##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064155_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12186
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.572952568521252	32.0	32.0	32.0	21.0	32.0
2	29.65263417035943	32.0	32.0	32.0	21.0	32.0
3	30.86591170195306	32.0	32.0	32.0	32.0	32.0
4	31.16715903495815	32.0	32.0	32.0	32.0	32.0
5	30.770556376169374	32.0	32.0	32.0	32.0	32.0
6	34.37198424421467	36.0	36.0	36.0	32.0	36.0
7	34.44510093549975	36.0	36.0	36.0	32.0	36.0
8	34.29361562448712	36.0	36.0	36.0	32.0	36.0
9	34.622353520433286	36.0	36.0	36.0	32.0	36.0
10	34.26776628918431	36.0	36.0	36.0	32.0	36.0
11	34.77268997209913	36.0	36.0	36.0	32.0	36.0
12	34.47004759560151	36.0	36.0	36.0	32.0	36.0
13	34.62727720334811	36.0	36.0	36.0	32.0	36.0
14	34.5510421795503	36.0	36.0	36.0	32.0	36.0
15	34.47735105859183	36.0	36.0	36.0	32.0	36.0
16	34.485803380928935	36.0	36.0	36.0	32.0	36.0
17	34.49310684391925	36.0	36.0	36.0	32.0	36.0
18	34.51337600525193	36.0	36.0	36.0	32.0	36.0
19	34.45281470539964	36.0	36.0	36.0	32.0	36.0
20	34.51116034794026	36.0	36.0	36.0	32.0	36.0
21	34.48284917118004	36.0	36.0	36.0	32.0	36.0
22	34.45757426555063	36.0	36.0	36.0	32.0	36.0
23	34.408419497784344	36.0	36.0	36.0	32.0	36.0
24	34.42573444936813	36.0	36.0	36.0	32.0	36.0
25	34.388150336451666	36.0	36.0	36.0	32.0	36.0
26	34.3457246020023	36.0	36.0	36.0	32.0	36.0
27	34.262924667651404	36.0	36.0	36.0	32.0	36.0
28	34.21803709174463	36.0	36.0	36.0	32.0	36.0
29	34.266781552601344	36.0	36.0	36.0	32.0	36.0
30	34.11316264565895	36.0	36.0	36.0	32.0	36.0
31	34.14089939274577	36.0	36.0	36.0	32.0	36.0
32	34.08362054817003	36.0	36.0	36.0	32.0	36.0
33	34.1506646971935	36.0	36.0	36.0	32.0	36.0
34	34.155916625635975	36.0	36.0	36.0	32.0	36.0
35	34.08271787296898	36.0	36.0	36.0	32.0	36.0
36	34.0552273100279	36.0	36.0	36.0	32.0	36.0
37	33.92056458230756	36.0	36.0	36.0	32.0	36.0
38	33.80928934843263	36.0	36.0	36.0	32.0	36.0
39	33.71409814541277	36.0	36.0	36.0	27.0	36.0
40	33.81987526669949	36.0	36.0	36.0	32.0	36.0
41	33.80395535860824	36.0	36.0	36.0	32.0	36.0
42	33.74577383883145	36.0	36.0	36.0	27.0	36.0
43	33.70695880518628	36.0	36.0	36.0	27.0	36.0
44	33.51649433776465	36.0	36.0	36.0	21.0	36.0
45	33.57754800590842	36.0	36.0	36.0	21.0	36.0
46	33.46446742163138	36.0	36.0	36.0	21.0	36.0
47	33.34006236665025	36.0	36.0	36.0	21.0	36.0
48	33.33891350730346	36.0	36.0	36.0	21.0	36.0
49	33.29016904644674	36.0	36.0	36.0	21.0	36.0
50	32.53906121779091	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	0.0
23	5.0
24	11.0
25	23.0
26	56.0
27	115.0
28	224.0
29	336.0
30	501.0
31	833.0
32	1154.0
33	1797.0
34	2852.0
35	4278.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.08353848678812	24.396848842934517	14.771048744460858	23.74856392581651
2	13.064172000656491	25.50467749876908	42.74577383883145	18.685376661742982
3	17.618578696865256	30.830461184966357	32.635811587067124	18.915148531101263
4	12.259970457902511	21.278516330214998	41.39176103725587	25.06975217462662
5	12.973904480551452	40.37419990152634	35.5900213359593	11.061874281962908
6	26.875102576727393	43.42688330871492	18.644345970786148	11.053668143771542
7	23.66650254390284	34.85967503692763	24.790743476120138	16.683078943049402
8	22.86230100114886	41.34252420810767	21.762678483505663	14.032496307237812
9	25.791892335466933	15.501395043492533	22.107336287543085	36.59937633349746
10	13.195470211718366	32.00393894633186	35.87723617265715	18.92335466929263
11	31.70031183325127	23.026423764976204	28.491711800426717	16.781552601345805
12	22.599704579025108	26.694567536517315	34.37551288363696	16.330215000820616
13	27.884457574265554	25.004103069095684	26.989988511406533	20.121450845232232
14	18.020679468242246	24.946660101756112	30.4940095191203	26.53865091088134
15	19.48297086581863	36.8321707016824	25.21953221173574	18.465326220763234
16	18.250451337600527	31.093057607090103	31.749548662399473	18.906942392909897
17	18.29148202855736	32.04496963728869	30.05087805678648	19.61267027736747
18	18.32430658132283	29.80469391104546	34.10471032332185	17.766289184309862
19	21.139011980961758	28.097817167241097	31.10946988347284	19.653700968324305
20	21.8529460036107	30.48580338092893	31.6921056950599	15.96914492040046
21	21.02412604628262	28.943049400951914	29.279501066797962	20.753323485967503
22	21.040538322665352	31.757754800590842	29.960610536681436	17.241096340062366
23	18.307894304940096	32.356802888560644	30.25603151157065	19.079271294928606
24	20.86992203528929	30.094378334017236	31.66187935986869	17.373820270824787
25	19.194157229607747	31.560807483998033	31.486952240275727	17.758083046118497
26	17.823732151649434	31.199737403577878	32.06958805186279	18.906942392909897
27	18.72640735269982	31.979320531757754	30.78943049400952	18.504841621532904
28	18.266863613983258	31.93008370260955	30.173970129656986	19.629082553750205
29	19.33366157886099	30.928934843262763	30.026259642212377	19.711143935663877
30	18.250451337600527	30.87969801411456	30.66633842113901	20.203512227145907
31	19.310627821091504	30.422650800164135	30.750923266311037	19.51579811243332
32	18.389955686853767	32.27474150664697	30.920728705071394	18.41457410142787
33	17.77449532250123	31.946495978992285	32.586574757918925	17.69243394058756
34	19.834236008534383	31.897259149844082	31.084851468898737	17.183653372722794
35	18.46381093057607	32.75890365993763	30.280649926144754	18.49663548334154
36	17.79911373707533	31.26538650910881	31.544395207615295	19.39110454620056
37	18.143771541112752	31.93008370260955	29.58312817987855	20.343016576399148
38	18.892080426754205	32.802626179729174	29.396799343455065	18.90849405006155
39	20.457902511078284	30.72378138847858	29.624158870835387	19.194157229607747
40	21.278516330214998	30.141145576891514	31.314623338257014	17.26571475463647
41	20.211718365337273	29.894961431150502	32.02855736090596	17.864762842606268
42	18.685376661742982	32.578368619727556	31.232561956343346	17.503692762186116
43	17.92220580994584	33.14459215493189	30.379123584441164	18.55407845068111
44	17.076973576235023	32.28294764483834	30.764812079435416	19.87526669949122
45	17.717052355161663	32.68504841621533	29.903167569341864	19.694731659281143
46	19.15312653865091	30.48580338092893	30.141145576891514	20.219924503528638
47	20.950270802560315	30.838667323157722	29.361562448711638	18.849499425570325
48	18.309396799343457	31.267952400492412	30.33237587197374	20.0902749281904
49	19.711143935663877	30.764812079435416	30.518627933694404	19.005416051206304
50	18.906942392909897	32.1434432955851	29.68980797636632	19.259806335138684
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.0
4	1.0
5	2.5
6	4.0
7	3.5
8	3.0
9	7.0
10	11.0
11	15.0
12	19.0
13	27.5
14	36.0
15	48.0
16	60.0
17	61.5
18	63.0
19	77.5
20	92.0
21	111.0
22	130.0
23	164.0
24	198.0
25	252.5
26	307.0
27	351.0
28	395.0
29	501.5
30	608.0
31	748.0
32	888.0
33	1016.5
34	1145.0
35	1250.5
36	1356.0
37	1322.5
38	1289.0
39	1204.0
40	1119.0
41	1013.5
42	908.0
43	849.5
44	791.0
45	744.0
46	697.0
47	611.5
48	526.0
49	464.5
50	403.0
51	372.5
52	342.0
53	289.0
54	236.0
55	212.5
56	189.0
57	147.0
58	105.0
59	92.0
60	79.0
61	69.0
62	59.0
63	50.5
64	42.0
65	31.0
66	20.0
67	19.0
68	18.0
69	16.0
70	14.0
71	13.0
72	12.0
73	9.5
74	7.0
75	6.0
76	5.0
77	2.5
78	0.0
79	0.5
80	1.0
81	1.5
82	2.0
83	1.5
84	1.0
85	1.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	1.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.008206138191367143
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.008206138191367143
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.008206138191367143
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.008206138191367143
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.008206138191367143
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	12186.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.69177744953224
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.74461881485293	93.53356310520269
2	1.5779092702169626	3.0198588544231084
3	0.2829945973758683	0.8124076809453471
4	0.13720950175799676	0.5251928442474971
5	0.06860475087899838	0.32824552765468573
6	0.05145356315924878	0.29542097488921715
7	0.02572678157962439	0.17232890201870998
8	0.02572678157962439	0.19694731659281145
9	0.0	0.0
>10	0.08575593859874796	1.1160347940259314
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	21	0.17232890201870998	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	18	0.14771048744460857	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	15	0.12309207287050715	No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACACTT	14	0.11488593467914	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT	14	0.11488593467914	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.008206138191367143	0.0
36	0.0	0.0	0.0	0.008206138191367143	0.0
37	0.0	0.0	0.0	0.049236829148202856	0.0
38	0.0	0.0	0.0	0.10667979648777286	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTAGA	25	0.0022625974	35.2	1
TTAGAAA	30	0.0055268514	29.333332	3
GAAATGT	30	0.0055268514	29.333332	6
TTTAGAA	30	0.0055268514	29.333332	2
TATCAAC	50	0.0024376782	22.0	2
ATCAACG	50	0.0024376782	22.0	3
AACGCAG	55	0.0042374926	20.000002	6
GTATCAA	55	0.0042374926	20.000002	1
CTCATTT	55	0.0042374926	20.000002	19
TTTCTCA	60	0.007008414	18.333332	16
TTCTCAT	60	0.007008414	18.333332	17
TCTCATT	60	0.007008414	18.333332	18
ATTTCTC	60	0.007008414	18.333332	15
>>END_MODULE