FastQCFastQC Report
Thu 2 Feb 2017
SRR4064155_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064155_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12186
Sequences flagged as poor quality0
Sequence length25
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT1541.2637452814705399No Hit
TATCAACGCAGAGTACTTTTTTTTT880.7221401608403085No Hit
CTGTAGGACGTGGAATATGGCAAGA520.4267191859510914No Hit
ACGCAGAGTACTTTTTTTTTTTTTT520.4267191859510914No Hit
GTCCTAAAGTGTGTATTTCTCATTT450.3692762186115214No Hit
GTCCTACAGTGGACATTTCTAAATT430.35286394222878714No Hit
GAGTACTTTTTTTTTTTTTTTTTTT410.33645166584605285No Hit
CTTTAGGACGTGAAATATGGCGAGG400.32824552765468573No Hit
GGTATCAACGCAGAGTACTTTTTTT390.32003938946331856No Hit
GTACTTTTTTTTTTTTTTTTTTTTT310.25439028393238144No Hit
ATTTAGAAATGTCCACTGTAGGACG290.23797800754964712No Hit
GTCCTACAGTGTGCATTTCTCATTT260.2133595929755457No Hit
ATCAACGCAGAGTACTTTTTTTTTT220.18053504021007713No Hit
TTCCAGGTCCTTCAGTGTGCATTTC190.1559166256359757No Hit
CTGTAGGACCTGGAATATGGCGAGA150.12309207287050715No Hit
TTTCTAAATTTTCCACCTTTTTCAG150.12309207287050715No Hit
GCAGAGTACTTTTTTTTTTTTTTTT150.12309207287050715No Hit
GTCCTTCAGTGTGCATTTCTCATTT140.11488593467914No Hit
CTGTAGGACATGGAATATGGCAAGA140.11488593467914No Hit
GAATATGGCAAGAAAACTGAAAATC140.11488593467914No Hit
CTACAGTGGACATTTCTAAATTTTC130.10667979648777284No Hit
GCCATATTCCACGTCCTACAGTGGA130.10667979648777284No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG130.10667979648777284No Hit
ATTCCAGGTCCTTCAGTGTGCATTT130.10667979648777284No Hit
TCCTAAAGTGTGTATTTCTCATTTT130.10667979648777284No Hit
TTGTAGAACAGTGTATATCAATGAG130.10667979648777284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA501.7673301E-719.01
TATCAAC705.9544054E-613.5714282
AACGCAG654.638443E-513.1538466
TCAACGC654.638443E-513.1538464
ATCAACG654.638443E-513.1538463
CAACGCA654.638443E-513.1538465
CAGAGTA802.3595281E-511.87510
AGAGTAC802.3595281E-511.87511
ACGCAGA751.7705817E-411.47
GCAGAGT751.7705817E-411.49
CGCAGAG751.7705817E-411.48
Produced by FastQC (version 0.11.5)