FastQCFastQC Report
Thu 2 Feb 2017
SRR4064157_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064157_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1752769
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTCG500.002578575222.0034127
GTATCAA28250.020.48051
TACGAAT4450.020.26875541
ATACGAA4400.019.99910540
CGAATGC4500.019.0652743
CGCAATA4850.018.14507136
GTAGGAC6600.017.6648673
TAAACGC3550.017.35430728
TCTAGCG4950.017.33552628
CTAGCGG4950.017.33552629
GATATAC5250.017.1801831
TAGCGGC5000.017.1626630
CAATACG5150.017.0875938
AACGCAG33500.016.8768566
TAGGACG5500.016.7987694
GTCCTAA4850.016.7827551
CGAGCCG4850.016.78275515
TATACCG800.00198942616.4992625
AATACGA5350.016.44786339
GCGCAAT5400.016.2969635
Produced by FastQC (version 0.11.5)