FastQCFastQC Report
Thu 2 Feb 2017
SRR4064156_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064156_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2826254
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36350.027.113811
GGTATCA15550.024.0511281
CGTCGTA7700.020.85649910
ACCGTCG8550.019.554268
TACCGTC8800.018.7484257
CCGTCGT8950.018.189069
TCAACGC53800.018.1546084
AACGCAG54450.017.9378876
ATCAACG55050.017.782343
CAACGCA55200.017.6941665
AGTATCG1002.486714E-517.5985225
ATACCGT9450.017.458856
TATCAAC56350.017.4111372
GTCGTAG9750.017.14818811
CTCGCGT902.2163175E-417.11148824
CGCAATA7500.016.72155436
ATACGAA7800.016.07699440
TACGAAT8300.015.63834641
GTATTAT3400.015.5292061
ACGCGAG1004.943777E-415.40115837
Produced by FastQC (version 0.11.5)