	35.16785162958457	36.0	36.0	36.0	36.0	36.0
10	35.02736362504076	36.0	36.0	36.0	36.0	36.0
11	35.17074008974951	36.0	36.0	36.0	36.0	36.0
12	35.0771439542601	36.0	36.0	36.0	36.0	36.0
13	35.11614876112502	36.0	36.0	36.0	36.0	36.0
14	35.058634345956804	36.0	36.0	36.0	36.0	36.0
15	35.036336508451676	36.0	36.0	36.0	36.0	36.0
16	35.044608595300836	36.0	36.0	36.0	36.0	36.0
17	35.009878854187285	36.0	36.0	36.0	36.0	36.0
18	35.00675007661732	36.0	36.0	36.0	36.0	36.0
19	35.003652824556404	36.0	36.0	36.0	36.0	36.0
20	34.99464841563645	36.0	36.0	36.0	36.0	36.0
21	34.99641797863604	36.0	36.0	36.0	36.0	36.0
22	34.97834197528503	36.0	36.0	36.0	36.0	36.0
23	34.92815904981082	36.0	36.0	36.0	32.0	36.0
24	34.90175721120179	36.0	36.0	36.0	32.0	36.0
25	34.87376566003821	36.0	36.0	36.0	32.0	36.0
26	34.819044094884546	36.0	36.0	36.0	32.0	36.0
27	34.819336093451945	36.0	36.0	36.0	32.0	36.0
28	34.79256835221332	36.0	36.0	36.0	32.0	36.0
29	34.764323563354125	36.0	36.0	36.0	32.0	36.0
30	34.739154320467655	36.0	36.0	36.0	32.0	36.0
31	34.73121454349254	36.0	36.0	36.0	32.0	36.0
32	34.68744421664537	36.0	36.0	36.0	32.0	36.0
33	34.65299355380862	36.0	36.0	36.0	32.0	36.0
34	34.63288699758804	36.0	36.0	36.0	32.0	36.0
35	34.598146920242016	36.0	36.0	36.0	32.0	36.0
36	34.572146644264606	36.0	36.0	36.0	32.0	36.0
37	34.54972838965116	36.0	36.0	36.0	32.0	36.0
38	34.51146107297328	36.0	36.0	36.0	32.0	36.0
39	34.50734053389741	36.0	36.0	36.0	32.0	36.0
40	34.481174361388796	36.0	36.0	36.0	32.0	36.0
41	34.459110639549216	36.0	36.0	36.0	32.0	36.0
42	34.39014626285763	36.0	36.0	36.0	32.0	36.0
43	34.371917283266	36.0	36.0	36.0	32.0	36.0
44	34.30277564018748	36.0	36.0	36.0	32.0	36.0
45	34.26334808141437	36.0	36.0	36.0	32.0	36.0
46	34.2338768985721	36.0	36.0	36.0	32.0	36.0
47	34.18039516450372	36.0	36.0	36.0	32.0	36.0
48	34.1273527203155	36.0	36.0	36.0	32.0	36.0
49	34.104216614356716	36.0	36.0	36.0	32.0	36.0
50	33.51082642829937	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	4.0
20	30.0
21	59.0
22	233.0
23	630.0
24	1578.0
25	3747.0
26	7629.0
27	14027.0
28	23335.0
29	35300.0
30	50340.0
31	70430.0
32	101281.0
33	159989.0
34	326671.0
35	1139656.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.483238463538314	18.413061691985092	12.169331859246407	25.93436798523019
2	15.432265613331314	21.135873955325586	37.61473911499778	25.81712131634532
3	17.699415070488193	25.39109861111542	29.527725493479362	27.381760824917023
4	11.99586964150328	16.670430777992713	36.80949444970157	34.524205130802436
5	13.612714806291251	37.71350134190138	33.77441482711876	14.899369024688609
6	32.84322288362728	36.76520901444904	17.27559229287967	13.115975809044011
7	28.952680703277622	31.390068942706232	21.568110639089586	18.08913971492656
8	27.63438265042707	33.32649419284619	19.920452354878005	19.11867080184874
9	27.517375467204708	13.899598534713723	18.819202113584492	39.76382388449707
10	15.343949798415334	27.474948563738327	32.45040231658276	24.73069932126358
11	36.537050244452026	21.29053097253662	23.295192615791706	18.877226167219654
12	24.31980989561934	24.11820126443393	29.740399816634856	21.821589023311873
13	29.416826448502015	20.080519708723312	25.587127182895504	24.91552665987917
14	23.343252696730023	19.965208151587444	26.23399430265394	30.4575448490286
15	24.63817552498983	27.66156703863406	23.179869002561958	24.520388433814148
16	24.9164825078401	26.128881547802397	24.661020277799025	24.29361566655848
17	23.38856843697043	26.150513116420605	25.94471872785785	24.516199718751114
18	23.87350279285723	25.545289353239593	27.172629999131754	23.408577854771423
19	25.086255904575854	25.070699866662533	26.294148655772272	23.54889557298934
20	25.42393028374684	24.658475840715603	25.85851987333902	24.05907400219853
21	26.49868910731645	24.47873087603188	25.285835588785393	23.736744427866277
22	25.182240887180424	24.743880814847394	25.89952448113351	24.17435381683867
23	24.08846454792493	24.59055197057039	26.19588799961962	25.125095481885058
24	24.287376216260355	25.297750251043063	26.186000815534644	24.228872717161938
25	24.49770221633277	24.761487367874853	26.3318979507036	24.40891246508878
26	24.161227835514033	25.626042715411824	26.487865738568118	23.72486371050602
27	24.571460172646013	25.149083173370627	25.991794610988272	24.28766204299508
28	23.957671684217328	25.350009690548486	26.333690806899668	24.35862781833452
29	24.029576676453548	25.173199896842373	26.277337933736213	24.519885492967862
30	23.922729572626956	25.63034048335105	26.324806821347412	24.122123122674584
31	24.4790422999356	25.280778084785936	25.62959930783615	24.610580307442312
32	24.27292648639709	25.506077896303648	25.54644215661953	24.674553460679732
33	24.036991038567905	24.99210531892186	26.078599496497656	24.892304146012577
34	24.609836306193674	25.202117119654677	26.122426408075277	24.065620166076368
35	24.9911235309896	25.052884115952782	26.23832556976078	23.71766678329684
36	24.141051721927965	25.781266151473424	25.760799004345653	24.316883122252957
37	24.757892212548363	25.55282440996402	25.686631019359318	24.002652358128298
38	24.170362586966757	25.522188184666973	25.98089406039205	24.326555167974213
39	24.782478139279114	25.1547008754625	25.593439948978297	24.469381036280087
40	24.98306113135621	25.403083054981163	25.902850743113724	23.7110050705489
41	23.89327385037749	25.671715689111974	26.40384806211807	24.03116239839247
42	24.971277748692316	25.911217621895787	25.718443897419576	23.399060731992325
43	24.20358464432638	25.1295143985281	26.193692762491473	24.473208194654042
44	24.04931470034937	25.72064994935191	25.483224112624814	24.74681123767391
45	24.12730242077192	25.846753354143843	25.66457527959812	24.361368945486117
46	23.93393415121468	25.63147378855331	25.54795603741347	24.886636022818536
47	24.13918031939635	25.582614088583387	25.93281306527469	24.34539252674557
48	24.81922866417593	25.783463046984785	25.163431965323323	24.23387632351596
49	24.15573434986997	26.043186958121613	25.330553568696846	24.470525123311575
50	24.26012292006475	26.19198972978672	25.192773226331273	24.355114123817263
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	327.0
1	212.0
2	97.0
3	294.0
4	491.0
5	531.0
6	571.0
7	534.0
8	497.0
9	721.5
10	946.0
11	1416.0
12	1886.0
13	3265.0
14	4644.0
15	6210.0
16	7776.0
17	8240.0
18	8704.0
19	8578.0
20	8452.0
21	9412.5
22	10373.0
23	11616.0
24	12859.0
25	14184.5
26	15510.0
27	19167.0
28	22824.0
29	26465.0
30	30106.0
31	34220.5
32	38335.0
33	43662.5
34	48990.0
35	55867.0
36	62744.0
37	70870.5
38	78997.0
39	84643.0
40	90289.0
41	96986.5
42	103684.0
43	106364.0
44	109044.0
45	118368.5
46	127693.0
47	134490.5
48	141288.0
49	144724.0
50	148160.0
51	142695.0
52	137230.0
53	134533.0
54	131836.0
55	130695.5
56	129555.0
57	124773.0
58	119991.0
59	108672.0
60	97353.0
61	85083.5
62	72814.0
63	63011.0
64	53208.0
65	45194.0
66	37180.0
67	31799.5
68	26419.0
69	23613.0
70	20807.0
71	16228.5
72	11650.0
73	10024.0
74	8398.0
75	6475.5
76	4553.0
77	4005.5
78	3458.0
79	2856.0
80	2254.0
81	1829.5
82	1405.0
83	1103.5
84	802.0
85	587.5
86	373.0
87	265.5
88	158.0
89	118.5
90	79.0
91	58.5
92	38.0
93	33.5
94	29.0
95	36.0
96	43.0
97	28.0
98	13.0
99	10.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00950933387633008
2	4.13449298970873E-4
3	3.6176813659951387E-4
4	0.0
5	0.0
6	1.550434871140774E-4
7	5.1681162371359124E-5
8	0.0
9	0.0014987537087694147
10	0.0011369855721699007
11	5.1681162371359124E-5
12	2.067246494854365E-4
13	5.684927860849504E-4
14	2.584058118567956E-4
15	0.004858029262907758
16	8.785797603131052E-4
17	0.0
18	2.584058118567956E-4
19	5.1681162371359124E-5
20	6.201739484563096E-4
21	0.0
22	1.0336232474271825E-4
23	9.819420850558232E-4
24	7.235362731990277E-4
25	0.001705478358254851
26	0.004599623451050962
27	0.005943333672706299
28	0.003410956716509702
29	0.0019638841701116465
30	0.0038244060154805754
31	0.007183681569618919
32	0.0031525509046529065
33	0.004858029262907758
34	0.0024806957938252384
35	0.002584058118567956
36	0.006873594595390764
37	0.0030491885799101884
38	0.007752174355703869
39	0.004031130664966012
40	0.0021706088195970834
41	0.0023256523067111605
42	0.0015504348711407737
43	0.0010853044097985417
44	0.0010853044097985417
45	0.0010853044097985417
46	0.00118866673454126
47	0.0021189276572257244
48	0.0019638841701116465
49	2.584058118567956E-4
50	0.001292029059283978
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1934941.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.16452932374839
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.52542315415594	39.824699384742885
2	15.132557918396508	16.392957542286172
3	5.2599197276335286	8.547032289839066
4	2.3089443759574504	5.00251541433805
5	1.2016395056855091	3.2543119121138644
6	0.6794975572518313	2.2082799219109326
7	0.4197736324462956	1.5915788856781627
8	0.277580552247454	1.2028015969527595
9	0.2010896001023637	0.9802731187300786
>10	0.8730942410704943	7.957934106830495
>50	0.05378512416305983	2.0488422445409795
>100	0.05679767648684646	6.484284767200295
>500	0.007494765858460942	2.765043116749007
>1k	0.002402168544378506	1.7394456980873603
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2155	0.11137290491027893	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.0336232474271825E-4	0.0
5	0.0	0.0	0.0	1.5504348711407737E-4	0.0
6	0.0	0.0	0.0	1.5504348711407737E-4	0.0
7	0.0	0.0	0.0	1.5504348711407737E-4	0.0
8	0.0	0.0	0.0	2.584058118567956E-4	0.0
9	0.0	0.0	0.0	2.584058118567956E-4	0.0
10	0.0	0.0	0.0	2.584058118567956E-4	0.0
11	0.0	0.0	0.0	3.1008697422815474E-4	0.0
12	0.0	0.0	0.0	3.1008697422815474E-4	0.0
13	0.0	5.1681162371359124E-5	0.0	3.1008697422815474E-4	0.0
14	0.0	5.1681162371359124E-5	0.0	3.1008697422815474E-4	0.0
15	0.0	5.1681162371359124E-5	0.0	3.1008697422815474E-4	0.0
16	0.0	5.1681162371359124E-5	0.0	4.13449298970873E-4	0.0
17	0.0	5.1681162371359124E-5	0.0	4.13449298970873E-4	0.0
18	0.0	5.1681162371359124E-5	0.0	4.651304613422321E-4	0.0
19	0.0	5.1681162371359124E-5	0.0	5.168116237135912E-4	0.0
20	0.0	1.0336232474271825E-4	0.0	6.201739484563095E-4	0.0
21	0.0	1.0336232474271825E-4	0.0	8.26898597941746E-4	0.0
22	0.0	1.0336232474271825E-4	0.0	0.0010853044097985417	0.0
23	0.0	1.5504348711407737E-4	0.0	0.002118927657225724	0.0
24	0.0	2.067246494854365E-4	0.0	0.00356600020362378	0.0
25	0.0	2.067246494854365E-4	0.0	0.004599623451050962	0.0
26	0.0	2.067246494854365E-4	0.0	0.005788290185592222	0.0
27	0.0	2.067246494854365E-4	0.0	0.00713200040724756	0.0
28	0.0	2.067246494854365E-4	0.0	0.010491275961385903	0.0
29	0.0	2.067246494854365E-4	0.0	0.01829513147946113	0.0
30	0.0	2.067246494854365E-4	0.0	0.03498814692541013	0.0
31	0.0	2.067246494854365E-4	0.0	0.06558339504925473	0.0
32	0.0	2.067246494854365E-4	0.0	0.10057154197466486	0.0
33	0.0	2.067246494854365E-4	0.0	0.13406093519130557	0.0
34	0.0	2.067246494854365E-4	0.0	0.16972093722754336	0.0
35	0.0	2.067246494854365E-4	0.0	0.2109108236375166	0.0
36	0.0	2.067246494854365E-4	0.0	0.27117105896252136	0.0
37	0.0	2.067246494854365E-4	0.0	0.3611479626510576	0.0
38	0.0	2.067246494854365E-4	0.0	0.48058312889126853	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5065	0.0	18.67993	1
TACGTAT	60	0.0074118385	18.334118	25
CGTCGTA	515	0.0	17.086344	10
ATACCGT	515	0.0	17.085903	6
TACCGTC	535	0.0	16.447178	7
AACGCAG	5700	0.0	16.440685	6
CCGTCGT	550	0.0	16.399008	9
CGACGGT	510	0.0	16.390741	7
CGTCTTA	135	1.0869571E-6	16.29531	15
ACGCTAT	475	0.0	16.209547	10
TACGCTA	490	0.0	16.162287	9
GATATAC	575	0.0	16.071917	1
GTATAAT	250	0.0	15.842317	1
CGCCCCT	335	0.0	15.759833	5
ACCGTCG	560	0.0	15.712929	8
GACGGTA	535	0.0	15.62482	8
ACGACGG	535	0.0	15.62482	6
AACGTCT	325	0.0	15.567886	6
AATAGCG	385	0.0	15.42724	5
TACGACG	530	0.0	15.357164	5
>>END_MODULE