FastQCFastQC Report
Thu 2 Feb 2017
SRR4064156_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064156_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2826254
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47350.1675362511649696No Hit
TATCAACGCAGAGTACTTTTTTTTT30300.10720904773597845No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTCGG400.00528783614.24702555
GGTATCA12100.013.4994011
ACGCTTA500.00149763413.30287717
CGTCGTA4100.012.74440210
GTATCAA31550.012.521761
CGTCTTA1103.8058715E-812.093739515
ACCGTCG4350.012.0123918
CCGTCGT4450.011.9555259
GTCGTAG4550.011.90198511
CGCGTAA2600.011.69276610
TCGCGTA2700.011.6115679
ATACCGT4850.011.5553116
ACGGTAT3950.011.5447569
TACCGTC4700.011.5213197
CGAACGA2150.011.49085716
CTCGACG752.0721588E-411.40165713
GCGTAAC2600.011.32776811
GCGTTAT2550.011.1725551
GGTATAT855.345247E-511.1725541
TTATACT2650.011.1102974
Produced by FastQC (version 0.11.5)