##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064157_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1752769
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2141577127391	32.0	32.0	32.0	32.0	32.0
2	30.920718018175812	32.0	32.0	32.0	32.0	32.0
3	30.90536859106933	32.0	32.0	32.0	32.0	32.0
4	30.92765618287407	32.0	32.0	32.0	32.0	32.0
5	30.879127255217316	32.0	32.0	32.0	32.0	32.0
6	34.37707764114952	36.0	36.0	36.0	32.0	36.0
7	34.306994817913825	36.0	36.0	36.0	32.0	36.0
8	34.24479437963588	36.0	36.0	36.0	32.0	36.0
9	34.44686207937269	36.0	36.0	36.0	32.0	36.0
10	34.125753022788516	36.0	36.0	36.0	32.0	36.0
11	34.3756958275734	36.0	36.0	36.0	32.0	36.0
12	34.212320619545416	36.0	36.0	36.0	32.0	36.0
13	34.289161891840855	36.0	36.0	36.0	32.0	36.0
14	34.201089247927136	36.0	36.0	36.0	32.0	36.0
15	34.18444187454251	36.0	36.0	36.0	32.0	36.0
16	34.19183075465164	36.0	36.0	36.0	32.0	36.0
17	34.093832672759504	36.0	36.0	36.0	32.0	36.0
18	34.114695091024544	36.0	36.0	36.0	32.0	36.0
19	34.117390825602236	36.0	36.0	36.0	32.0	36.0
20	34.04365549596096	36.0	36.0	36.0	32.0	36.0
21	33.998483542326454	36.0	36.0	36.0	32.0	36.0
22	34.03546959125818	36.0	36.0	36.0	32.0	36.0
23	33.94767821658188	36.0	36.0	36.0	32.0	36.0
24	33.92321749186573	36.0	36.0	36.0	32.0	36.0
25	33.38104450729104	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	11.0
5	17.0
6	62.0
7	33.0
8	110.0
9	89.0
10	146.0
11	52.0
12	111.0
13	87.0
14	199.0
15	425.0
16	756.0
17	1011.0
18	1372.0
19	1629.0
20	2248.0
21	2985.0
22	3963.0
23	5728.0
24	8046.0
25	11433.0
26	16061.0
27	22475.0
28	31273.0
29	44223.0
30	61772.0
31	90375.0
32	138439.0
33	211721.0
34	452453.0
35	643461.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.91287066778988	17.375470005273648	11.388453223203136	26.323206103733334
2	16.10270934583385	19.76608313924894	38.20864576070621	25.922561754210992
3	19.028063361076438	23.840392343907965	28.761921280184033	28.369623014831568
4	12.23510442917547	15.574030977013429	36.28259863674668	35.90826595706442
5	14.004804235943901	37.14130844107679	34.26629845602584	14.587588866953475
6	33.907521621216034	35.93523991461638	17.085396742253195	13.071841721914387
7	29.632387767563156	30.834802755094472	21.083605818339525	18.449203659002848
8	27.51335886684621	33.930066481871144	19.41271778962189	19.14385686166075
9	27.093637392626114	14.144271004869463	18.541741464742778	40.22035013776165
10	14.925048521520722	27.47202877040758	32.73809795638771	24.86482475168398
11	36.73159977943368	21.06431243699532	22.987931642705387	19.21615614086562
12	24.11492097998068	23.801112970883274	29.901958545602348	22.182007503533693
13	29.29571273619912	19.880458982702518	25.589827025175545	25.234001255922816
14	22.927367297900435	19.764461944145204	25.93453368797059	31.37363706998377
15	24.633111841804816	27.810830250719278	22.677706991825364	24.878350915650547
16	24.96162240326819	26.105671549427317	24.51205219238979	24.4206538549147
17	23.323670416517764	26.31759522387787	25.961201429000397	24.39753293060397
18	23.911220174492527	25.627027215284908	26.85952843282734	23.602224177395218
19	24.93999470172057	25.320751442182527	25.831510146661856	23.907743709435046
20	25.127852011974156	25.000813616688905	25.581479000981478	24.28985537035546
21	25.927300402110408	24.671811563628847	25.080716113933754	24.320171920326985
22	25.069385009868917	24.987339126979276	25.545673635523297	24.39760222762851
23	24.039628201548606	24.819413730551755	25.817826980910468	25.323131086989164
24	24.29366434689966	25.325141352773016	25.84179337459013	24.539400925737194
25	24.260307056358375	25.081160396695346	25.84920095691048	24.8093315900358
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	112.0
1	112.0
2	390.0
3	668.0
4	668.0
5	668.0
6	1636.0
7	2604.0
8	2604.0
9	2604.0
10	2978.5
11	3353.0
12	3353.0
13	3353.0
14	4173.5
15	4994.0
16	4994.0
17	4994.0
18	8539.5
19	12085.0
20	12085.0
21	12085.0
22	19966.5
23	27848.0
24	27848.0
25	27848.0
26	40552.5
27	53257.0
28	53257.0
29	53257.0
30	68662.5
31	84068.0
32	84068.0
33	84068.0
34	101429.5
35	118791.0
36	118791.0
37	118791.0
38	136586.0
39	154381.0
40	154381.0
41	154381.0
42	174749.0
43	195117.0
44	195117.0
45	195117.0
46	213325.5
47	231534.0
48	231534.0
49	231534.0
50	234945.5
51	238357.0
52	238357.0
53	238357.0
54	220746.5
55	203136.0
56	203136.0
57	203136.0
58	183256.5
59	163377.0
60	163377.0
61	163377.0
62	140174.5
63	116972.0
64	116972.0
65	116972.0
66	94472.0
67	71972.0
68	71972.0
69	71972.0
70	53350.0
71	34728.0
72	34728.0
73	34728.0
74	26171.0
75	17614.0
76	17614.0
77	17614.0
78	13991.0
79	10368.0
80	10368.0
81	10368.0
82	7083.5
83	3799.0
84	3799.0
85	3799.0
86	2864.5
87	1930.0
88	1930.0
89	1930.0
90	1330.5
91	731.0
92	731.0
93	731.0
94	471.5
95	212.0
96	212.0
97	212.0
98	486.5
99	761.0
100	761.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03771175779580766
2	0.005419995447203825
3	0.001198104256750319
4	0.002852629182738855
5	0.008500834964561787
6	0.011866937400193636
7	0.02162292920516052
8	0.03206355201398473
9	0.047353644433464986
10	0.05528395356147901
11	0.05220311404412105
12	0.06053279125771851
13	0.06098921192695672
14	0.06389889369335035
15	0.05756605690767009
16	0.0625296316856357
17	0.060190475755789834
18	0.07359783291466246
19	0.07171509765405482
20	0.07587993626085354
21	0.07188625540501915
22	0.074738884587758
23	0.07930309128014017
24	0.07416835875121022
25	0.07411130616755544
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1752769.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.07837181437242
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.59886665510813	42.39461353030315
2	14.479922896035784	16.240210000146778
3	4.607383999818295	7.751237791002022
4	1.9618374334406843	4.400665961273631
5	1.00453794574262	2.816642621150025
6	0.5789019991483105	1.9478328931393476
7	0.3710162009871384	1.4564189127678868
8	0.24177295624370382	1.0846586987915534
9	0.17823167008793983	0.8995447677859243
>10	0.8518131086222713	8.410612822917333
>50	0.0626419487420449	2.4525692271768893
>100	0.055304562618798486	6.449462144095956
>500	0.006133123740260028	2.317839287127056
>1k	0.0016354996640693404	1.377691342322534
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3503	0.19985520054268419	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2572	0.1467392451600867	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1781	0.101610651489158	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.711577509643313E-4
2	0.0	0.0	0.0	0.0	1.711577509643313E-4
3	0.0	0.0	0.0	0.0	1.711577509643313E-4
4	0.0	0.0	0.0	0.0	1.711577509643313E-4
5	0.0	0.0	0.0	0.0	1.711577509643313E-4
6	0.0	0.0	0.0	0.0	1.711577509643313E-4
7	0.0	0.0	0.0	0.0	1.711577509643313E-4
8	0.0	0.0	0.0	0.0	1.711577509643313E-4
9	0.0	0.0	0.0	0.0	1.711577509643313E-4
10	0.0	0.0	0.0	0.0	1.711577509643313E-4
11	0.0	0.0	0.0	0.0	1.711577509643313E-4
12	0.0	0.0	0.0	0.0	2.282103346191084E-4
13	0.0	0.0	0.0	0.0	2.282103346191084E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	85	2.69034E-7	13.415577	16
ATACCGT	205	0.0	12.974478	6
ACCGTCG	200	0.0	12.350688	8
AAGGCGT	80	2.8694041E-5	11.873965	6
CGTTATT	115	7.136805E-8	11.557939	2
CGTCGTA	235	0.0	11.320101	10
ACGAACG	110	4.9525624E-7	11.230142	15
GGCTTAT	85	5.3468753E-5	11.171668	1
AACCGCG	120	1.2759119E-7	11.083	7
ATAGTAC	95	1.3681498E-5	10.994331	3
TTTCGTC	225	0.0	10.980269	17
GTAGGAC	520	0.0	10.95589	3
GTATCAA	2260	0.0	10.88249	1
CCGATAA	105	3.4629338E-6	10.858057	9
GGTTCTA	290	0.0	10.8121805	13
TAAAACC	150	2.1445885E-9	10.762962	4
GGCGAGA	265	0.0	10.757773	19
TTTTCGT	230	0.0	10.742182	16
ATTAGCC	115	8.875777E-7	10.733597	3
CGGTTCT	295	0.0	10.629228	12
>>END_MODULE