FastQCFastQC Report
Thu 2 Feb 2017
SRR4064155_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064155_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12186
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA210.17232890201870998No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC180.14771048744460857No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA150.12309207287050715No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACACTT140.11488593467914No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT140.11488593467914No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTAGA250.002262597435.21
TTAGAAA300.005526851429.3333323
GAAATGT300.005526851429.3333326
TTTAGAA300.005526851429.3333322
TATCAAC500.002437678222.02
ATCAACG500.002437678222.03
AACGCAG550.004237492620.0000026
GTATCAA550.004237492620.0000021
CTCATTT550.004237492620.00000219
TTTCTCA600.00700841418.33333216
TTCTCAT600.00700841418.33333217
TCTCATT600.00700841418.33333218
ATTTCTC600.00700841418.33333215
Produced by FastQC (version 0.11.5)