##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064157_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1752769
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.13697184283839	32.0	32.0	32.0	32.0	32.0
2	31.044946025403235	32.0	32.0	32.0	32.0	32.0
3	31.264665794522838	32.0	32.0	32.0	32.0	32.0
4	31.41230475892716	32.0	32.0	32.0	32.0	32.0
5	31.295219735173315	32.0	32.0	32.0	32.0	32.0
6	34.877597104923694	36.0	36.0	36.0	36.0	36.0
7	34.91350657160185	36.0	36.0	36.0	36.0	36.0
8	34.83044599716221	36.0	36.0	36.0	32.0	36.0
9	35.014547267780294	36.0	36.0	36.0	36.0	36.0
10	34.785761843117946	36.0	36.0	36.0	32.0	36.0
11	35.02002203370781	36.0	36.0	36.0	36.0	36.0
12	34.89441506553345	36.0	36.0	36.0	32.0	36.0
13	34.96633269985948	36.0	36.0	36.0	36.0	36.0
14	34.895893297975945	36.0	36.0	36.0	32.0	36.0
15	34.8352663699552	36.0	36.0	36.0	32.0	36.0
16	34.86643305535413	36.0	36.0	36.0	32.0	36.0
17	34.82609174397767	36.0	36.0	36.0	32.0	36.0
18	34.82647342576232	36.0	36.0	36.0	32.0	36.0
19	34.83313659700736	36.0	36.0	36.0	32.0	36.0
20	34.826476848917345	36.0	36.0	36.0	32.0	36.0
21	34.82351239667064	36.0	36.0	36.0	32.0	36.0
22	34.80632872899966	36.0	36.0	36.0	32.0	36.0
23	34.744812351199734	36.0	36.0	36.0	32.0	36.0
24	34.72086681131398	36.0	36.0	36.0	32.0	36.0
25	34.691291322473184	36.0	36.0	36.0	32.0	36.0
26	34.625840598504425	36.0	36.0	36.0	32.0	36.0
27	34.62105274568412	36.0	36.0	36.0	32.0	36.0
28	34.60182944814748	36.0	36.0	36.0	32.0	36.0
29	34.56945153639755	36.0	36.0	36.0	32.0	36.0
30	34.54865815175873	36.0	36.0	36.0	32.0	36.0
31	34.54909688612704	36.0	36.0	36.0	32.0	36.0
32	34.495521086920185	36.0	36.0	36.0	32.0	36.0
33	34.47245986208108	36.0	36.0	36.0	32.0	36.0
34	34.461169155775806	36.0	36.0	36.0	32.0	36.0
35	34.42236427047717	36.0	36.0	36.0	32.0	36.0
36	34.39104810730906	36.0	36.0	36.0	32.0	36.0
37	34.38951453386042	36.0	36.0	36.0	32.0	36.0
38	34.34382340171466	36.0	36.0	36.0	32.0	36.0
39	34.34606670930396	36.0	36.0	36.0	32.0	36.0
40	34.32474330616299	36.0	36.0	36.0	32.0	36.0
41	34.30197761370722	36.0	36.0	36.0	32.0	36.0
42	34.23802509058524	36.0	36.0	36.0	32.0	36.0
43	34.22921845377229	36.0	36.0	36.0	32.0	36.0
44	34.172835096923784	36.0	36.0	36.0	32.0	36.0
45	34.13312935132924	36.0	36.0	36.0	32.0	36.0
46	34.11130616755545	36.0	36.0	36.0	32.0	36.0
47	34.08846744779261	36.0	36.0	36.0	32.0	36.0
48	34.038034104893455	36.0	36.0	36.0	32.0	36.0
49	34.019256958560995	36.0	36.0	36.0	32.0	36.0
50	33.3398730808224	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	1.0
19	3.0
20	10.0
21	52.0
22	120.0
23	431.0
24	1275.0
25	3002.0
26	6225.0
27	11725.0
28	20217.0
29	33007.0
30	49856.0
31	75368.0
32	113684.0
33	191529.0
34	382205.0
35	864058.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.797143514109834	17.749022476340016	11.831844819509735	25.621989190040413
2	15.759601999132796	20.390641046121548	38.11149273146352	25.738264223282137
3	18.307344811985203	24.64300609153216	29.17534107616498	27.874308020317656
4	12.184891448901709	16.371010669403667	36.7358733523927	34.70822452930192
5	14.112641198012973	37.204560327116695	33.768397318756776	14.914401156113556
6	33.9640693828672	36.40421117010989	16.864762971918115	12.76695647510479
7	29.80193054532571	30.92347023481132	20.940922620151316	18.33367659971166
8	27.659834239423446	34.07254464222039	19.164076954806937	19.10354416354922
9	27.381050186106908	13.985802929786006	18.210064606824908	40.42308227728218
10	14.912654549728341	27.50832547426819	32.6738564988906	24.90516347711286
11	37.15631503998247	21.07797495161938	22.844152791470407	18.921557216927738
12	24.39960610828827	23.581641816420447	29.794849968563973	22.22390210672731
13	29.633726864384975	19.857447926502328	25.25355681287202	25.255268396240677
14	23.059052411475584	19.6364030546023	25.84254021503168	31.46200431889044
15	24.750372138215756	27.81511363266806	22.56524043637592	24.86927379274027
16	25.132862113801284	26.209337632084768	24.33942640080741	24.318373853306532
17	23.632562894804103	26.213166830978203	25.740714116186513	24.413556158031184
18	24.154450736721273	25.660869733894888	26.620609938805295	23.564069590578548
19	25.104220807191364	25.255296048709212	25.7507406851673	23.889742458932123
20	25.461159454025545	24.890800544970322	25.446667990296422	24.201372010707708
21	26.304835377622492	24.62885868017976	24.87270142272028	24.193604519477464
22	25.34337156243332	24.917929717942954	25.39431960683856	24.344379112785163
23	24.26066392621901	24.742990320951876	25.725158393500518	25.2711873593286
24	24.553291003974866	25.24111930705977	25.697769749817862	24.507819939147506
25	24.721806364449638	24.88828893798039	25.72617895678528	24.66372574078469
26	24.257498897409754	25.744127173586218	25.99545498892845	24.002918940075578
27	24.643201515402215	25.31212378827731	25.56961664203758	24.475058054282893
28	24.137604445699647	25.39326355547568	25.86676023632043	24.602371762504244
29	24.35493156069905	25.202242684374752	25.637679927381484	24.805145827544713
30	24.17596273660476	25.555139692088602	25.893757787930934	24.375139783375705
31	24.802328354598227	25.17758781824593	25.2979766685267	24.722107158629143
32	24.517775881034648	25.41250359442966	25.132993897457194	24.936726627078492
33	24.16557311576425	25.12358076111143	25.747874707593997	24.962971415530326
34	24.711092397372543	25.35295080854191	25.76362608352832	24.17233071055723
35	25.143861947302536	25.15823958391576	25.641944358546308	24.055954110235394
36	24.34434811385078	25.679130685335593	25.393676414790217	24.58284478602341
37	24.912706708232726	25.41107723345307	25.29902231513221	24.377193743181994
38	24.397054510722644	25.425955954596652	25.58708375792944	24.589905776751266
39	24.898798938094906	25.20729457824761	25.26018443554769	24.633722048109803
40	25.218971938728934	25.347799098095773	25.47109205265368	23.962136910521608
41	24.398860509393153	25.497785433211256	25.892316654238318	24.211037403157277
42	25.134005008178654	25.839132372129697	25.301800103039085	23.725062516652564
43	24.556824230764622	25.105277601387993	25.70764634028756	24.630251827559825
44	24.276536870631865	25.595606903437456	25.22105263157895	24.906803594351736
45	24.41437607046315	25.750336899927312	25.354843200326798	24.480443829282734
46	24.38548066126868	25.342718194017177	25.30437848594667	24.967422658767468
47	24.66599228973789	25.39502684100052	25.501774661114183	24.43720620814741
48	25.107446117191433	25.539284514123324	24.947582041593186	24.405687327092053
49	24.593113843685654	25.667531776971558	25.00058479098429	24.738769588358494
50	24.701083840298867	25.781843264494803	24.95320208426877	24.56387081093756
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15.0
1	20.0
2	25.0
3	81.5
4	138.0
5	167.0
6	196.0
7	199.0
8	202.0
9	277.5
10	353.0
11	536.0
12	719.0
13	1319.0
14	1919.0
15	2750.5
16	3582.0
17	3902.5
18	4223.0
19	4457.5
20	4692.0
21	5441.0
22	6190.0
23	7380.0
24	8570.0
25	10072.0
26	11574.0
27	14930.0
28	18286.0
29	21753.5
30	25221.0
31	28754.5
32	32288.0
33	37951.0
34	43614.0
35	50193.0
36	56772.0
37	64317.5
38	71863.0
39	77082.0
40	82301.0
41	88535.0
42	94769.0
43	98552.0
44	102335.0
45	110257.0
46	118179.0
47	124615.0
48	131051.0
49	134096.0
50	137141.0
51	134945.5
52	132750.0
53	129771.5
54	126793.0
55	124454.0
56	122115.0
57	116730.0
58	111345.0
59	100091.0
60	88837.0
61	77717.5
62	66598.0
63	57227.5
64	47857.0
65	40433.0
66	33009.0
67	27687.0
68	22365.0
69	19972.0
70	17579.0
71	13693.0
72	9807.0
73	8233.5
74	6660.0
75	5256.5
76	3853.0
77	3333.0
78	2813.0
79	2289.5
80	1766.0
81	1387.0
82	1008.0
83	869.5
84	731.0
85	517.0
86	303.0
87	216.0
88	129.0
89	106.5
90	84.0
91	69.5
92	55.0
93	43.0
94	31.0
95	30.0
96	29.0
97	24.5
98	20.0
99	17.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007188625540501914
2	5.13473252892994E-4
3	4.564206692382168E-4
4	0.0
5	0.0
6	1.141051673095542E-4
7	0.0
8	0.0
9	7.416835875121023E-4
10	9.128413384764336E-4
11	5.70525836547771E-5
12	1.711577509643313E-4
13	3.423155019286626E-4
14	2.282103346191084E-4
15	0.003423155019286626
16	6.846310038573252E-4
17	5.70525836547771E-5
18	1.711577509643313E-4
19	0.0
20	7.416835875121023E-4
21	0.0
22	1.711577509643313E-4
23	7.987361711668794E-4
24	3.423155019286626E-4
25	0.0018827352606076442
26	0.004450101525072614
27	0.005648205781822933
28	0.0036513653539057343
29	0.0027385240154293006
30	0.0032519972683222945
31	0.006561047120299366
32	0.0032519972683222945
33	0.00393662827217962
34	0.0026244188481197466
35	0.0026814714317745236
36	0.00707452037319236
37	0.0032519972683222945
38	0.007017467789537583
39	0.003993680855834397
40	0.0018827352606076442
41	0.0031949446846675175
42	0.002053893011571976
43	0.001255156840405096
44	0.001083999089440765
45	0.0013122094240598733
46	9.698939221312107E-4
47	0.0022821033461910838
48	0.001711577509643313
49	3.423155019286626E-4
50	0.0011410516730955419
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1752769.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.65657685549027
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.06789724213496	45.97606934916452
2	13.770306558023007	16.429787030047226
3	4.367112542581943	7.815809550993458
4	1.7998912280085384	4.295013975008565
5	0.9112571967648293	2.7181242496959834
6	0.5320895432702871	1.9045584439263963
7	0.3350667236490228	1.3992253625759676
8	0.22131110726169018	1.0562130463464539
9	0.15509959553253302	0.8327439847027811
>10	0.7406366498340811	7.726266612260427
>50	0.05047249080681101	2.0912728304116848
>100	0.04328125882778576	5.26148133178485
>500	0.004616162734792599	1.8283233735958118
>1k	9.617005697484581E-4	0.6651108594858869
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.141051673095542E-4
2	0.0	0.0	0.0	0.0	1.141051673095542E-4
3	0.0	0.0	0.0	0.0	1.141051673095542E-4
4	0.0	0.0	0.0	0.0	1.711577509643313E-4
5	0.0	0.0	0.0	0.0	1.711577509643313E-4
6	0.0	0.0	0.0	0.0	3.423155019286626E-4
7	0.0	0.0	0.0	0.0	3.423155019286626E-4
8	0.0	0.0	0.0	0.0	3.423155019286626E-4
9	0.0	0.0	0.0	0.0	3.423155019286626E-4
10	0.0	0.0	0.0	0.0	3.423155019286626E-4
11	0.0	0.0	0.0	5.70525836547771E-5	3.423155019286626E-4
12	0.0	0.0	0.0	5.70525836547771E-5	3.423155019286626E-4
13	0.0	0.0	0.0	5.70525836547771E-5	3.993680855834397E-4
14	0.0	0.0	0.0	5.70525836547771E-5	3.993680855834397E-4
15	0.0	0.0	0.0	5.70525836547771E-5	4.564206692382168E-4
16	0.0	0.0	0.0	5.70525836547771E-5	4.564206692382168E-4
17	0.0	0.0	0.0	5.70525836547771E-5	4.564206692382168E-4
18	0.0	0.0	0.0	5.70525836547771E-5	4.564206692382168E-4
19	0.0	0.0	0.0	1.141051673095542E-4	4.564206692382168E-4
20	0.0	0.0	0.0	1.141051673095542E-4	5.134732528929938E-4
21	0.0	0.0	0.0	1.141051673095542E-4	5.705258365477709E-4
22	0.0	0.0	0.0	1.711577509643313E-4	6.846310038573252E-4
23	0.0	0.0	0.0	2.8526291827388547E-4	6.846310038573252E-4
24	0.0	0.0	0.0	5.705258365477709E-4	6.846310038573252E-4
25	0.0	0.0	0.0	6.846310038573252E-4	6.846310038573252E-4
26	0.0	0.0	0.0	8.557887548216565E-4	7.987361711668794E-4
27	0.0	0.0	0.0	9.128413384764336E-4	7.987361711668794E-4
28	0.0	0.0	0.0	0.0014833671750242045	7.987361711668794E-4
29	0.0	0.0	0.0	0.002110945595226753	7.987361711668794E-4
30	0.0	0.0	0.0	0.003480207602941403	7.987361711668794E-4
31	0.0	0.0	0.0	0.00867199271552612	7.987361711668794E-4
32	0.0	0.0	0.0	0.014491356248313382	7.987361711668794E-4
33	0.0	0.0	0.0	0.021166508535922304	7.987361711668794E-4
34	0.0	0.0	0.0	0.02852629182738855	8.557887548216565E-4
35	0.0	0.0	0.0	0.03862459913428409	8.557887548216565E-4
36	0.0	0.0	0.0	0.05323006054990703	8.557887548216565E-4
37	0.0	0.0	0.0	0.07371193808197202	8.557887548216565E-4
38	0.0	0.0	0.0	0.10360749191707522	8.557887548216565E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTTCG	50	0.0025785752	22.00341	27
GTATCAA	2825	0.0	20.4805	1
TACGAAT	445	0.0	20.268755	41
ATACGAA	440	0.0	19.999105	40
CGAATGC	450	0.0	19.06527	43
CGCAATA	485	0.0	18.145071	36
GTAGGAC	660	0.0	17.664867	3
TAAACGC	355	0.0	17.354307	28
TCTAGCG	495	0.0	17.335526	28
CTAGCGG	495	0.0	17.335526	29
GATATAC	525	0.0	17.180183	1
TAGCGGC	500	0.0	17.16266	30
CAATACG	515	0.0	17.08759	38
AACGCAG	3350	0.0	16.876856	6
TAGGACG	550	0.0	16.798769	4
GTCCTAA	485	0.0	16.782755	1
CGAGCCG	485	0.0	16.782755	15
TATACCG	80	0.001989426	16.499262	5
AATACGA	535	0.0	16.447863	39
GCGCAAT	540	0.0	16.29696	35
>>END_MODULE