##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064155_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12186
Sequences flagged as poor quality	0
Sequence length	25
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.413589364844903	32.0	32.0	32.0	14.0	32.0
2	28.21524700475956	32.0	32.0	32.0	14.0	32.0
3	28.298211061874284	32.0	32.0	32.0	14.0	32.0
4	28.469965534219597	32.0	32.0	32.0	14.0	32.0
5	28.363860167405218	32.0	21.0	32.0	14.0	32.0
6	31.597488921713442	36.0	32.0	36.0	21.0	36.0
7	31.58427703922534	36.0	32.0	36.0	21.0	36.0
8	31.2977186935828	36.0	32.0	36.0	14.0	36.0
9	31.951583784670934	36.0	32.0	36.0	14.0	36.0
10	30.80690956835713	36.0	27.0	36.0	14.0	36.0
11	32.08673888068275	36.0	32.0	36.0	21.0	36.0
12	31.410224848186445	36.0	32.0	36.0	14.0	36.0
13	31.645905137042508	36.0	32.0	36.0	14.0	36.0
14	31.27827014606926	36.0	32.0	36.0	14.0	36.0
15	30.810356146397506	36.0	27.0	36.0	14.0	36.0
16	31.042425734449367	36.0	32.0	36.0	14.0	36.0
17	30.93960282291154	36.0	32.0	36.0	14.0	36.0
18	30.978664040702444	36.0	32.0	36.0	14.0	36.0
19	31.158870835384867	36.0	32.0	36.0	14.0	36.0
20	30.927785983915967	36.0	32.0	36.0	14.0	36.0
21	30.87551288363696	36.0	32.0	36.0	14.0	36.0
22	30.757426555063187	36.0	32.0	36.0	14.0	36.0
23	30.54603643525357	36.0	27.0	36.0	14.0	36.0
24	30.735023797800753	36.0	32.0	36.0	14.0	36.0
25	29.70745117347776	36.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	1.0
11	0.0
12	1.0
13	0.0
14	12.0
15	33.0
16	44.0
17	78.0
18	100.0
19	153.0
20	188.0
21	245.0
22	228.0
23	301.0
24	343.0
25	406.0
26	479.0
27	495.0
28	566.0
29	645.0
30	710.0
31	898.0
32	1059.0
33	1258.0
34	1878.0
35	2062.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.753899195534395	23.075028730914465	13.92218026596618	24.24889180758496
2	11.694706606483381	22.486663931062782	46.71317193270414	19.105457529749692
3	16.765140324963074	29.2959133431807	31.790579353356314	22.14836697849992
4	9.929427211554243	17.831938289840803	42.78680452978828	29.45182996881667
5	9.519120301985886	42.6308879041523	36.59937633349746	11.250615460364353
6	26.508001641362334	41.329503487894954	20.041034058268362	12.121460812474353
7	23.963890028723842	35.05129257283545	25.194911776774724	15.789905621665984
8	22.779510753570843	40.25611558036447	21.868330323428008	15.096043342636678
9	26.66064537318335	14.557845471713604	22.957549880942608	35.82395927416044
10	13.639349646904254	30.325176547873212	37.14074560683199	18.89472819839054
11	30.741440183923146	23.78684621069053	29.008949831677477	16.462763773708843
12	23.090819510592873	26.34258498932501	35.23567088191821	15.330924618163902
13	27.467564460502548	25.381836097881425	28.25587124322549	18.89472819839054
14	19.377617209951556	24.345184333689136	31.16840463092208	25.108793825437225
15	18.926020198702684	36.406929961408984	26.808440758682977	17.858609081205355
16	17.868287075053374	30.957464279848907	32.83790441780259	18.336344227295122
17	17.29206010345677	31.11092864767222	32.588882502668525	19.00812874620248
18	17.544147843942508	28.01642710472279	37.478439425051334	16.960985626283367
19	20.55852156057495	26.718685831622174	33.45379876796714	19.26899383983573
20	21.598094768826474	28.176069639484275	33.292272316662554	16.93356327502669
21	19.714262254700714	27.53920683143115	31.60357993267099	21.142950981197142
22	20.960985626283367	30.99794661190965	31.876796714579054	16.164271047227928
23	17.757700205338807	31.342915811088297	33.72484599589322	17.17453798767967
24	19.857107661985708	28.70986285620432	33.941036380060766	17.4919931017492
25	18.610381077529567	29.139290407358736	34.23127463863337	18.01905387647832
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	3.0
2	8.5
3	14.0
4	14.0
5	14.0
6	30.0
7	46.0
8	46.0
9	46.0
10	61.5
11	77.0
12	77.0
13	77.0
14	89.5
15	102.0
16	102.0
17	102.0
18	180.5
19	259.0
20	259.0
21	259.0
22	429.0
23	599.0
24	599.0
25	599.0
26	865.0
27	1131.0
28	1131.0
29	1131.0
30	1410.0
31	1689.0
32	1689.0
33	1689.0
34	1692.5
35	1696.0
36	1696.0
37	1696.0
38	1687.5
39	1679.0
40	1679.0
41	1679.0
42	1592.0
43	1505.0
44	1505.0
45	1505.0
46	1430.0
47	1355.0
48	1355.0
49	1355.0
50	1170.0
51	985.0
52	985.0
53	985.0
54	751.0
55	517.0
56	517.0
57	517.0
58	389.5
59	262.0
60	262.0
61	262.0
62	195.0
63	128.0
64	128.0
65	128.0
66	95.5
67	63.0
68	63.0
69	63.0
70	51.0
71	39.0
72	39.0
73	39.0
74	26.0
75	13.0
76	13.0
77	13.0
78	8.5
79	4.0
80	4.0
81	4.0
82	3.0
83	2.0
84	2.0
85	2.0
86	1.5
87	1.0
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.5
95	2.0
96	2.0
97	2.0
98	8.0
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03282455276546857
2	0.008206138191367143
3	0.0
4	0.0
5	0.0
6	0.008206138191367143
7	0.008206138191367143
8	0.03282455276546857
9	0.05744296733957
10	0.06564910553093714
11	0.05744296733957
12	0.06564910553093714
13	0.06564910553093714
14	0.05744296733957
15	0.05744296733957
16	0.06564910553093714
17	0.05744296733957
18	0.09026752010503857
19	0.09026752010503857
20	0.07385524372230429
21	0.05744296733957
22	0.09026752010503857
23	0.09026752010503857
24	0.07385524372230429
25	0.08206138191367143
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	12186.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.566551780732
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.70575409991469	82.84917118004267
2	2.625841311972699	4.546200558017397
3	0.6446108635889658	1.674052191038897
4	0.3223054317944829	1.1160347940259314
5	0.09479571523367143	0.41030690956835714
6	0.12323442980377286	0.6400787789266371
7	0.06635700066357	0.40210077137699
8	0.06635700066357	0.45954373871656
9	0.028438714570101435	0.22156573116691286
>10	0.2843871457010143	4.841621532906614
>50	0.028438714570101435	1.5755785327424916
>100	0.009479571523367144	1.2637452814705399
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	154	1.2637452814705399	No Hit
TATCAACGCAGAGTACTTTTTTTTT	88	0.7221401608403085	No Hit
CTGTAGGACGTGGAATATGGCAAGA	52	0.4267191859510914	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	52	0.4267191859510914	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	45	0.3692762186115214	No Hit
GTCCTACAGTGGACATTTCTAAATT	43	0.35286394222878714	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	41	0.33645166584605285	No Hit
CTTTAGGACGTGAAATATGGCGAGG	40	0.32824552765468573	No Hit
GGTATCAACGCAGAGTACTTTTTTT	39	0.32003938946331856	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	31	0.25439028393238144	No Hit
ATTTAGAAATGTCCACTGTAGGACG	29	0.23797800754964712	No Hit
GTCCTACAGTGTGCATTTCTCATTT	26	0.2133595929755457	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	22	0.18053504021007713	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	19	0.1559166256359757	No Hit
CTGTAGGACCTGGAATATGGCGAGA	15	0.12309207287050715	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	15	0.12309207287050715	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	15	0.12309207287050715	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	14	0.11488593467914	No Hit
CTGTAGGACATGGAATATGGCAAGA	14	0.11488593467914	No Hit
GAATATGGCAAGAAAACTGAAAATC	14	0.11488593467914	No Hit
CTACAGTGGACATTTCTAAATTTTC	13	0.10667979648777284	No Hit
GCCATATTCCACGTCCTACAGTGGA	13	0.10667979648777284	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG	13	0.10667979648777284	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	13	0.10667979648777284	No Hit
TCCTAAAGTGTGTATTTCTCATTTT	13	0.10667979648777284	No Hit
TTGTAGAACAGTGTATATCAATGAG	13	0.10667979648777284	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	50	1.7673301E-7	19.0	1
TATCAAC	70	5.9544054E-6	13.571428	2
AACGCAG	65	4.638443E-5	13.153846	6
TCAACGC	65	4.638443E-5	13.153846	4
ATCAACG	65	4.638443E-5	13.153846	3
CAACGCA	65	4.638443E-5	13.153846	5
CAGAGTA	80	2.3595281E-5	11.875	10
AGAGTAC	80	2.3595281E-5	11.875	11
ACGCAGA	75	1.7705817E-4	11.4	7
GCAGAGT	75	1.7705817E-4	11.4	9
CGCAGAG	75	1.7705817E-4	11.4	8
>>END_MODULE