##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064156_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2826254
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.366427079802452	32.0	32.0	32.0	32.0	32.0
2	31.434752502782835	32.0	32.0	32.0	32.0	32.0
3	31.499434941091636	32.0	32.0	32.0	32.0	32.0
4	31.578543188262625	32.0	32.0	32.0	32.0	32.0
5	31.536623742947377	32.0	32.0	32.0	32.0	32.0
6	35.131162662662305	36.0	36.0	36.0	36.0	36.0
7	35.15350566509592	36.0	36.0	36.0	36.0	36.0
8	35.0950041999056	36.0	36.0	36.0	36.0	36.0
9	35.20437618133402	36.0	36.0	36.0	36.0	36.0
10	35.06556735523417	36.0	36.0	36.0	36.0	36.0
11	35.20103748636888	36.0	36.0	36.0	36.0	36.0
12	35.10630679337385	36.0	36.0	36.0	36.0	36.0
13	35.1490832034205	36.0	36.0	36.0	36.0	36.0
14	35.08914945365845	36.0	36.0	36.0	36.0	36.0
15	35.06925952161412	36.0	36.0	36.0	36.0	36.0
16	35.07903040561818	36.0	36.0	36.0	36.0	36.0
17	35.04246610531113	36.0	36.0	36.0	36.0	36.0
18	35.04458657997476	36.0	36.0	36.0	36.0	36.0
19	35.03779985804531	36.0	36.0	36.0	36.0	36.0
20	35.037317240417885	36.0	36.0	36.0	36.0	36.0
21	35.029432598768544	36.0	36.0	36.0	36.0	36.0
22	35.01551912885395	36.0	36.0	36.0	36.0	36.0
23	34.963185191422994	36.0	36.0	36.0	36.0	36.0
24	34.93490004790794	36.0	36.0	36.0	36.0	36.0
25	34.90606753674652	36.0	36.0	36.0	32.0	36.0
26	34.857649737072464	36.0	36.0	36.0	32.0	36.0
27	34.84722356872383	36.0	36.0	36.0	32.0	36.0
28	34.83488108287507	36.0	36.0	36.0	32.0	36.0
29	34.80294729348459	36.0	36.0	36.0	32.0	36.0
30	34.78871113495107	36.0	36.0	36.0	32.0	36.0
31	34.786755189024056	36.0	36.0	36.0	32.0	36.0
32	34.74360195509674	36.0	36.0	36.0	32.0	36.0
33	34.727237183919065	36.0	36.0	36.0	32.0	36.0
34	34.722309459800854	36.0	36.0	36.0	32.0	36.0
35	34.68958840925126	36.0	36.0	36.0	32.0	36.0
36	34.665691052538094	36.0	36.0	36.0	32.0	36.0
37	34.66158031089916	36.0	36.0	36.0	32.0	36.0
38	34.62627668992242	36.0	36.0	36.0	32.0	36.0
39	34.627025030305134	36.0	36.0	36.0	32.0	36.0
40	34.6103011265088	36.0	36.0	36.0	32.0	36.0
41	34.59950697991051	36.0	36.0	36.0	32.0	36.0
42	34.54338322033335	36.0	36.0	36.0	32.0	36.0
43	34.53162949968404	36.0	36.0	36.0	32.0	36.0
44	34.47808724905829	36.0	36.0	36.0	32.0	36.0
45	34.456425714037024	36.0	36.0	36.0	32.0	36.0
46	34.445676149418986	36.0	36.0	36.0	32.0	36.0
47	34.41705168749872	36.0	36.0	36.0	32.0	36.0
48	34.377901986162605	36.0	36.0	36.0	32.0	36.0
49	34.36529979258764	36.0	36.0	36.0	32.0	36.0
50	33.77536803132344	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	5.0
19	7.0
20	30.0
21	82.0
22	272.0
23	850.0
24	2113.0
25	4797.0
26	9910.0
27	17740.0
28	29648.0
29	45589.0
30	66192.0
31	94992.0
32	138203.0
33	224242.0
34	477434.0
35	1714146.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.6928253056984	17.791441435703195	11.988196694395022	26.527536564203384
2	16.303286345108695	19.889322916666664	36.448426177536234	27.358964560688403
3	18.08153424611004	23.488490748157982	28.91791520064764	29.51205980508434
4	12.465404737153845	15.8742632473939	35.5320505517197	36.128281463732556
5	14.561677754370272	36.532845243208854	33.27195644835885	15.63352055406202
6	34.97518975661052	35.39447296277897	16.26344713776408	13.366890142846428
7	30.727525616090006	30.371612429327755	20.25885174388262	18.642010210699617
8	28.567354526521676	32.66666761020064	18.829871625126405	19.93610623815128
9	27.447882647410783	13.95904349912144	18.06428237312196	40.528791480345824
10	15.923967810053272	26.666109975713177	31.3630129812782	26.04690923295535
11	37.70032700528685	20.834857730409226	22.081490198687025	19.38332506561689
12	24.73309193016622	23.420647967238615	28.388212807482976	23.458047295112188
13	29.62999918265878	19.170048371739526	25.071287369035222	26.12866507656647
14	23.802723610949926	19.184516008503145	24.856169734573893	32.15659064597304
15	25.50178194335147	26.874331240481673	21.821304625975895	25.80258219019096
16	26.201920014945717	25.679952219000025	23.092616731558813	25.025511034495445
17	24.549881220866915	25.81671711035172	24.555967014995822	25.07743465378554
18	25.264157919784026	24.91928336007671	25.42299057583472	24.393568144304545
19	25.980370476386934	24.65150855213599	24.901769232973837	24.466351738503242
20	26.183985147752896	24.00182716323708	24.435548444416767	25.378639244593256
21	27.254521355830015	23.907865322791228	24.091500622378597	24.74611269900016
22	26.311755860238833	23.881220698805837	24.588872180451126	25.2181512605042
23	24.97841652289476	23.825752697226985	25.111349512213415	26.084481267664838
24	25.21953149159698	24.63607201920852	24.74572277147849	25.398673717716008
25	25.370267234967635	24.154784406776304	24.84341175896132	25.63153659929474
26	25.21065286198801	24.811975535146555	25.005104216241847	24.972267386623592
27	25.815205371988704	24.45469268775298	24.34761808548771	25.382483854770605
28	25.162217839327024	24.33777119164032	25.17078076159055	25.329230207442098
29	25.15030847204786	24.50261624492072	24.993064863342422	25.354010419688993
30	25.010385182343985	24.66241965194955	25.24926206821879	25.07793309748767
31	25.648254172251605	24.34640268473475	24.39229709856719	25.61304604444646
32	25.473370153469126	24.375807408681215	24.230911717656152	25.919910720193506
33	25.07330719385334	24.201263852467747	24.678490411415506	26.046938542263405
34	25.71208992466177	24.15883161346662	24.94126363851237	25.18781482335924
35	26.094465521231175	24.082310572526673	24.834564326187984	24.988659580054165
36	25.11613390855357	24.716706864143852	24.52017800122291	25.646981226079667
37	25.98566035572763	24.330264023401437	24.500707853189727	25.183367767681204
38	25.180243315648994	24.185176365098737	24.98824327035595	25.646337048896317
39	25.84436603371944	24.173962827088783	24.362205181335415	25.619465957856356
40	25.955136805991952	24.37495400170972	24.730202335863947	24.93970685643438
41	25.084593792338914	24.735320416127507	24.982370027383514	25.197715764150065
42	26.15185980386468	24.66156112846453	24.590264283075722	24.59631478459507
43	25.53106419475963	23.851749267217038	24.906907195921608	25.710279342101728
44	25.185230003651526	24.383309697489505	24.603215040888596	25.828245257970373
45	25.314615786075063	24.57083211704659	24.6390857061982	25.475466390680147
46	25.161628399043817	24.40125311545108	24.806031768047678	25.631086717457425
47	25.250494833002264	24.35130347893961	25.002176062183718	25.396025625874408
48	25.888985838925656	24.837573305196592	24.269707925630122	25.003732930247637
49	25.229845363888803	24.817390405937484	24.489889068475236	25.462875161698477
50	25.200170262814027	24.948208189103536	24.519225155587733	25.332396392494704
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	31.0
1	41.0
2	51.0
3	149.5
4	248.0
5	314.5
6	381.0
7	337.5
8	294.0
9	417.0
10	540.0
11	773.0
12	1006.0
13	1955.0
14	2904.0
15	3844.5
16	4785.0
17	4723.5
18	4662.0
19	4747.5
20	4833.0
21	5673.5
22	6514.0
23	7855.5
24	9197.0
25	11645.0
26	14093.0
27	17434.5
28	20776.0
29	24588.5
30	28401.0
31	32423.5
32	36446.0
33	42680.0
34	48914.0
35	57123.5
36	65333.0
37	78425.5
38	91518.0
39	102597.5
40	113677.0
41	126623.5
42	139570.0
43	147081.5
44	154593.0
45	169842.0
46	185091.0
47	199078.5
48	213066.0
49	221174.0
50	229282.0
51	225997.5
52	222713.0
53	220079.0
54	217445.0
55	216313.5
56	215182.0
57	208419.0
58	201656.0
59	184609.0
60	167562.0
61	147014.0
62	126466.0
63	110268.0
64	94070.0
65	79870.5
66	65671.0
67	56387.5
68	47104.0
69	41909.0
70	36714.0
71	28376.5
72	20039.0
73	17078.0
74	14117.0
75	10963.0
76	7809.0
77	6618.0
78	5427.0
79	4555.0
80	3683.0
81	2884.5
82	2086.0
83	1649.0
84	1212.0
85	867.0
86	522.0
87	377.0
88	232.0
89	175.5
90	119.0
91	94.5
92	70.0
93	54.0
94	38.0
95	49.5
96	61.0
97	46.5
98	32.0
99	25.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009411751385402728
2	4.953553360738278E-4
3	2.1229514403164047E-4
4	0.0
5	0.0
6	7.076504801054682E-5
7	1.0614757201582023E-4
8	0.0
9	0.001273770864189843
10	0.0010614757201582023
11	0.0
12	0.0
13	6.01502908089648E-4
14	2.1229514403164047E-4
15	0.00445819802466445
16	9.199456241371087E-4
17	0.0
18	3.1844271604746074E-4
19	3.538252400527341E-5
20	5.307378600791011E-4
21	0.0
22	1.4153009602109365E-4
23	6.368854320949215E-4
24	2.830601920421873E-4
25	0.0018398912482742173
26	0.005625821316838473
27	0.0066165319889861275
28	0.004104372784611716
29	0.0023352465843480452
30	0.003962842688590622
31	0.007147269849065229
32	0.0029367494924376933
33	0.004705875692701364
34	0.0023352465843480452
35	0.002830601920421873
36	0.007288799945086323
37	0.0036443999725431614
38	0.007536477613123237
39	0.0038920776405800754
40	0.0021937164883269514
41	0.0035736349245326145
42	0.0014860660082214833
43	0.0014860660082214833
44	0.0013445359122003897
45	0.001026093196152929
46	6.368854320949215E-4
47	0.0016983611522531237
48	0.001981421344295311
49	2.1229514403164047E-4
50	0.0012383883401845693
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2826254.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.805026964151494
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.22045986381808	42.08653952121061
2	12.568832431407506	13.525327357595653
3	3.7682561996839734	6.082533792954817
4	1.69551793176359	3.649095521469693
5	0.922315559909567	2.481260678519537
6	0.58013929159688	1.8728646136400326
7	0.4019651515880353	1.5139422073890436
8	0.27787291274583914	1.1960767650317754
9	0.22297179258806427	1.079730298120139
>10	1.1994553137134034	11.498815513073115
>50	0.07648285149190259	2.851852697829159
>100	0.053704828829919816	6.016741741718085
>500	0.008457755332544184	3.1482294399702857
>1k	0.003568115530871992	2.996989851478028
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.538252400527341E-5	3.538252400527341E-5
5	0.0	7.076504801054682E-5	0.0	3.538252400527341E-5	3.538252400527341E-5
6	0.0	7.076504801054682E-5	0.0	3.538252400527341E-5	3.538252400527341E-5
7	0.0	7.076504801054682E-5	0.0	3.538252400527341E-5	3.538252400527341E-5
8	0.0	7.076504801054682E-5	0.0	1.0614757201582023E-4	3.538252400527341E-5
9	0.0	7.076504801054682E-5	0.0	1.0614757201582023E-4	3.538252400527341E-5
10	0.0	7.076504801054682E-5	0.0	1.0614757201582023E-4	3.538252400527341E-5
11	0.0	7.076504801054682E-5	0.0	1.0614757201582023E-4	1.0614757201582023E-4
12	0.0	7.076504801054682E-5	0.0	1.4153009602109365E-4	3.184427160474607E-4
13	0.0	1.0614757201582023E-4	0.0	1.7691262002636704E-4	4.2459028806328094E-4
14	0.0	1.0614757201582023E-4	0.0	1.7691262002636704E-4	4.2459028806328094E-4
15	0.0	1.0614757201582023E-4	0.0	1.7691262002636704E-4	4.5997281206855433E-4
16	0.0	1.0614757201582023E-4	0.0	2.1229514403164047E-4	5.307378600791011E-4
17	0.0	1.0614757201582023E-4	0.0	2.1229514403164047E-4	5.307378600791011E-4
18	0.0	1.0614757201582023E-4	0.0	2.830601920421873E-4	6.01502908089648E-4
19	0.0	1.0614757201582023E-4	0.0	2.830601920421873E-4	6.01502908089648E-4
20	0.0	1.0614757201582023E-4	0.0	3.184427160474607E-4	6.722679561001948E-4
21	0.0	1.0614757201582023E-4	0.0	3.184427160474607E-4	7.076504801054682E-4
22	0.0	1.0614757201582023E-4	0.0	4.2459028806328094E-4	7.076504801054682E-4
23	0.0	1.0614757201582023E-4	0.0	6.01502908089648E-4	7.430330041107416E-4
24	0.0	1.0614757201582023E-4	0.0	7.784155281160151E-4	7.430330041107416E-4
25	0.0	1.0614757201582023E-4	0.0	8.491805761265619E-4	7.784155281160151E-4
26	0.0	1.0614757201582023E-4	0.0	0.0010614757201582023	8.491805761265619E-4
27	0.0	1.0614757201582023E-4	0.0	0.0012737708641898428	8.491805761265619E-4
28	0.0	1.0614757201582023E-4	0.0	0.0028659844444271464	8.845631001318353E-4
29	0.0	1.0614757201582023E-4	0.0	0.007076504801054683	8.845631001318353E-4
30	0.0	1.0614757201582023E-4	0.0	0.013339211549988076	8.845631001318353E-4
31	0.0	1.0614757201582023E-4	0.0	0.03251653956084626	9.199456241371087E-4
32	0.0	1.0614757201582023E-4	0.0	0.0505616268035357	9.199456241371087E-4
33	0.0	1.0614757201582023E-4	0.0	0.07055275286651518	9.553281481423821E-4
34	0.0	1.0614757201582023E-4	0.0	0.09539128471821712	0.0011676232921740226
35	0.0	1.0614757201582023E-4	0.0	0.1237680689704464	0.0011676232921740226
36	0.0	1.0614757201582023E-4	0.0	0.16063665898394128	0.0011676232921740226
37	0.0	1.0614757201582023E-4	0.0	0.22096386241293245	0.0011676232921740226
38	0.0	1.0614757201582023E-4	0.0	0.3023790501490666	0.001203005816179296
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3635	0.0	27.11381	1
GGTATCA	1555	0.0	24.051128	1
CGTCGTA	770	0.0	20.856499	10
ACCGTCG	855	0.0	19.55426	8
TACCGTC	880	0.0	18.748425	7
CCGTCGT	895	0.0	18.18906	9
TCAACGC	5380	0.0	18.154608	4
AACGCAG	5445	0.0	17.937887	6
ATCAACG	5505	0.0	17.78234	3
CAACGCA	5520	0.0	17.694166	5
AGTATCG	100	2.486714E-5	17.598522	5
ATACCGT	945	0.0	17.45885	6
TATCAAC	5635	0.0	17.411137	2
GTCGTAG	975	0.0	17.148188	11
CTCGCGT	90	2.2163175E-4	17.111488	24
CGCAATA	750	0.0	16.721554	36
ATACGAA	780	0.0	16.076994	40
TACGAAT	830	0.0	15.638346	41
GTATTAT	340	0.0	15.529206	1
ACGCGAG	100	4.943777E-4	15.401158	37
>>END_MODULE