##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064156_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2826254
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27602366949326	32.0	32.0	32.0	32.0	32.0
2	30.86070289506888	32.0	32.0	32.0	32.0	32.0
3	30.880399992357376	32.0	32.0	32.0	32.0	32.0
4	30.916492289794196	32.0	32.0	32.0	32.0	32.0
5	30.813362847076025	32.0	32.0	32.0	32.0	32.0
6	34.47498632465447	36.0	36.0	36.0	32.0	36.0
7	34.42053014343367	36.0	36.0	36.0	32.0	36.0
8	34.38531851701935	36.0	36.0	36.0	32.0	36.0
9	34.537093622866166	36.0	36.0	36.0	32.0	36.0
10	34.21415626479432	36.0	36.0	36.0	32.0	36.0
11	34.50325554603373	36.0	36.0	36.0	32.0	36.0
12	34.313962227032675	36.0	36.0	36.0	32.0	36.0
13	34.40034122906151	36.0	36.0	36.0	32.0	36.0
14	34.290982339166966	36.0	36.0	36.0	32.0	36.0
15	34.22686531359177	36.0	36.0	36.0	32.0	36.0
16	34.22632289949877	36.0	36.0	36.0	32.0	36.0
17	34.15146975466465	36.0	36.0	36.0	32.0	36.0
18	34.14400333444906	36.0	36.0	36.0	32.0	36.0
19	34.166359428416555	36.0	36.0	36.0	32.0	36.0
20	34.151196601579336	36.0	36.0	36.0	32.0	36.0
21	34.132271904789874	36.0	36.0	36.0	32.0	36.0
22	34.116374182929064	36.0	36.0	36.0	32.0	36.0
23	34.05588351223917	36.0	36.0	36.0	32.0	36.0
24	34.03870529683461	36.0	36.0	36.0	32.0	36.0
25	33.634009894369015	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	13.0
4	22.0
5	31.0
6	119.0
7	49.0
8	157.0
9	164.0
10	238.0
11	82.0
12	174.0
13	160.0
14	233.0
15	412.0
16	729.0
17	1000.0
18	1325.0
19	1797.0
20	2632.0
21	4054.0
22	6169.0
23	9772.0
24	14668.0
25	21251.0
26	30733.0
27	40998.0
28	55923.0
29	75275.0
30	98851.0
31	135542.0
32	192745.0
33	273489.0
34	586999.0
35	1270448.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.51946397094739	16.990429063931302	11.443427414502233	26.046679550619068
2	17.071890845656014	19.193868560449243	36.94719559532782	26.787044998566923
3	18.859367143832202	22.9352951645724	28.5839339920027	29.6214036995927
4	12.747417068243749	15.298768748569167	35.47052897777152	36.48328520541557
5	15.0118400733759	36.095238634449586	33.490528649014365	15.402392643160145
6	34.911096657392854	35.03243947544799	16.54261082750211	13.513853039657056
7	30.81594669288421	30.267707016130093	20.292724808880553	18.623621482105147
8	28.580292534025382	32.52611101908366	18.941408246508974	19.952188200381986
9	27.37330693002013	14.092862821949678	18.22218005747464	40.311650190555554
10	16.04050361161452	26.47115363850293	31.268548843621325	26.21979390626123
11	37.500159306697256	20.8909492555067	22.14770208939584	19.461189348400207
12	24.741388039378272	23.424270898020254	28.396227016302344	23.438114046299134
13	29.572418803545897	19.198809253212843	25.020092435826353	26.208679507414907
14	23.830988169497598	19.23435611320517	24.844019637486838	32.090636079810395
15	25.489345009827968	26.79038246939735	21.87862884267887	25.84164367809581
16	26.10999050762099	25.662846701088487	23.085104612673053	25.14205817861747
17	24.49885148301724	25.79560646228446	24.591717082455304	25.113824972242995
18	25.205202681577067	24.960563481022138	25.339933056177077	24.49430078122372
19	25.899304265863986	24.70973309005595	24.981065508207426	24.409897135872637
20	26.13855299886087	24.110713224689448	24.419414543946736	25.331319232502942
21	27.12432383051936	23.94949190579499	24.072501018879457	24.853683244806195
22	26.3214251569521	23.885825977033473	24.562676383002078	25.230072483012343
23	24.94740573312035	23.92859431928801	25.033525414689993	26.09047453290165
24	25.055663987807748	24.721078096716536	24.706206038905304	25.517051876570417
25	25.22540067887547	24.239735248480745	24.727929288172227	25.806934784471558
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	129.0
1	129.0
2	345.5
3	562.0
4	562.0
5	562.0
6	1452.0
7	2342.0
8	2342.0
9	2342.0
10	2969.5
11	3597.0
12	3597.0
13	3597.0
14	5062.5
15	6528.0
16	6528.0
17	6528.0
18	11278.5
19	16029.0
20	16029.0
21	16029.0
22	25317.5
23	34606.0
24	34606.0
25	34606.0
26	51361.5
27	68117.0
28	68117.0
29	68117.0
30	86593.0
31	105069.0
32	105069.0
33	105069.0
34	134273.0
35	163477.0
36	163477.0
37	163477.0
38	192835.5
39	222194.0
40	222194.0
41	222194.0
42	259433.0
43	296672.0
44	296672.0
45	296672.0
46	331783.5
47	366895.0
48	366895.0
49	366895.0
50	379165.0
51	391435.0
52	391435.0
53	391435.0
54	369984.5
55	348534.0
56	348534.0
57	348534.0
58	321058.5
59	293583.0
60	293583.0
61	293583.0
62	256479.0
63	219375.0
64	219375.0
65	219375.0
66	179721.0
67	140067.0
68	140067.0
69	140067.0
70	106002.0
71	71937.0
72	71937.0
73	71937.0
74	54796.0
75	37655.0
76	37655.0
77	37655.0
78	29473.5
79	21292.0
80	21292.0
81	21292.0
82	14711.0
83	8130.0
84	8130.0
85	8130.0
86	6104.0
87	4078.0
88	4078.0
89	4078.0
90	2930.5
91	1783.0
92	1783.0
93	1783.0
94	1260.5
95	738.0
96	738.0
97	738.0
98	1084.0
99	1430.0
100	1430.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03658552982145271
2	0.005802733936864839
3	0.0018045087242689439
4	0.003715165020553708
5	0.00912869119336054
6	0.013303829025982804
7	0.023529378463506816
8	0.03573634924532615
9	0.04797870255115075
10	0.05721354131652711
11	0.053569141343983946
12	0.06199018205723902
13	0.06397160340153434
14	0.06581149464980854
15	0.058805754896764414
16	0.06662529270192984
17	0.06163635681718628
18	0.0741263877910478
19	0.07228649654277358
20	0.07660316447141693
21	0.07334797226293178
22	0.07586013146730619
23	0.08056600716000756
24	0.0766385469954222
25	0.07685084213945385
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2826254.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.684174625385594
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.14143738890908	39.09850083472717
2	12.827611040839395	13.003137560408561
3	4.026960269809657	6.123094725735676
4	1.8511789563624144	3.753019099484466
5	1.001132730997395	2.537079308053057
6	0.6282530696625775	1.9105492975027567
7	0.44999886955277596	1.5965474899947305
8	0.3161972129678147	1.2820955806496768
9	0.24958854323419433	1.1385170378799756
>10	1.339904811996281	12.237703185915482
>50	0.0893575972156995	3.111122778448126
>100	0.06338523056119036	6.711183797135735
>500	0.010369874429604816	3.66906958009661
>1k	0.004624403461820262	3.828379723968035
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4735	0.1675362511649696	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3030	0.10720904773597845	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0614757201582023E-4
2	0.0	0.0	0.0	0.0	1.0614757201582023E-4
3	0.0	0.0	0.0	0.0	1.0614757201582023E-4
4	0.0	0.0	0.0	0.0	1.0614757201582023E-4
5	0.0	0.0	0.0	0.0	1.0614757201582023E-4
6	0.0	0.0	0.0	0.0	1.0614757201582023E-4
7	0.0	0.0	0.0	0.0	1.0614757201582023E-4
8	0.0	0.0	0.0	0.0	1.0614757201582023E-4
9	0.0	0.0	0.0	0.0	1.0614757201582023E-4
10	0.0	0.0	0.0	7.076504801054682E-5	1.0614757201582023E-4
11	0.0	0.0	0.0	7.076504801054682E-5	1.0614757201582023E-4
12	0.0	0.0	0.0	1.0614757201582023E-4	3.184427160474607E-4
13	0.0	0.0	0.0	1.4153009602109365E-4	3.8920776405800754E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATTCGG	40	0.005287836	14.2470255	5
GGTATCA	1210	0.0	13.499401	1
ACGCTTA	50	0.001497634	13.302877	17
CGTCGTA	410	0.0	12.744402	10
GTATCAA	3155	0.0	12.52176	1
CGTCTTA	110	3.8058715E-8	12.0937395	15
ACCGTCG	435	0.0	12.012391	8
CCGTCGT	445	0.0	11.955525	9
GTCGTAG	455	0.0	11.901985	11
CGCGTAA	260	0.0	11.692766	10
TCGCGTA	270	0.0	11.611567	9
ATACCGT	485	0.0	11.555311	6
ACGGTAT	395	0.0	11.544756	9
TACCGTC	470	0.0	11.521319	7
CGAACGA	215	0.0	11.490857	16
CTCGACG	75	2.0721588E-4	11.401657	13
GCGTAAC	260	0.0	11.327768	11
GCGTTAT	255	0.0	11.172555	1
GGTATAT	85	5.345247E-5	11.172554	1
TTATACT	265	0.0	11.110297	4
>>END_MODULE