FastQCFastQC Report
Thu 2 Feb 2017
SRR4064157_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064157_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1752769
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35030.19985520054268419No Hit
TATCAACGCAGAGTACTTTTTTTTT25720.1467392451600867No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17810.101610651489158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA852.69034E-713.41557716
ATACCGT2050.012.9744786
ACCGTCG2000.012.3506888
AAGGCGT802.8694041E-511.8739656
CGTTATT1157.136805E-811.5579392
CGTCGTA2350.011.32010110
ACGAACG1104.9525624E-711.23014215
GGCTTAT855.3468753E-511.1716681
AACCGCG1201.2759119E-711.0837
ATAGTAC951.3681498E-510.9943313
TTTCGTC2250.010.98026917
GTAGGAC5200.010.955893
GTATCAA22600.010.882491
CCGATAA1053.4629338E-610.8580579
GGTTCTA2900.010.812180513
TAAAACC1502.1445885E-910.7629624
GGCGAGA2650.010.75777319
TTTTCGT2300.010.74218216
ATTAGCC1158.875777E-710.7335973
CGGTTCT2950.010.62922812
Produced by FastQC (version 0.11.5)